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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 955 results for: ARC;

Showing: 591 - 600 of 955 results

  1. CRISPR Antimicrobials

    Type
    Blog Post
    ... by antibiotic resistant bacteria. Even when researchers develop new antibiotics, the onset of resistance...’re turning the tables on the bacteria. Future research will show if this new strategy can help us win... on Addgene.org Find CRISPR Plasmids for Your Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...derivatives are a staple in the lab. GFP is used in research across a vast array of biological disciplines ...protein and facilitating its widespread use in research. Many other mutations have since been introduced... Marcy Patrick...

  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...amino acid across an entire polypeptide. Many researchers have created codon-optimization algorithms/resources...they can effectively inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112...databases: status for the year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292...

  4. Viral Production at Addgene

    Type
    Blog Post
    ...of the transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the...purification kits.     Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer... plasmids used in Addgene’s viral production. Barcoding allows our DNA and virus to be monitored throughout...

  5. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...different kingdoms of life. That’s convenient for researchers who want to do experiments in complex eukaryotic...course, that includes you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even...experiments. Reporters Finally, in some cases a researcher may choose not impose selection on cells and ...

  7. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...useful for organisms, they can be problematic for researchers trying to express proteins in heterologous hosts... cost DNA synthesis, one of the primary ways researchers solve the problem of codon choice is to resynthesize...of these factors on their own. Luckily, many researchers have created codon optimization algorithms and...

  8. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...yet sometimes overlooked, aspect of scientific research is that the field benefits from collaboration ...neuroscience field is a great example of this; research benefits when the advances of some are shared ...help illuminate (is that pun worn out yet?) your research. Derek P. Simon received his PhD in Cellular ...

  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...Resources on Addgene.org Find Plasmids for Your Research     ...

  10. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ... generate full libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation...saturation mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al...Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined...
Showing: 591 - 600 of 955 results