We narrowed to 313 results for: bon;

1. How-to: 5 Steps to a Great Panel Discussion

   Type

   Blog Post

   ...before the event... Choose a moderator with a backbone. It is nice to have a subject area expert, but...

2. Cloning Mammalian Cells with the Agarose Method

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   ...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

3. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...

4. Polymerase Chain Reaction Overview and Applications

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   ...the dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the...

5. Plasmids 101: Protein Expression

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   ... include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems...

6. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic gates." Science...

7. CRISPR Plasmids - Empty gRNA Vectors

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   ...S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm EcoRI none S. pyogenes de Bono pMB60 47941 Worm... cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes Zhang pSpCas9n...none S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...BsmBI none S. pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian... cut S. pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI... SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian...AAV SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian...

8. Antibodies 101: Producing Recombinant Antibodies

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   Blog Post

   ...periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’re using a mammalian...

9. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   ...Maleimide-labeling occurs when a maleimide group forms a covalent bond with a sulfhydryl group (–SH), such as that found...

10. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...is irreversible — FP fragments become covalently bonded by associating with one another — which means it...

11. DNA Purification Without a Kit

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    Blog Post

    ...extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules and coaxes it into sticking...

12. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    .... S. cerevisiae) Plasmid Type: Choose an empty backbone if you intend to clone your own insert, or use...

13. Isolating Genomic Regions of Interest with the CRISPR System

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    ...enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed...

14. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...immediate co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the...

15. Cpf1: A New Tool for CRISPR Genome Editing

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    ...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

16. Plasmids 101: Protein tags

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    ...such as Thioredoxin A (TrxA) that improve disulfide bond formation in order to help keep your protein soluble...

17. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method ...

18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...Click here to view Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription...

19. Using Video to Share Your Science: We Share Science

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    Blog Post

    ...rather than on the day-to-day research that is the backbone of science. Facebook is continuing its growth,...

20. Antibodies 101: Immunoprecipitation

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    ...differing amounts of target proteins or antibody bonding strengths.    . Steps of an IP: 1) Protein...

21. Viral Vectors 101: Pseudotyping

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    ... type, but other envelopes such as the one from gibbon ape leukemia virus (GALV) are more stable (Sandrin...

22. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...oligos are required and the BsaI linearized pEcgRNA backbone can be prepared in large amounts and frozen for...

23. Plasmids 101: Stringent Regulation of Replication

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    ...BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together, it recruits helicases ...

24. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    ...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination...

25. Finding nucleic acids with SHERLOCK and DETECTR

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    ...O, Jinek M (2016) Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated...

26. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    ...interesting behaviors. There is diversity in the social bonds that they form including genetic mechanisms that...

27. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...toxin-antitoxin system is MazE/MazF. The toxin MazF is a ribonuclease that cleaves specific sequences in RNA resulting...

28. Viral Production at Addgene

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    ...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

29. Viral Vectors 101: Preparing Pooled Libraries

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    ...with a protocol for a library of similar size and backbone.  Verification Once your library has been amplified...

30. Plasmids 101: Shuttle Vectors

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    ...Resources on Addgene.org Collection of empty plasmid backbones ...