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We narrowed to 469 results for: Lif

Showing: 121 - 140 of 469 results

  1. Guide to Using Pooled Libraries

    Type
    Guide
    ...see if it should be amplified before you conduct your screen. If you need to amplify the library, please... Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol. Read ...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens...: Pooled Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to... 175,000 different gRNA-containing plasmids). Amplifying and Using the Library Once you receive your library...general steps to take in the screening process. Amplify the library (electroporation and maxiprep). If ...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their cDNA...

  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...fusion proteins Biotin is an essential vitamin for life Although biotin is most commonly known for its role...essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants, most bacteria...sites found on avidin are also ideal for signal amplification during these experiments. During detection, ...bound to your protein of interest leading to amplification of the reporter signal and easier detection....

  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...by scFv antibodies fused to sfGFP. This system amplifies the intensity of fluorescence signal and enables...gene of interest. The gene to be expressed is amplified using a gene-specific forward primer containing... based on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a ...ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221 can be expressed either...(MuLE) system from the laboratory of Ian Frew simplifies the process of creating complex polycistronic...iterative, chaining-based cloning method that simplifies the process of constructing specialized fluorescent...destabilizing sequences were added to shorten the half-life of both the luciferase mRNA (3’UTR AUUUA [5 consecutive...

  4. Finding Your Perfect Job After University

    Type
    Blog Post
    ...language. During the recession there were many qualified scientists with experience and a second language...carry out my dissertation research in a lab in California. I felt then, and still feel now, that it is ... do. I had taken advanced virology classes in California and enjoyed them, so I applied for a role at ... day planned out. This allowed for a better work-life balance compared to working in research. My previous...

  5. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...biomedical scientists and had no idea what the daily life of a scientist was like. The word “scientist” conjured...those scholarships that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots... feel less confident in their abilities and qualifications. Studies on imposter syndrome have also found...and ended up with the award. We are often more qualified and capable than we know or believe we are. Funded...

  6. CRISPR Guide

    Type
    Guide
    ...experiments and must be amplified before use (Figure 8C), following the amplification protocol specified by... by the depositing lab. After amplification, you’ll need to use next-generation sequencing (NGS) to verify...verify that the composition of your amplified library matches that of the original library. Next, you’... copies of the aptamer improves stability and amplifies the signal for better imaging of genomic loci,...SHERLOCK (and SHERLOCKv2) allows for greater amplification and quantification and can be used on lateral... Innovative Genomics Institute, University of California, Berkeley. Plan Your CRISPR Experiment Resources... DNA PCR P olymerase C hain R eaction; Used to amplify a specific sequence of DNA pegRNA P rime E dit ...

  7. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...the scoop on the ups and down of graduate student life. Senior graduate students can sometimes be elusive...and talk to us and wish them the best of luck in life beyond graduate school. Ben J. Vincent is a Ph.D...fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome biology and...

  8. Reflections and Looking Ahead

    Type
    Blog Post
    ...reproducibility in biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...Request, a service that dramatically expands and simplifies your viral vector options. Almost any AAV transfer...

  10. Editor's Choice, September 2016

    Type
    Blog Post
    ...that deserve a second look. 5 Great Apps for Lab Life This very useful post was contributed by guest blogger...growth medium, these apps can help make your lab life a bit easier. Since the post doesn’t provide an ... lab life...

  11. Science as Art

    Type
    Blog Post
    ...past few years, including an article in the BBC Wildlife Magazine. It is easy to see how these incredible...information on black screens, but not to know even the life cycle of the creature they deal with.” So photography...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and art was Albert...

  12. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...bold. You don’t have to have all of the listed qualifications to apply for a position.  A job description...application). Beware the myths of scientist “overqualification” but it may not be productive to apply for...resumes The cover letter must address how you are qualified and why you are interested specifically in the...

  13. An Addgene Summer Internship

    Type
    Blog Post
    ...that I belonged there. Addgenies had a good work-life balance and were content. As a worker, there are...them. Addgene gave me the chance to become a real life scientist. Addgene gave me the chance to grow into... arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want it any other...

  14. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ..., allowing me to pinpoint areas of my career and life where I excel (and thus feel grateful) and identify...once said: “There are only two ways to live your life. One is as though nothing is a miracle. The other...quote encourages us to contemplate our approach to life and how our viewpoints influence our encounters....

  15. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...articles openly available on PubMed Central (also eLife, PLOS and Figshare). Some use cases include: Cataloging... collections from a large section of open access life sciences data. You can use ReFigure to coherently...we can only connect figures from PubMed Central, eLife, PLOS and Figshare but we are working on expanding...

  16. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS)...viral genome sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...routinely producing AAV. We believe that this simplified sequencing and analysis approach can be easily...

  17. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...which limits its use for imaging a protein’s entire life-cycle. This makes it nearly impossible for scientists...permanently attached to a protein, making them hard to amplify and image for long periods of time.  Are antibody...demonstrated that the HA frankenbody can be used to amplify fluorescent signals in in vivo imaging: the 10xHA...

  18. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...with and/or requests materials from Addgene can qualify. We also reward improvement against one’s own record...Hospital  Brandeis University  Broad Institute  California Institute of Technology Cold Spring Harbor Laboratory...University of British Columbia  University of California, Irvine University of Massachusetts, Worcester...

  19. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...researcher on parasitoid wasps; and an essay on the life of Srinivasa Ramanujan, to be precise) and remarking...neurological health risk. Having played the sport my entire life, I was curious as to what the research had to say...chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry. A research...

  20. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone...created by James Trimmer, PhD, of the University of California Davis School of Medicine. This deposit includes...
Showing: 121 - 140 of 469 results