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We narrowed to 322 results for: virus

Showing: 101 - 120 of 322 results

  1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post
    ... the most studied viruses. Adenoviruses are non-enveloped double-strand DNA viruses with a genome of around... DT (2012) Adenovirus Strategies for Tissue-Specific Targeting. In: Applications of viruses for cancer...DG, Sontheimer EJ, Weng Z, Gao G, Xue W (2015) Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR...

  2. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus may acquire mutations to escape ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...structures. We have expanded our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral...

  3. Microbiology Resources

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    Collection
    ...Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus (WNV) ... your microbe of interest, including bacteria, viruses, protozoa, fungi, and more. Plasmid...range of microbial fields, including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids...complementation constructs - Lahaye Lab Vaccinia virus ORF library : Expression library that contains each... the predicted open reading frames for vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden...

  4. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ...silencing genes Virus induced gene silencing (VIGS) is a transient technique that uses a virus and the plant's...: PMC6585625. Unver, Turgay, and Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene...

  5. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post
    ...promoter to use, or if and how much the spread of the virus to adjacent areas impacted the experiments we wanted...and compatibility of recombinant adeno-associated virus under conditions commonly encountered in human gene...2019). Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations. Human Gene Therapy...

  6. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ...tricks discovered in positive single-stranded RNA viruses to allow for the efficient translation of multiple...IRES sequences have been discovered - many from viruses, but also some from cellular mRNAs. What they all...5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in...single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes translation of the viral...vectors. These peptides, first discovered in picornaviruses, are short (about 20 amino acids) and produce...eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Pelletier et al (Nature. 1988 Jul 28;334...efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. Kim...

  7. Viral Production

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    ... specific virus in order to generate high quality preparations. After production, all virus preps are ...Resources Lentivirus Production Titer Quality Control Resources Overview of Viral Production Viruses are generated...experiment using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral...preps available from Addgene. After receiving your virus from Addgene, read our detailed recipient instructions...contamination. In the event of contamination, all of the virus produced in the line will be taken off-line and ...preps available from Addgene After receiving your virus from Addgene, read our detailed recipient instructions...instructions for next steps. Plan an experiment using our virus protocols . Browse our plasmid collection of popular...

  8. Beginner's Guide to Viral Vectors

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    Blog Post
    ...quick reference when you're designing your next virus experiment. Subscribe to Viral Vector Blog Posts...important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide...? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the...

  9. What the HEK?

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    Blog Post
    ...will highlight some of those applications here.   Virus production HEKs are the go-to ‘packaging cells’ ...with the SV40 origin of replication. To generate a virus, you need only transfect the ‘helper’ plasmids (...Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment...transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. (2002...

  10. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post
    ...recognition for sharing your data. Materials: What virus and serotype did you use and what animal subject... region and cell class ended up expressing your virus? If you were using a genetic tool, did it operate...

  11. AAV ddPCR Titration

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    Protocol
    ... C = Copies/µL D = Dilution factor of virus V = Volume of virus in reaction mix (4 µL) Sample Data When...Titration by qPCR AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction...Water Procedure Preparation Before handling any viruses, get materials ready. Ensure that primers, probe...contamination of the PCR reagents and the NTC with virus. Pre-warm the 96-well plate sealer by gently touching...plate and pipette 5–10 times to mix. Dilute the virus as follows: Mix dilutions thoroughly, but pipette...

  12. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...service as one of those stories. Viral vectors are viruses engineered to be safely used to deliver a genetic...especially those familiar with adeno-associated viruses (AAVs), will know just how tricky these tools can... Kamens, J., & Fan, M. (2019). Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...

  13. Hot Plasmids Spring 2024

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    Blog Post
    ...the SARS-CoV-2 nucelocapsid protein, one of the virus’s most abundant proteins and a critical component...microglia was further enhanced by packaging the virus as self-complementary AAV (scAAV). However, scAAV...Targeting of Resident Microglia with Adeno-Associated Virus. bioRxiv 2023.12.12.571321; doi: https://doi.org...

  14. Hot Plasmids: Fall 2024

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    Blog Post
    ...epitope derived from the P and V proteins of Simian virus 5 (SV5) (Randall et al., 1987). The epitope (GKPIPNPLLGLDST...matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked...characterization of monoclonal antibodies to simian virus 5 and their use in revealing antigenic differences...

  15. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ...reserve lots, but if you’re reordering the same virus feel free to email us and we can do our best to ...Characterization of a recombinant adeno-associated virus type 2 Reference Standard Material." Human gene ...Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols ...

  16. Plasmids 101: TOPO Cloning

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    Blog Post
    .... The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I as this enzyme recognizes.... Shuman S. "Recombination mediated by vaccinia virus DNA topoisomerase I in Escherichia coli is sequence...

  17. Brain Initiative Collection

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    Collection
    ...100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with ... 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with ...millisecond resolution optogenetics. Cre-dependent virus Edward Boyden 108422-AAV5 pAAV-CAG-FLEX-Archon1-...pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter...pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter ...pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter...

  18. Serotype Testing AAV

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    ...that was used to make the virus. For more details, see our FAQ on What is virus associated DNA, and why ...Cart *Each viral service request will also include virus associated DNA (vdna), which is a sample of the ...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

  19. Lentivirus Plasmids

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    ...Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety Blog: Tips for Titering...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ...considerations to evaluate when working with lentivirus: these viruses are based on HIV-1, which may require ...cells without a significant immune response. These viruses also integrate stably into the host genome, enabling...biosafety procedures. Additionally, because this virus integrates randomly into the host genome, it has...Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and...Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral terms...

  20. Retrovirus Plasmids

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    Collection
    ...Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and ...cell, the virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets...discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses. γ-Retroviruses...Frequently asked questions (FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common...γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV...transgene expression. Some disadvantages of γ-retroviruses are that they can only transduce dividing cells...breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate randomly into the host's genome, which...
Showing: 101 - 120 of 322 results