...necessarily expecting a perfect match - we will often find a few mismatches in the origin of replication ...sequencing, we feel confident that these few minor mismatches usually don’t affect the function of the plasmid.... We also confirm that the species of the gene matches the data associated with the plasmid.     Confirming...

..., the Roska lab built synthetic promoters from scratch and tested to see if they drove cell type specific...channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing ...Synthetic promoters did, however, tend to loosely match the specificity of their source genes, i.e. if a...drive expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only result...

...scientific poster apart? First, a good poster should catch the audience’s eye and draw people in. That can ...may choose to display it as rows. It can be more catchy because it is generally less common and will stand...advantage of being different and potentially more catchy, but it can be trickier to make figures fit nicely...relevant to the work you are presenting. The latter is catchy, but beware. A distracting background or clashing...

...interest. Every batch of AAV requires its own MOI optimization to account for batch-to-batch variability....Ultracentrifugation Protocol for more details. Pro tips Watch this AAV purification video! It didn’t exist when...

...any discrepancies in Sanger sequences, such as mismatches, deletions, or insertions. We use blastp or blastx... the species that your sequencing result should match, enter the common or scientific name into the Organism...smaller word size and lower scoring penalties for mismatches and gaps in the alignment. If you are curious...Sanger sequencing result and noted any potential mismatches or gaps in the nucleotide sequence, you can run...

...NatureJobs: Interviews: The All-Important Job Talk. 1. Watch other people give great talks We are not talking...are those in the Young Scientist Seminar Series. Watch how these speakers talk without looking at their...entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking 101 Talk 2. Tailor...of saying “so” and “um” a lot. I hate it when I watch myself speak, but others tell me that I keep the...

...either side of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers to successfully...protocol. Be sure to adjust the extension time to match the size of your template. Amplification of GC-rich...only DNA that is methylated at the adenosine of the GATC recognition site. Transformation: After the PCR ...transform your PCR products into will efficiently patch up the DNA. The resistance marker from the parental...

...fragments. As a fun way to start, I highly recommend watching this entertaining video created by our friends...want next to it, so this doesn’t allow for mix and match of many different fragments. One way around this...homology regions, which allows for the mixing and matching of parts. Gibson assembly meets CRISPR Gibson... Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene ...

...least a year when stored at -80oC.   For each new batch of competent cells, don’t forget to do a negative...the number of colonies obtained using your fresh batch of competent cells with those obtained using commercially...commercially available cells or previous batches of homemade competent cells to ensure good efficiency....

...optimized until the multiplexed and individual values match across the board. If the samples or sample conditions...need to make sure the assumptions in your dataset match the assumptions in your analysis method. ΔΔCT/CQ...’re not sure if your amplification efficiencies match, you’ll need to run a validation assay in which ...favorite (...er, whichever one uses assumptions that match your data set, that is.) Yuan, 2006 has a good overview...