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We narrowed to 641 results for: ato

Showing: 591 - 600 of 641 results
  1. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing...Class II, Type A2 Biological Safety Cabinet 4 °C Refrigerator Pipette controller Benchtop centrifuge compatible...tubes NanoDrop spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead...upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate filtered tissue culture supernatant...
  2. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Harvest antibody Equipment Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes...conical tubes Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead... mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip ...swirl 5–10 times to mix. Return the flask to the incubator. Section 3: BCD Feed and valproic acid supplementation...
  3. Deep Dive: qPCR

    Type
    Blog Post
    ...19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais ...
  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., & Zhang, F. (2013). Double nicking...
Showing: 591 - 600 of 641 results