We narrowed to 971 results for: lars;
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TypeBlog Post...undeniably difficult connection to study on a molecular level. The brain is constantly influenced by signals...organisms, including the Anikeeva lab’s work on a similar neural fiber (Park et al., 2019). This provided...., Anikeeva, P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System for Wireless, Programmable...
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Plasmids 101: TOPO Cloning
TypeBlog Post...low concentration or you are cloning an extremely large insert. Since the standard ligation reaction is...Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...that cells with common ancestors tended to have similar reprogramming outcomes. For example, cells with...with shared CellTagD13 tags often had similar gene expression pattern changes and reprogramming outcomes...reprogramming is not stochastic, but rather there’s a particular cell state that’s permissive to reprogramming.In... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post... including more secure archiving, unambiguous molecular definition, and potential for further engineering...is a critical unmet need for an open-access, molecularly defined, and well validated collection. I’m thrilled..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...but for viral vector core facilities producing a large number of viral preparations in parallel, there ...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org...Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...interested in exploring biological dark matter – large tracts of the microbial tree of life that remained...IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans... incredible thermostability makes the enzyme particularly useful. Additional resources on the Addgene... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute...variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able ...cleavage efficiency. If this finding is replicated in larger studies, it will represent a key advantage over... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog Post...of Addgene's top blog posts from 2015 Our most popular posts continue to be those from our Plasmids 101...regardless of their end goals and believe the popularity of our career posts shows that we are doing just...Ramsden from UNC Chapel Hill explain some of the molecular gymnastics behind DNA repair by non-homologous... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post... Syndrome One of those teams is led by Claudio Aguilar (right) at Purdue University and is working to ...ago, but there is still no treatment available. Aguilar is a basic cell biologist. He got interested in...meet a few patients that suffered this disease,” Aguilar said. “Once you have that, there is no coming back... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...individuals in their 20’s who’ve built multibillion dollar tech companies using only laptops and some server...story altogether? The unfortunate answer is yes, largely due to a few limiting factors. To start, the average...different than what was originally planned, due in large part to the feedback we received from our network...