... expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated...Vectors    Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest....1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

...channelrhodopsin for activation of CNS neurons. Current protocols in neuroscience / editorial board, Jacqueline... Simon. The surgeries and standard molecular neuroscience validation experiments we discussed last week...optogenetic molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures...

... rare genetic eye disease, Leber’s congenital amaurosis. This is the first of what I, scientists, doctors...childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 ...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway...

...expressing validated antibodies for neuroscience research (see the NeuroMab Collection for details). The following..., I wanted to find a specific plasmid from the NeuroMab collection for an antibody against PARIS, a zinc...zinc finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator...

...Domains Tweeted by Biologists. 7. Biologists, neuroscientists and ecologists (re)tweet scientific domains...Domains, Categorized by Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing...however, ~20% of life scientists  (biologists, neuroscientists, ecologists) tweet scientific domains 20% or...

...image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f in the...optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an optogenetic tool...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/...

...heard of a Freshers Fair? If you’re from outside of Europe, the answer is probably no, but these fun and informative... of the main ways budding young scholars across Europe get introduced to the cultures and opportunities... and scientific community activities throughout Europe and the world. So get in touch! Resources at Addgene...

...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

...effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following the development of adenine...high product purity and low off-target editing Thuronyi et al., 2019 evoAPOBEC1-BE4max   Improved editing... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H....

...iPSCs) to take on a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven...EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned ...a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral... voltage indicator for use in the detection of neuronal action potentials. The Cohen lab wished to improve...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

... Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent with kidney...is next to the kidney and is known to have some neuronal qualities.) While HEKs probably aren’t a great..., et al., Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...

...Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana...expression, while the human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer...

...already available allowing you to chemically control neuronal activity while studying your phenotype of interest...interest. Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We...we have retrograde AAV that you can use to map neuronal connections. Expanding the viral vector knowledge...

...including neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and ...and cell death post-transduction is low. Neuronal stem cells transduced with iTOP resulting in GFP...

...started yet! @neurosymp will be interesting this year. Everyone interested in #Neuroscience should come ...networking within the app. For example, the NeuroSymposium had virtual tables that you can join to connect...

...channels that can be used to silence the activity of neurons. Earlier this year, the Hegemann lab and collaborators... cysteine.  With the introduction of MerMAIDs, neurons can be turned off quickly and with high precision... a valuable optogenetics feature when studying neuronal activity. The researchers identified MerMAIDS ...

...genes and wanted to express them within a specific neuronal compartment, you could express them individually...J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections, Cell...binary transcriptional systems in Drosophila., Nat. Neurosci. 9, 703–709 (2006). PubMed PMID: 16582903. 8. ...

...proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar... are urgently needed. Therefore, understanding neuronal networks, their interactions and information processing...microscope will allow Tsakanova to image cortical neurons and study changes in neural activity after ischemic...

...region and ~75% of neurons were co-transduced with Cpf1 and GFP.  Of these neurons, ~15% had indels at...multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo. For these experiments, cells were ...

...think resources like this are why my brand of neuroscience has exploded. Because we have a resource like... visiting Addgene’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed ... recombinant antibodies service, part of the Neuroscience AntiBody Open Resource (NABOR) platform, supported...