...service with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAV1, AAV5 Optogenetics Yizhar New viral ...

... advantage of the customizability, choosing the cargo, serotype, and prep size they need for their experiments...Jordane Dimidschstein New serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral ...

...significant barriers to scientific discovery and career development. They are working with limited resources...with donors to supply surplus lab equipment to carefully-screened research and teaching departments at ...

...Marcy Patrick CRISPR-Cas9 FAQs Answered! By Caroline LaManna Plasmids 101: What is a plasmid? By...  Caroline LaManna...

...about picking a good mentor, and begin considering career options early. Tune in for great advice on all ...Looks to Improve the Research Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab ...

...about the variety of careers available to scientists on Addgene’s Science Careers blog topic. Download...

... Addgene Outreach Scientist and fellow blogger Caroline LaManna put it well: "Wow, custom mutations in...CRISPR news was circulating. Scientists at North Carolina State, in the labs of Chase L. Beisel and Rodolphe...

...for Regenerative Medicine. Darrell describes his career goals and what drives him as a praticing physician...Laboratory Medicine. He attends in the Medical Intensive Care Unit, the Pulmonary Consultation Service, the Alpha...

...not working so that I’m available to spend time caring for my mother. For fun, I enjoy pilates, jewelry...other members of Addgene's Board of Directors Find careers at Addgene ...

...with a blog post with creative ways to continue career development virtually. 6. Quantifying DNA? Here...for extracting DNA without a kit too. 1. Early Career Researcher Toolbox: Free Online Molecular Biology...

... to try this at home in their own lab: be very careful with the type IIS restriction endonuclease of choice... PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision Cloning...

...covered in a future post in this series). Members carry on back-channel or side conversations within the...and suggestions. Consider offering rewards (gift card, afternoon off, etc.) Act on information to make...

...plasmids each carrying a single gene with a defined mutation or gene fusion. Plasmids carrying the corresponding...

...genomic loci of interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector...sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...

...Essen                      University of North Carolina at Chapel Hill University of Illinois Chicago                     ...Consortium collection enables research on solute carriers (SLCs) for medical research Recently, the Michael...

... that your request is ready to order. An “Add to Cart” button will appear with the request in your Addgene... be charged once you have added the prep to your cart and checked out.  Prices for Packaged on Request...

..., R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating that ...and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing the toxicity...

...dendritic cells. These genetic tools were pivotal in carrying out my studies, and getting them from collaborators...has since studied and worked around the world, carrying out her undergraduate work at the University of...

.... Writing is the cornerstone of any scientist’s career. We use writing to communicate our findings and...consisted of graduate students, post-grads, and early career scientists. Over the course of the class, the moderator...

...data found in full-length publications. As early career researchers, we wondered how we could make the ... blogger Girija Goyal! Girija Goyal is an early career researcher and cofounded ReFigure with Joe Akin...

... stone into the next professional stage of your career by connecting you with people of similar interests... research question that people (especially you!) care about. Have you critically evaluated your plan...

...wall synthesis Bactericidal 100-200 ug/mL Carbenicillin beta-lactam Inhibits cell wall synthesis Bactericidal...optimal efficiency. Beware of satellite colonies! Carbenicillin is more stable than Ampicillin and can be used...

...Author: James Atmos (2). Many CRISPR/Cas moieties carry out these functions using slightly different Cas... Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. 2013 Jan;  ...

...Bachle  Listen to the Caspex podcast segment Steven Carr’s lab at the Broad Institute focuses on proteomics... to the C-BERST podcast segment Similarly to the Carr lab, Erik Sontheimer and his group from the University...

... MBP tag can be removed using Xa protease. The carboxyl-terminus of the fusion protein includes an AviTag...for affinity purification (see plasmid map and cartoon below). Including biotin in your culture and inducing...

...pigments primarily found in plants in the order Caryophyllales, such as cacti and beets. Betanin belongs to... Biology Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy...

...LTRs). After recombination, one resulting plasmid carries the transgene of interest (aka the viral genome...picking the smaller colonies which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed...

... new particles. As a bonus, this increases the carrying capacity of the viral vector, increasing both ... possible, just to increase the viral vector’s carrying capacity. In order to allow the viral vectors ...

...Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth ...Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAVrg Optogenetics Ofer Yizhar New serotype ...

...Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now... below.) After self-excision, the resulting loxP scar is located in this synthetic intron, and only the...

...compromising on the relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science ...Story by Angela Saini "This book is a smart and carefully presented case for better more diverse science...

...have a woman carry an edited embryo to term. (We should remember that the woman carrying these embryos...

...cell on their way to the nucleus where their gene cargoes can be expressed. The bottom line is that a lot...particles do not remain adhered to the membrane. Be careful when diluting - if you end up concentrating your...

...larger. And since the library contains many plasmids, care must be taken to ensure that all of these plasmids...powerful research tool, but their proper use requires careful amplification and maintenance of good representation...

...decolorized as gram negative. A German scientist, Carl Weigert, later expanded on Gram’s discovery, adding...resources.   Bruckner, M. Gram Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html...

...institute where their typical workdays will be carried out until the end of the appointment. The visiting...don’t forget to have fun! Visiting is an amazing career opportunity, but also a really good time! If it...

...Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol,...meaning it can utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K....

...encapsulated proteins. Video by ACS. For a more cartoony explanation of R-bodies, check out this video....Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/acssynbio.5b00237...

...intensity and/or duration. Both of these strategies carry the risk of off-target effects. Harald Janovjak ...of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles...lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing the set of plasmids required to ...

...well, so make sure you’re taking time to do them carefully and thoroughly. Have a physical copy on hand as... to Getting Started in Undergrad Research Early Career Researcher Toolbox  8 Tactics for Choosing a Lab...

...regions of the brain. However, the Cre-dependent AAV cargo would only become activated in the presence of Cre...design and interpretation of results. Take extra care when evaluating how this serotype works in your ...

... be assembled in a unique order with no cloning scars. The Elledge Lab showed that a five-piece assembly...researchers doing large amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility...

...the other hand, mScarlet-I shows correct ER labeling, which is good evidence that mScarlet-I acts as a monomer...

... disease. “My mentors Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in ...is abnormally deregulated in TS and can lead to cardiac developmental defects. Eight genes from the X chromosome...

...PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid, in an approach called Selective...Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering...

...early career scientists using fluorescent proteins in their research. These awards help early career researchers...

...ago (Field et al., 1988). Thus, we injected AAVs carrying hM4Di-HA in the brains of mice and monkeys. Surprisingly...But our data does highlight the importance of a careful characterization of transgene expression. Which...

...clearly and strategically is powerful in almost any career. It allows you to share and build support for your...with two single-panel figures, one of which was a cartoon of the protein.) In the middle of my presentation...

...used. One thing is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow their growth. Something...things like butanol - very simple molecules - a few carbons and oxygens. Nothing too much more sophisticated...

..., including hypertension, cancer, diabetes, cardiovascular disease, cholesterol gallstone disease, senescent...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...