...of the upstream efficiency element of the simian virus 40 late polyadenylation signal by using in vitro...Donello JE, Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances ...H-G. Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...

...how a phage recognizes its host Bacteriophages (viruses that prey on bacteria) may be the most numerous...Simpson, Sacher, and Szymanski in January 2016 in Viruses (5). Please refer to the paper for more details...Function of Modified Bases in Bacteria and Their Viruses." Chemical Reviews (2016). PubMed PMID: 27319741...Receptor Binding Proteins from Bacteriophages." Viruses 8.1 (2016): 17. PubMed PMID: 26761028. PubMed Central...

...severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus may acquire mutations to escape ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...structures. We have expanded our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral...

... the most studied viruses. Adenoviruses are non-enveloped double-strand DNA viruses with a genome of around... DT (2012) Adenovirus Strategies for Tissue-Specific Targeting. In: Applications of viruses for cancer...DG, Sontheimer EJ, Weng Z, Gao G, Xue W (2015) Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR...

...that was used to make the virus. For more details, see our FAQ on What is virus associated DNA, and why ...Cart *Each viral service request will also include virus associated DNA (vdna), which is a sample of the ...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

...100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with ... 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with ...millisecond resolution optogenetics. Cre-dependent virus Edward Boyden 108422-AAV5 pAAV-CAG-FLEX-Archon1-...pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter...pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter ...pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter...

...silencing genes Virus induced gene silencing (VIGS) is a transient technique that uses a virus and the plant's...: PMC6585625. Unver, Turgay, and Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene...

...promoter to use, or if and how much the spread of the virus to adjacent areas impacted the experiments we wanted...and compatibility of recombinant adeno-associated virus under conditions commonly encountered in human gene...2019). Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations. Human Gene Therapy...

...tricks discovered in positive single-stranded RNA viruses to allow for the efficient translation of multiple...IRES sequences have been discovered - many from viruses, but also some from cellular mRNAs. What they all...5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in...single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes translation of the viral...vectors. These peptides, first discovered in picornaviruses, are short (about 20 amino acids) and produce...eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Pelletier et al (Nature. 1988 Jul 28;334...efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. Kim...

...Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety Blog: Tips for Titering...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ...considerations to evaluate when working with lentivirus: these viruses are based on HIV-1, which may require ...cells without a significant immune response. These viruses also integrate stably into the host genome, enabling...biosafety procedures. Additionally, because this virus integrates randomly into the host genome, it has...Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and...Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral terms...

...Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and ...cell, the virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets...discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses. γ-Retroviruses...Frequently asked questions (FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common...γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV...transgene expression. Some disadvantages of γ-retroviruses are that they can only transduce dividing cells...breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate randomly into the host's genome, which...

...quick reference when you're designing your next virus experiment. Subscribe to Viral Vector Blog Posts...important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide...? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the...

...recognition for sharing your data. Materials: What virus and serotype did you use and what animal subject... region and cell class ended up expressing your virus? If you were using a genetic tool, did it operate...

...will highlight some of those applications here.   Virus production HEKs are the go-to ‘packaging cells’ ...with the SV40 origin of replication. To generate a virus, you need only transfect the ‘helper’ plasmids (...Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment...transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. (2002...

... C = Copies/µL D = Dilution factor of virus V = Volume of virus in reaction mix (4 µL) Sample Data When...Titration by qPCR AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction...Water Procedure Preparation Before handling any viruses, get materials ready. Ensure that primers, probe...contamination of the PCR reagents and the NTC with virus. Pre-warm the 96-well plate sealer by gently touching...plate and pipette 5–10 times to mix. Dilute the virus as follows: Mix dilutions thoroughly, but pipette...

...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse...Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful gene delivery ...Cell Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples...

...Background The Coronavirus Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the... elucidate as much as they can about this novel virus. It is now known that SARS-CoV-2 entry, like that...suitable for generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent... Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the size and complexity...mediating the entry of SARS-CoV and SARS-CoV-2 viruses. ( 1 ) TMPRSS2 - a serine protease that primes ...primes the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme that cleaves... to the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine...

...catalog items. Virus-associated DNA Each viral vector service request will also include virus-associated ... the original plasmid that was used to make the virus. In order for Addgene to provide viral vector services... libraries. Viral preparation options include lentivirus and AAV. Viral...Order a high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV...

...epitope derived from the P and V proteins of Simian virus 5 (SV5) (Randall et al., 1987). The epitope (GKPIPNPLLGLDST...matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked...characterization of monoclonal antibodies to simian virus 5 and their use in revealing antigenic differences...

...service as one of those stories. Viral vectors are viruses engineered to be safely used to deliver a genetic...especially those familiar with adeno-associated viruses (AAVs), will know just how tricky these tools can... Kamens, J., & Fan, M. (2019). Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...