...the supplement of this paper), help each person organize his or her thoughts to facilitate honest conversation...for their time in the lab and their next steps. Addgene would love to hear about the steps taken in your...

...reagent type, target species, target name, host organism, isotype, clone details, vendor, method, tissue.../10.1073/pnas.2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype...

... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration...interaction, thereby changing the FP’s structural organization and activating the fluorescent signal. Blue ...fluorescent molecule   Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...extracellular molecule? By fusing a biosensor to an organelle-specific targeting signal or a transmembrane domain...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

...perfect job.   Resources at Addgene Read Additional Career Posts on the Addgene Blog Browse Our Careers ...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...applied anyway. I later found out the job was with Addgene. I had used plasmids from this company before and...also get the chance to contribute content to the Addgene’s Website and travel to meet scientists, making ...develop my own projects, but working remotely (Addgene’s main offices are in Cambridge MA, while I’m based...Careers Page to See What Positions Are Open at Addgene Learn How to Choose a Good Mentor for Your Professional...

...deposited these plasmids at Addgene. While deposits from for profit organizations are less common, they can...This year, Addgene welcomes three new members to the Board of Directors: Khandan Baradaran, Frank Stegmeier...assay technologies makes her a perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics... the research funded,” she says. Baradaran met Addgene Executive Director Joanne Kamens several years ...through this connection that she learned more about Addgene. “The mission to share critical reagents such as...Scientific Officer at KSQ Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically...finds the value in the wealth of resources on Addgene’s website and blog important. “The experience from...

...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...Simon Singh's "The Code Book" for this month's Addgene Book Club. I read most of the book on my way to...from those who expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious...this meeting. And of course, I want to thank the organizers of this meeting who have given the attendees ...increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing the poster...

... biology tools.  Addgene Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors...you keep your virtual molecular biology toolbox organized, today’s post features a list of free online molecular.../ protein database search and analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs...restriction enzyme cut sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled...available sources, as well as those deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences...tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence manipulation... Rachel Leeson.  Additional resources on the Addgene blog: Learn how the CRISPR software matchmaker...

...guest writers. Your contributions make Addgene and the Addgene Blog useful resources for the biological...It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by ...highlighting some of the great posts written by Addgene depositors. Below you’ll find brief summaries of...depositors. Interested in writing your own post for the Addgene Blog? Fill out our guest blogger form to get started... section of DNA into the genome of your target organism. In this post, Chris Richardson, a postdoctoral.... Note: Casilio is no longer available through Addgene.  Thanks again to all of our fantastic depositors... addgene...

... rigorous research plans. However, amidst the organized chaos of lab research and PhD studies, there lies...and execution plans.    More resources on the Addgene blog Experimenting with New Careers While in Grad...

... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ...peering right through living animals to see their organs – a liver or brain, say, or maybe a tumor. They...for optical labeling and tracking of proteins, organelles, and living cells in a spatiotemporal manner,...  Kendall Morgan...

...FAQs, Le Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular...full list of 16 FAQs answered by Le Cong Read Addgene's CRISPR guide for background information on CRISPR...the most frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools: List of...scientific community and sharing these resources with Addgene. Thank you, Le!    ...

... What is an Antibody? Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using...different research applications they can be used for. Addgene’s Antibody Plasmid Collection includes plasmids ...10.3389/fcimb.2013.00072 Additional resources on the Addgene blog: Read about another reagent sharing platform...

...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...Medical Art This site has 3,000 free medical images organized into four main categories: anatomy and the human...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...

... about the role these factors played within an organism.  So how did plasmids get their name? In 1952...diversely applied to many different experiments. Addgene was founded in order to store, QC, curate, and ...cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook And now for some ..... You can now download the newest version of Addgene's Plasmids 101 eBook! In keeping with our goal of...

...skills guide Resources on Addgene.org Learn more about Careers at Addgene Watch out career videos   ...skilled at presenting and answering questions. Organizing a thoughtful engaging talk takes significant ...successful writers about their strategies for organizing and self-critiquing their own writings. 8. Learn...outdoor photographer.  Additional Resources on the Addgene Blog Find Tips for getting a faculty position ...

... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...that mythical place where antibodies are well-organized, documented, and easy to find (though we might... References and Resources More resources on Addgene’s blog Antibodies 101: Monoclonal Antibodies Antibodies...

... will make specific, controlled changes to an organism’s genome (genome engineering) Produce synthetic...that can be used in research or for therapeutics Addgene has compiled various educational resources to facilitate... facilitate plasmid use in the lab. Addgene’s Molecular Biology Reference includes information about molecular...

...Searching, Reading, & Organizing Write for the Addgene Blog Resources on Addgene.org Check out Our Revamped...100percentSDS.     Additional Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific...

...study single cell dynamics in model organisms Resources on Addgene.org Visit our stem cell page Find plasmids...CellTag, you can find the CellTag libraries at Addgene. The Morris Lab's website also has several useful...bioRxiv (2019): 335547. Additional resources on the Addgene blog Learn more about induced pluripotent stems...

...URL “http://n2t.net/addgene:87155” where the Addgene registered prefix and the Addgene ID # are directly...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155).” So when citing...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155)” Cite plasmid...through Addgene and find verified information about each plasmid item. Given a specific Addgene ID # listed...available through Addgene. Let’s take a look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The...contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene. How to...was a gift from Daniel Bauer (Addgene plasmid # 87155 http://n2t.net/addgene:87155)” Cite plasmid using ...

... Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter their...hunting and interviews, I accepted a position at Addgene as a marketing and social media co-op. There are...realized I had made the right decision to choose Addgene for my co-op, but I know it didn’t take long. It... It may have been at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached...analytics, something that had never been done at Addgene before. This meant I was responsible for establishing...understand what metrics would be useful and how to organize the metrics in a digestible manner.  At first,...strategy. I really believe that the culture at Addgene cultivates trust between coworkers and that trust...

... that mimic aspects of organ morphology and physiology in vitro and have emerged as a powerful model for...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... CRISPR-based genome editing in organoids By Ashley Waldron Organoids are three-dimensional cultures that...toolkit for performing genetics studies in organoids. The Organoid EasyTag System is a straightforward workflow...in an organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag...fetal-lung derived organoid system, the approach is intended to be amenable to organoids derived from other...find in this article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR...

... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict...10.1089/hgtb.2019.276 Additional resources on the Addgene blog Learn more about basic viral vector elements...cell-type expression in retinal cells Resources on Addgene.org Find AAVs for your experiment Browse our AAV...

...transposons can be used to both introduce genes into an organism’s genome (see figure) and to disrupt endogenous...10.1111/imr.12137. PubMed. Find the Plasmids at Addgene Kowarz Lab plasmids Izsvak Lab plasmids  ...

...understand the part they play in the goals of the organization and feel the importance of their contributions...Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series as it is published...valued This often stems from being included in organizational decision-making. It is imperative that employees...

...where they are. 8)   Look into training from an organization such as COMPASS. Although COMPASS has a special...programs at both MIT and Boston University. At Addgene we're committed to scientific sharing – both your... Kendall Morgan...

...capability of transforming the genetic code of living organisms and its societal implications even before the ...pneumoniae bacteria could be transformed from one organism to the other. This is now famed as the Griffith...at @kartik_lakshmi. Additional resources on the Addgene blog Find more blog posts about CRISPR Learn more...about other genome editing methods Resources on Addgene.org Visit our CRISPR guide Find CRISPR plasmids ...

...out full plasmid sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered...annotation gaps in the plasmids available through the Addgene website. In addition, two initiatives are in the...is to enhance Collections so that a group or organization can annotate their sequences from a shared database...University of Chicago. He was an early depositor at Addgene, and conceived SnapGene because he needed it for...post-launch iterations.     Additional Resources on the Addgene Blog Read about our SnapGene powered plasmid maps...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...

...Twitch calcium sensors, which are available at Addgene, can be used for ratiometric in vivo imaging. Griesbeck...and zebrafish – essentially all of the standard organisms for which extensive genetic tools are available...the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx said. “For...(PubMed) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight...some of the popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging... Kendall Morgan...

...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...the plasmid based on the predicted sizes and organization of different features within the plasmid. Restriction...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

...multi-color imaging. Find the HA frankenbody tool-kit at Addgene to get started with your experiments! What else... frankenbody can be used for imaging in whole organisms. The researchers used the HA frankenbody to successfully...PMCID: PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover ...fluorescent protein blog posts Resources on Addgene.org Read our fluorescent protein guide Find plasmids...

...that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in...excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various important considerations...github @tlambert03.   Additional Resources on the Addgene Blog Fluorescence Microscopy Techniques - Which...Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent protein plasmids Find...

...Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone...Pam_Hines and LinkedIn. Additional Resources on the Addgene Blog What Do I Do Now? Academic v. Non-Academic...

...plasmids from this publication at Addgene! Additional Resources on the Addgene Blog Find planarian tools for...without the need to handle a living, replicating organism. Furthermore, cell free systems can be freeze-...center brings plant science to life Resources on Addgene.org Browse our protocol videos to learn simple lab...

...All organisms share an innate goal to survive. This past year, scientists hijacked survival tactics of...innovative biological and translative technologies. Addgene has empowered researchers to harness previous experimental...For more information on CRISPR/Cas, check out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your...

...expand Addgene’s services. There was an overwhelmingly positive response to the option of Addgene providing...allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing... would like to thank some of the people and organizations who helped us reach this milestone. Viral service... capabilities to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service ...build a completely self-supporting service so that Addgene is not dependent on grants. Aiming to operate under...

...help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce ... is what got him involved with Addgene - he says he’s “known of Addgene basically since the beginning ...ethic that Addgene has behind it is something that very much resonates with me.” About Addgene’s role in...Doench says, Addgene is “a central resource for what is worth having. The work that [Addgene’s] outreach ...do and to organize it and catalogue it, I mean that’s the critical function really…. Addgene’s role as ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

... to our viral vector pages on the Addgene website and on the Addgene blog to get some ideas for future...Neuroengineering Primer on Optogenetics Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse... Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...you can apply it in neuroscience. AAV tools from Addgene are already having an impact on the field Not to...horn too much, but many people who came to the Addgene booth (there were over 400 who signed up for our...virus (AAV) and lentivirus are now available from Addgene. While super useful, these vectors require a bit...

...on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding... scientists globally, Addgene has partnered with various community organizations and workforce projects...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for...Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to ...to these researchers. In the third round of the Addgene-Seeding Labs plasmid grant we are excited to introduce...its position in the river back to the user.   At Addgene we're happy to be able to support diverse groups...

...’t. Try out the new Addgene search engine! Additional resources from the Addgene blog Learn about other...other features on Addgene's website Find a step-by-step guide to using Addgene's new search Read to learn... the different website navigation paths, the organization and completeness of our information, and the...posts or reagent items.  The technology behind Addgene’s new search  Like millions of other websites, we...data in particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...effort and cross-team collaboration (thanks to all Addgenies involved). The new homepage and navigational elements...