We narrowed to 819 results for: cell
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TypeBlog Post...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...
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Five Popular Model Organisms, Part 2
TypeBlog Post...are often used to study vertebrate embryology and cell development. One major resource for frog research...Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...is immediately ready to transform into competent cells. The master mix of enzymes can be purchased from...mix for 1 hour, and then transform into competent cells. The success rate of this reaction is typically ... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...introduction of nucleic acids into bacteria or eukaryotic cells is a common technique in molecular biology and scientific... -
Distribution to Industry
TypeCollection...plasmids for detecting GPCR activity in a variety of cell types. Protein Purification Optimization Kit Using... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed PMID 18657511... -
Technical Design of a Western Blot
TypeBlog Post... mechanical method before your enzyme lysis. For cell cultures, enzyme lysis is typically sufficient. ...proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing lysis buffer, ...while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the proprietary...expensive Nitrocellulose or low-fluorescence PVDF membranes Increased fragility (nitrocellulose) or increased...When choosing your lysis buffer, consider the subcellular location of your protein. Proteins located in...Membranes The two membrane options are PVDF and nitrocellulose. PVDF is a sturdy membrane that can be stripped...membrane, it also has higher background noise than nitrocellulose membranes. Pro tip! You can visualize proteins... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58(5):718-21. doi: 10.1016/j.molcel.2015.04.025... -
Microbiology Resources
TypeCollection...applications like recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK... -
CRISPR Plasmids - Prime Edit
TypeCollection...and the resulting heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if...