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Showing: 301 - 350 of 535 results

  3. Synthetic Biology - Overview

    Type
    Collection
    ...International Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository of community-created...

  4. Microbiology Resources

    Type
    Collection
    ...Leishmania sp. Plasmodium sp. Toxoplasma gondii Trypanosoma sp. Plasmids for Viruses Species Dengue Hepatitis...

  5. Open Enzyme Collection

    Type
    Collection
    ... synthetic biology, DNA origami, immunology, biochemistry, and more. The foundational technologies enabled...

  7. CRISPR Guide

    Type
    Collection
    ...associated with the locus can be identified by mass spectrometry (proteins), RNA-sequencing (RNAs), and NGS (... & Liu, D. R. (2018). Base editing: precision chemistry on the genome and transcriptome of living cells...

  8. Antibody Guide

    Type
    Guide
    ...select a new antibody using the above process and try again. In some cases, it may be preferable to buy...Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting - used to...immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use labeled antibodies... one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can refer to both...both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (also called dyes...Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows you...capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the applications common...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...rtTA3 Wei Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter, for Tet-Off...

  10. Brain Initiative Collection

    Type
    Collection
    ...dendrites under the control of internal ribosome entry sequence in a viral vector Hillel Adesnik 111066...

  11. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Opti-MEM, Thermo Fisher 31985-070 0.05% Trypsin/EDTA (e.g. TrypLE, Thermo Fisher, 12605010) 1X PBS pH 7.4...Aspirate PBS and add 2 mL of 0.05% Trypsin/EDTA. Wait ~2 min. Neutralize trypsin by adding 10 mL of DMEM Complete...new vial of cells after 30 passages. Procedure Trypsinize and resuspend the HEK293T cells from 2 x T-175...cells in 1 CS2. Return to incubator for 48 h. Trypsinize and resuspend cells from the CS2. Cells should...60 mL PBS. Aspirate PBS and add 35 mL of 0.05% Trypsin/EDTA. Wait ~2-3 minutes for cells to detach. Gently...until no live cells can be seen when stained with Trypan Blue. Pellet cell debris by centrifugation at 3900...

  12. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher 12605-010) 6-well tissue ... Isolate cells from the stable cell pool by trypsinization and break up any cell clumps into individual...expanded but before they become over-confluent, trypsinize the cells and expand them to larger culture dishes...the independent monoclonal lines. Pro-Tip When trypsinizing cells from the 96-well plate, use a microscope...detached from the plate and can be isolated. When trypsinized, detached cells will exhibit a rounded morphology...

  13. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...containing the trypan blue. Pipette 10 times to mix. Load 10 µL of the cell suspension/trypan blue mix into....45 µm PES filtering system, 500 mL, VWR 430770 Trypan Blue, Thermo Fisher T10282 Valproic acid sodium...flask 5–10 times before sampling. Transfer 10 µL of trypan blue into a clean microcentrifuge tube. Vortex ...

  14. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...indicating the breakdown of ancestry associated dependencies across ancestry groups (right).    Seeking...divided into those of African ancestry(orange) and those of other ancestry groups (grey). Misek and... representation of every ancestry group, they should have found more ancestry-associated dependencies ...any other ancestry group. With the existing methodology, they estimate that the African ancestry-associated...Institute, where an exploration into the effects of ancestry and germline sequence variation in cancer cell...down unexpected paths. Though this research uses ancestry groups rather than self-reported race, it highlights...screens in DepMap to see if they could identify the ancestry of the cell lines (European, East Asian, Indigenous...

  15. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... to travel meant the accessibility varied country to country.   Figure 1: Number of countries Addgene ...Views from a country are represented in blue; darker blue indicates more views from that country.   While ...Views from a country are represented in blue; darker blue indicates more views from that country. Stats and...the USA, but our materials are available to any country that we can export to — so far, we’ve shipped materials...distributors Distributor Partnership Start Date Country Served Beijing Zhongyuan 2012 China ...distributors broadly helped increase access in the country, and we can see a growth in material distribution...distributor helped solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive...

  16. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...transferred to an industry position. If you cannot get an Industry H-1B, remember that industry career paths...Subject Industry H1B Academic H1B National Interest Waiver EB-1A EB-1B Cap subject industry H-1B There...available to you including careers in academia, industry, non-profit, and government sectors.  Within these...area, then the research role could be the point of entry into that field. In this post, we discuss the following...endeavor including but not limited to biotechnology, chemistry, architecture, engineering, mathematics, physical...-international focus. Candidates in need of an industry H-1B need to be aware of the April 1 deadline ...start working in October of the same year. The Industry H-1B visa is not the same as an academic H-1B ...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ...affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol, VWR, ...each well. Detach cells by incubating with 200 µL TrypLE for 1–2 min. Resuspend cells in 500 µL DMEM complete...

