We narrowed to 977 results for: App;

1. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   ... the appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different...

2. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ...reactions by mixing this isolated plasmid DNA with appropriate sequencing primers, and hand deliver them to ...standards are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first...

3. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...faster deamination rate so that deamination can happen before Cas homologs let go. The Liu lab developed...

4. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ...researchers (or your own from years ago!) do you really appreciate how few details are provided in most published...important enough to be answered and the methods are appropriate to answer those questions, the article is given...

5. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   Blog Post

   ...TurboID and miniTurbo podcast segment A fundamental approach to studying membranes, organelles, and other subcellular...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin...

6. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   ...product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan...

7. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...over or write to us to get them. Even though we’re happy to provide them, that never works on a large scale..., or words of advice for people who may want to apply this tagging system to their own work? Mendenhall...

8. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ...Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome. Non-homologous...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of...

9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   Blog Post

   ...design,” she said. “Before it was a little like tapping in the dark - a lot of trial and error.” The structural...analyzing the data. It should be straightforward to apply the new library for use in various cell types and...

10. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post

    ...to today, Sample6 has changed focus and names, swapped leadership, and grown to 25 members.  What is...competitions. After much learning, the focus of the application was changed, but Sample6 still leverages the ...