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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 333 results for: uros

Showing: 101 - 120 of 333 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...in green monkeys and cultured human neurons, with broader neuronal expression than AAV9. This new capsid...https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate...-brain barrier and delivering a transgene, with neuron bias observed in young macaques and vascular bias...

  2. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ... expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated...Vectors    Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest....1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...channelrhodopsin for activation of CNS neurons. Current protocols in neuroscience / editorial board, Jacqueline... Simon. The surgeries and standard molecular neuroscience validation experiments we discussed last week...optogenetic molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures...

  4. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...expressing validated antibodies for neuroscience research (see the NeuroMab Collection for details). The following..., I wanted to find a specific plasmid from the NeuroMab collection for an antibody against PARIS, a zinc...zinc finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... rare genetic eye disease, Leber’s congenital amaurosis. This is the first of what I, scientists, doctors...childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 ...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway...

  6. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...Domains Tweeted by Biologists. 7. Biologists, neuroscientists and ecologists (re)tweet scientific domains...Domains, Categorized by Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing...however, ~20% of life scientists  (biologists, neuroscientists, ecologists) tweet scientific domains 20% or...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...iPSCs) to take on a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven...EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned ...a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral... voltage indicator for use in the detection of neuronal action potentials. The Cohen lab wished to improve...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

  8. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following the development of adenine...high product purity and low off-target editing Thuronyi et al., 2019 evoAPOBEC1-BE4max   Improved editing... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H....

  9. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f in the...optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an optogenetic tool...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/...

  10. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...heard of a Freshers Fair? If you’re from outside of Europe, the answer is probably no, but these fun and informative... of the main ways budding young scholars across Europe get introduced to the cultures and opportunities... and scientific community activities throughout Europe and the world. So get in touch! Resources at Addgene...

  11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana...expression, while the human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer...

  12. What the HEK?

    Type
    Blog Post
    ... Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent with kidney...is next to the kidney and is known to have some neuronal qualities.) While HEKs probably aren’t a great..., et al., Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...

  13. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...already available allowing you to chemically control neuronal activity while studying your phenotype of interest...interest. Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We...we have retrograde AAV that you can use to map neuronal connections. Expanding the viral vector knowledge...

  14. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...including neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and ...and cell death post-transduction is low. Neuronal stem cells transduced with iTOP resulting in GFP...

  18. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar... are urgently needed. Therefore, understanding neuronal networks, their interactions and information processing...microscope will allow Tsakanova to image cortical neurons and study changes in neural activity after ischemic...

  19. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...region and ~75% of neurons were co-transduced with Cpf1 and GFP.  Of these neurons, ~15% had indels at...multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo. For these experiments, cells were ...

  20. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine...affected signaling pathways and how they lead to neurological phenotypes observed.” Using genetically-encoded...genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes to...
Showing: 101 - 120 of 333 results