  18. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...hybridization histochemistry: use for mRNA detection.” The Journal of Histochemistry and Cytochemistry : Official...interaction has also been adapted for use in immunohistochemistry and antibody labelling. In these experiments...referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information on ABC and IHC...labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In ...Attachment to Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology of Biotin” J. Nutr, 129...Official Journal of the Histochemistry Society, 41.12 (1993): 1751–1754.PubMed PMID: 8245422. Additional ...

  19. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...study protein-protein interactions. Flow cytometry Flow cytometry is used to detect proteins on the surface...used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could be cells or ...investigate in each panel. An advantage of flow cytometry is the ability to use multiple fluorescent signals...compare between samples. Learn more about flow cytometry. Cell and tissue labeling Antibodies can be used...within the cell or tissue as well. Similar to flow cytometry, you’ll use antibodies to stain for markers of...can be analyzed using techniques such as flow cytometry. Conclusion Whew! Now you know that antibodies...

  20. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...shared his flow cytometry expertise in three posts. An Introduction to Gating in Flow Cytometry, Designing...shared her flow cytometry expertise in two posts. Flow Compensation and Flow Cytometry Controls Priyamvada... guide to understanding your flow data. Flow Cytometry Readouts Toj, a summer intern who shared their...validation expertise. Antibody Validation for Flow Cytometry Rob Hurt, whose expertise and suggestions were...

  21. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...schools to promote chemistry degrees and provide advice to students who want to study chemistry. The society... scientists are introduced to the biology and chemistry societies that will form the core of their scientific...with the goal of facilitating networking between chemistry students, especially those studying to earn a ...events throughout the year, including a Christmas Chemistry symposium and a career night. The society also...students in society and to support iGEM alumni-industry networking to promote the creation of start ups...

  22. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...while flow cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry...signaling molecule has a weak signal. For flow cytometry, secondary antibodies may be indicated on occasion...assumption is incorrect. With the exception of flow cytometry, most immunoimaging assays do not allow for absolute...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals...

  23. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...However, internationalization, the ability to cross country boundaries for both professional and personal development...boundaries and should not be confined by birth country or financial means. Instead, talent should be realized...talented professionals (who migrated from their country some years ago looking for their career improvement...bright students that will be the future of the country. Brain drain is a serious problem in several countries...immediate frontiers. Moreover, sharing a culture/country of origin with an international mentor creates ...University of Navarra, and the University of the Basque Country. Mentees represented a diverse group of individuals...

  24. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...and even some you’ve likely never heard of. Each entry displays the following information for a Cas enzyme...display both the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently... 2 – List of resources and properties for a Cas entry (SpyCas9a) from CasPEDIA.   How can I use it...RefSeq ID, etc.) and you will see a table of protein entry results. For the advanced search options, this is...System for Class 2 CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology. https:/...

  25. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...an important role in viral entry, which are inhibitors of SARS-CoV-2 entry and become upregulated during...plasmids, with many available to scientists in industry. Since March 2020, we have received over 13,000... which human genes are important for SARS-CoV-2 entry into human lung epithelial cells (Biering et al....glad to join these efforts and help academic and industry scientists through plasmid sharing and more. As...

  26. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...the most involvement with Step 1, and, while data entry can be a chore, we’ve made it easy to send us your...information in a deposit spreadsheet. The data entry process On the Addgene home page, if you scroll ...top of the page, you’ll see two options for data entry: “Submit Online Deposit” and “Download Deposit Spreadsheet...blog post for a thorough guide to our online data entry process. Figure 1: Begin the deposit process by...deposit spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries...

  27. MXS Chaining

    Type
    Blog Post
    ...create plasmids for fluorescence imaging or flow cytometry applications in mammalian cells. The modules used... various constructs with applications in flow cytometry approaches in mammalian cell culture systems (...deoxyribonucleic acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780...Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015...deoxyribonucleic acids. The Journal of biological chemistry. 1962. June;237:1961–1967. Pubmed PMID: 13918810...

  28. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ... contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do ...guest blogger, Greg Lohman! Greg Lohman is a biochemistry researcher at New England Biolabs, specializing...progress and prospects." Journal of Biological Chemistry 284.26 (2009): 17365-17369. PubMed PMID: 19329793...ligase reaction and bead enrichment." Analytical biochemistry 434.1 (2013): 34-38. PubMed PMID: 23142428. ...of low-abundance point mutations." Analytical chemistry 86.1 (2013): 900-906. PubMed PMID: 24328157. 21...

  29. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...application — flow cytometry, western blot, immunohistochemistry, immunocytochemistry, ELISA — but in all...antibody data report, showing the results of an immunocytochemistry experiment performed with the anti-Lhx6.1...

  30. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ... PhDs Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple... seminars, such as those in bioinformatics or chemistry, even if your primary focus lies within a biological...successful research career, whether within academia or industry. Albert Einstein once said: “There are only two...

