Hot Plasmids - February 2022
Type
Blog Post
...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021.
Anti-FLAG and anti-HA...
We narrowed to 345 results for: ada
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Antibodies 101: Isotypes
TypeBlog Post... constant domains. This switching helps B cells adapt during the course of an immune response since it... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...’re not at your computer. Maps, sequences, & downloadable .gb & .dna files Once you’ve identified a plasmid... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al. You can also search by ... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...that can make vector propagation and sequencing a headache. How do I maintain ITR integrity and prevent unwanted... -
Viral Vectors 101: Biosensors
TypeBlog Post...measured GFP levels and converted that signal into a readable value. Types of biosensors So now that we know... -
Viral Vectors 101: Viral Applications
TypeBlog Post...which target complementary mRNA sequence for degradation. Viral introduction of shRNA plasmids can induce... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell ... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...10.1093/ilar.46.3.258 Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post... dsRNAs target specific mRNA transcripts for degradation. Despite how easy these experiments are to physically... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post...in science communication from across the US and Canada convene at ComSciCon's annual flagship workshop... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...RspWYL1 increases both targeted and collateral RNA degradation by RspCas13d. RspWYL1 also increased EsCas13d... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...Repression and Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...ardor and attended to with diligence.” - Abigail Adams, 1780 There are potential mentors all around you... -
Click Biology: How to Click and Play to Explore Biology
TypeBlog Post...fluorescence or triggering new cellular behavior like degradation or electrophile signaling. Many of these tools... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...provided by the major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but... -
Summer SciComm: Preprints
TypeBlog Post...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla... -
All in a Twist: dsRNA
TypeBlog Post...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...cells promote oxidative stress leading to the degradation of one key enzyme (among others): caveolin 1,... -
Visualizing Protein Turnover In Situ
TypeBlog Post...the combined effect of protein synthesis and degradation. These processes control the abundance of proteins... -
Plasmids 101: Cre-lox
TypeBlog Post...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021. Let me leave... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your own citizen... -
Hot Plasmids February 2024
TypeBlog Post...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission. Find... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...triggers a cascade of events that results in the degradation of the target mRNA and silences its expression... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...Office Ben Corb - Director of Public Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...Project One such space is the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...colocalization analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...