We narrowed to 200 results for: ADAP

1. Hot Plasmids - February 2022

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   ...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...

2. Antibodies 101: Isotypes

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   ... constant domains. This switching helps B cells adapt during the course of an immune response since it...

3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes...

4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...

5. CasPEDIA: A Functional Classification of Cas Enzymes

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   ...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al.   You can also search by ...

6. Luminescent Imaging with Nano-lanterns

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   ...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-...

7. A Deep Dive into BioBuilder

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   ...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current...

8. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   ...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4...

9. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   ...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...

10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...

11. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence...

12. Plasmids 101: The protein expression toolbox

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    ... Fig. 3 dTag mechanism of protein degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral...

13. Plasmids 101: Cre-lox

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    ...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...

14. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...

15. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

16. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...

17. Adenoviral Vector Production and Troubleshooting

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    ...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...

18. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to work in your lab. Find...

19. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

20. Open Resources and Plasmid Tools For Studying C. elegans

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    ...Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered in plants to conditionally...