We narrowed to 987 results for: Abo

...Addgene lists the primers used to obtain each result above the posted sequence in the "View Sequence" link....sequence than expected and wish to contact Addgene about the accuracy of your plasmid, please email help@...

... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to ...even if you are using disinfectants as described above in step 3. You will also need to maintain the appropriate...

...verification, such as DNA sequencing . In the example above, digestion with enzyme RE1 will linearize the 6200bp...that you get double confirmation. In the example above, digestion with either RE3 or RE4 will give a very...

...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of...

...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...window) New England Biolabs for more information about restriction enzyme buffers). If you select enzymes...

...approximately 50–60% confluent. For 293T cells this is about 2 × 10 6 cells in a 10 cm dish. Each 10 cm dish ...cell solution into the conditioned medium prepared above to make a new cell solution at a concentration of...

... working with the volatile reagents in the list above. Procedure Option #1 - Solution D Protocol Before...sure to prepare solution D (see reagent section above for the recipe). If using TRIzol®, jump down to ...

...the titer. Calculations In the experimental setup above, the following virus dilutions were used to transduce...v = Virus volume, mL *In the experimental setup above, the cells per well is 300,000 and the virus volume...

...and bathroom. These items should remain in the laboratory. When traveling between lab spaces, take your...

...the now gel purified DNA. Find more information about DNA quantification here . Tips and FAQ How do you...

...water and quantify the concentration (should be about 8ng/μl). Mix the annealed oligos with cut vector...

...suggested amount, instead of the other dry ingredients above. Loosely close the cap on the bottle (do NOT close...

... the right). When designing your plasmid, think about what DNA segments you will need to join to create...

...more at (Link opens in a new window) NEB's website about star activity . If you are digesting a large number...

... supernatant out of the tube if you are worried about losing the pellet. Dry with vacuum or by inverting...

...manufacturer’s instructions for specific instructions about extension time and temperatures. Initial Denaturation...

...cells in the untransduced well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor...

...Background In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same ...

...point. If your unknown sample’s absorbance falls above the range of the standard curve you will need to...

...dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer in a microcentrifuge...