  31. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...Fighting with antibodies to produce immunohistochemistry images that are crisp, bright, and lacking in...mouse-on mouse” (or any “species on species”) immunochemistry (IHC) applications an issue? The culprits are...avoiding background secondary binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your...impact other techniques like Western blots or immunocytochemistry (ICC). Not all of the solutions described...

  32. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ... PhDs Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple... PhDs face when transitioning from academia to industry is whether to stay hands-on in the lab or pivot...exploring alternative career trajectories within the industry, but the transition wasn’t easy - I had to reinvent...

  33. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...am heading into my third year of college as a biochemistry major and a Spanish minor, it’s time for me ...is a more emphasis today about non-academia and industry jobs. Since there are a variety of different career...the Addgene blog. A few of my friends in the Biochemistry major seem to have the idea that if you don’...of Business Development, got her PhD in Plant Biochemistry in France. When she moved to the U.S., she discovered...

  34. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...validation include western blot, immunohistochemistry, and immunocytochemistry. It’s important to note that...compatible with your conjugate and conjugation chemistry.  Quantification Once you’ve collected your antibody...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ...

  35. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...used rAAV FLEx-based vectors to study the neural circuitry behind the mechanisms of sleep and anesthesia....Cre recombinase protein." Journal of Biological Chemistry 259.3 (1984): 1509-1514. PubMed PMID: 6319400....behaviors: a critical analysis." Pharmacology Biochemistry and Behavior 122 (2014): 144-157. PubMed PMID... and approaches for optogenetics." Biological psychiatry 71.12 (2012): 1033-1038. PubMed PMID: 22480664...

  37. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...a desired component are cloned into Golden Gate entry vectors via traditional restriction enzyme cloning...cloning, or annealing of oligonucleotides. These entry vectors are used in a Golden Gate cloning step to...to generate the final construct. Up to eight entry vectors can be used for each Multisite Gateway compatible... Production group at Max-Planck Institute of Biochemistry has developed a number of expression vectors..., and plant resistance cassette) in pUC19 based entry vectors, as well as the pGreen-IIS based destination...

  39. Meet the New Addgene Board Members!

    Type
    Blog Post
    ... science began in high school. “I had a great chemistry teacher in 10th grade and then fell in love with...do,” Stegmeier says He began this journey in biochemistry made the foray into genetics during graduate... efforts in gene therapy either through direct industry collaborations, grants, or setting up commercial...

  40. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ... like serotype, promoter, or tool type and each entry contains an image along with key experimental details...to input your data for sharing on the site. The entry form has 5 general question types and it takes approximately...use GCaMP expression? Direct Fluorescence? Immunohistochemistry? Did you use any relevant animal treatment...

  41. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...student hasn’t been asked the question: “Academia or industry?” Once academia was the clear answer with a well-defined...uses it to match qualified scientists to jobs in industry, academia, and government. This great resource... more information Follow people in academia, industry, and government on social media (Twitter, Facebook...

  42. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015). Nevertheless, the use of these...visualizing motor, visual, and auditory neuronal circuitry in vivo. A variant of RABV has been developed ... allowed for detailed mapping of the neuronal circuitry involved in learning and memory (Kohara et al....PMID: 21825165. PubMed Central PMCID: PMC3174680. Buttry, J.L., and Goshgarian, H.G. (2015). WGA-Alexa Transsynaptic...

  43. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...diverse array of processes. The food processing industry, for instance, employs psychrophilic enzymes for...extremely useful benefits for any food processing industry as it can potentially help minimize the incidental...iGEM teams to interact with personnel from the industry and brainstorm with each other. The event also...

  44. Antibodies 101: Isotypes

    Type
    Blog Post
    ...applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting...

  45. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...unique regions as well, though. Viral packaging and entry The genetic cargo requires viral packaging to infiltrate... from the transfer plasmid. Viral packaging and entry The viral packaging proteins for AAV are rep and...replication of the dsDNA virus. Viral packaging and entry Adenovirus is quite large and has more helper plasmid...

  46. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...industries tend to hire their former interns to fill entry-level positions right out of college. Prior to March...also serve you well when you are applying for an entry-level position following graduation. While these...for internships, scholarships, fellowships, and entry-level positions post graduation. Many thanks to...

  48. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...visualization of specific proteins in situ immunocytochemistry is perhaps the most common approach. With...students at the Max Planck Institute for Biophysical Chemistry, Göttingen in Germany. I attended this symposium... labeled proteins can be detected using click-chemistry that covalently links the azido containing AHA...

  49. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ... of transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist...We Turn Academics into Confident and Successful Industry Professionals”. This larger shop might appeal ...excellent in coaching for communication skills. Industry transition is also an area of specialty for Michelle...

  50. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’... (i.e. RNA replication with no proteins). The chemistry is complex and this work requires an interdisciplinary...forms as we shared millions of years of common ancestry with worms before evolutionary divergence. Nikolaus...
Showing: 301 - 350 of 535 results