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... you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100... plates with colored marker - particular colors correspond to particular antibiotics. Position the flame...good idea of what that volume looks like in your particular plate. For the remainder of the plates, pour ...

...for PCR Based Cloning The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get... get PCR amplification. We can start similarly, taking the final 18bases of the ORF, including the stop...will need to use a phosphatase to prevent re-circularization of the vector. You should treat your digested...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ...

...high molecular weight proteins and higher percentage acrylamide when immunoblotting low molecular weight...and reagents from other vendors should produce similar outcomes when using these protocols. However, please... ideal lysis buffer will vary depending on the cellular location of the protein of interest. RIPA buffer...buffer will vary depending on the sample type and cellular location of the protein of interest. You may need...

...Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced...Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

...without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying the transdifferentiation...transdifferentiation is suited for switching cell types between similar lineages, reprogramming is more versatile and universal...PMC3390875. 25. Patsch, C., et al., Generation of vascular endothelial and smooth muscle cells from human...

... the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins. The C-terminal...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed with UCSF ChimeraX...DNA to read its sequence. Nature Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038...

... here in New England- the majority of which are solar, wind, or hydroelectric in nature. The operation... electricity will allow us to maintain these particularly energy-intensive systems in a more environmentally...cartridges can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges...

...expertise through our blog. Do you have skill in a particular technique? Do you have a set of tips you give... An overview of software tools useful to any molecular biologist Tips for protein purification Replica...Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen interested in applying CRISPR...

...fluorescent markers or protein tags, and suitable for molecular cloning. Plus, we decided to include 10 plasmids...µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments such as cloning, digestions... Learn about our other beta projects Read our Molecular Biology Reference ...

... countries. Guides and protocols were our most popular content, while our beloved blog received 1.3 million...scientists in this rapidly changing environment. In particular, we are working to understand what challenges...Addgene colleagues, collaborators, partners, and the larger scientific community to maximize the positive impact...