We narrowed to 961 results for: App;

1. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   Blog Post

   ...guarantee that their experiments will work (yes, this happens): Science is hard. Chris Voigt, another Addgene...every conceivable background at the meeting. I appreciated all the feedback I received from scientists, ...

2. Plasmids 101: Antibiotic Resistance Genes

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   Blog Post

   ...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline...

3. Virus Protocol - Generating Stable Cell Lines

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   Protocol

   ...expression observed using transient transfection approaches, generating cell lines using lentiviral vectors... tubes, VWR 21008-216 Lentivirus preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you.... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. This is the beginning of the selection...

4. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

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   Protocol

   ...bottom. Add 5 mL of Pierce IgG Elution Buffer to the capped Gravitrap column and incubate for 10 min at RT...subsequent centrifugation step Pro-Tip Resin will appear compacted after centrifugation. Discard the flow...column in a new 50 mL LoBind collection tube. Slowly apply the sample to the center of the compact resin bed...exchange and concentration with an Amicon column Apply 15 mL of PBS to the reservoir of a Amicon Ultra-...

5. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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   Blog Post

   ... Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established... Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. In...

6. CRISPR Challenges: Standardization and Homology Directed Repair

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   Blog Post

   ...the gRNA design process and rules for various applications is necessary going forward. The standardization...Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: Targeting RNA with Cas13a (C2c2...

7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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   Blog Post

   ...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools...

8. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   Blog Post

   ... the future, this stitching technique could be applied to other epitopes and scaffolds to build a more...antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based...

9. Single-cell tracking of lineage and identity with CellTag

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   Blog Post

   ...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...References Biddy, Brent A., et al. "Single-cell mapping of lineage and identity in direct reprogramming...

10. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post

    ...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...