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We narrowed to 955 results for: ARC;

Showing: 601 - 610 of 955 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...application for basic and translational research.  Basic research applications of scFvs: The SunTag system...Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest...

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...useful and most importantly, effective. In the research world, there is a myriad of tools this could be...intended target. RNAi is a valuable tool for your research arsenal, and there is still a dedicated field ...about miRNA-guided gene silencing. Nucleic Acids Research, 50(2), 617–634. https://doi.org/10.1093/nar/gkab1256...

  3. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...would be directly reported by band intensity. Researchers use later steps in this chain (blue) to infer...controls (green). Created with BioRender.com. Researchers often rely on assumptions that their loading ...your particular setup. We recommend starting by searching the literature to see what is already known about...

  4. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... COVID-19 pandemic. The Pandemic Pivot Back in March 2020, just four months after I started with Addgene...completely out of reach for Addgene.  So, onto research! I needed to design a system that would allow ...  Quintin Marcelino...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...efficiency of homology directed repair, with researchers hypothesizing that they can efficiently integrate...bp sequence around the desired insertion site to search for potential gRNAs. As with other homology-directed... under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently...

  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries for Your Research ... human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...mouse genome. With these early technologies, a researcher would first introduce a mutation into a mouse...and rushed to test it. I still remember the day, March 16, 2014, that I got my first preliminary results...CRISPR-Cas9 is an acquired immune system in bacteria and archaea. As you know well if you’ve been following the ...

  8. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...couldn’t be luckier with my mentor, since she does research at UK in the same field that I do: prostate cancer...highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen Vela, ...    Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where she studies...

  9. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...high levels of spurious expression.  As such, researchers developed new tools called FLEx/DIO vectors where...expression levels over several weeks allows the researcher to determine when the optimal time to conduct...merely a new lot, be thoroughly tested in the researcher's system before beginning large scale experiments...

  10. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...stability, or other properties is an active area of research. If size is a concern for your application, SNAP-tag...ligand to use for your experiment. Conclusion Researchers and vendors continue to develop new ligands for...Proteins Inside Living Cells. Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f...
Showing: 601 - 610 of 955 results