We narrowed to 217 results for: Apple fluorescent protein

...integration of fluorescent tags in neurons. Their toolkit named ORANGE (Open Resource for the Application of Neuronal...engineered SECURE adenine base editor variants using a protein-truncation strategy with reduced RNA-recognition...abilities of C-to-T and A-to-G base editors into one protein. They fused an activation-induced cytidine deaminase...Editing) mediates targeted genomic integration of fluorescent tags in neurons.  Read the paper in bioRxiv ...with a com aptamer which binds an aptamer binding protein for packaging into a lentivirus-like particle. ...tagging Lentiviral capsid-based Cas9/gRNA ribonucleoprotein delivery Cytosine deaminase for RNA editing...bioRxiv Find the ORANGE toolkit   Cas9/gRNA ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle...

...amino acids) fragments for protein-protein interaction assays. Each target protein is fused to either LgBiT...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence...Luciferase Complementation assays can report on protein-protein interactions, drug-induced changes, and more... about BRET on our blog . Nano-Lanterns: Fluorescent proteins fused to Renilla luciferase ( Nano-lanterns...secreted version of the protein is available, making it ideal for a variety of applications. To learn more and...Both firefly and Renilla luciferase have short protein half-lives, making them useful as transcriptional...transcriptional reporters, but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively...

...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...

...mini counterpart label nearby proteins with biotin. The biotinylated proteins can then be isolated and identified...for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral preps...transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by ...primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from axonal boutons of neurons expressing iGluSnFR3...our bacterial plasmid set for expressing tagged proteins, and stay tuned for mammalian expression and AAV...therapeutic potential. Our partners at the Institute for Protein Innovation (IPI) recently released a set of highly-specific...antibodies against three of the six human glypican proteins (GPC1, GPC3, and GPC4). All IPI antibodies undergo...

...to change protein levels? Until recently, no such tool existed for rapid, customizable protein degradation...both short-and long-lived proteins, and it can even distinguish between protein variants when used with ...addition of exogenous TRIM21 protein along with an antibody targeting the protein of interest led to rapid...has deposited plasmids encoding fluorescent reporters and other proteins controlled by GAL4 and ZIF268....James and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell types, including...Plasmids Podcast! TRIM-Away: Targeted endogenous protein degradation Article contributed by Mary Gearing... cell populations, co-microinjection of TRIM21 protein and the target antibody works well. In larger cell...

...interest (or, if your protein is fused with a fluorescent protein), fluorescence microscopy can theoretically...Addgene.org Check Out Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors... place to screen your clones. Depending on the protein, we have used Western blots, dot blots, ELISAs,...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins are ...Thankfully the Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification of ...vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice. Current...optogenetics plasmids! Find AAV resources! Find fluorescent protein resources!    ...general rule of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then ...22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review of neuroscience...PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic tools derived from direct comparative...

...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types... to DNA. Others inhibit the recruitment of Cas proteins to the CRISPR-Cas complex. Recently, researchers... can include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed...used to purify a genomic locus and its associated proteins or RNAs through ChIP (enChIP) or biotin-streptavidin...

...Western blots are a great tool to identify a protein of interest in a complicated solution like cell ...— and what if you want to detect more than one protein in your sample? Or what if something weird happened...membrane, which is sturdier and retains the blotted protein better than a nitrocellulose membrane. If you have...probe for. Even using PVDF, some of your target protein will be removed from the membrane along with the...methods. If you are planning to detect multiple proteins through several rounds of stripping and reprobing...your signal by starting with the least-abundant protein and working toward the most abundant. If the abundance...to preserve your sample. Pro tip! Because some protein is lost each time you strip a blot, you should ...

...feature inaccurately. The best example is fluorescent proteins, which often come in closely related versions...misidentification, we augment the database with new fluorescent protein variants. The future of DNA feature identification...system was enhanced to allow searches for a perfect protein sequence match even when the DNA sequence has changed...Collections”, which are shareable databases of DNA and protein sequences, the next step is to enhance Collections.... We are working on a way for SnapGene users to apply features from a reference strain to newly sequenced...

...Assays monitoring impedance, fluorescence, mass/charge properties, protein thermal stabilization, and changes...to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection. Plasmid...Pooled Libraries Feedback Resources Solute carrier proteins (SLCs) are the largest family of transporters ...contribute to assess ligand action/engagement. Protein production is used for assays and to generate antibody... libraries target the solute carrier family of proteins. Library ID PI Description Human SLC Activation... CRISPR activation library targeting human SLC proteins. Human SLC Knockout Library 132552 Superti-Furga...Superti-Furga CRISPR knockout library targeting human SLC proteins. Mouse SLC Knockout Library 141436 Superti-Furga...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...specificity and applicability of base editing through protein engineering and protein delivery. Nature...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ..., or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...

...single-domain antibodies genetically fused to fluorescent proteins and used to visualize target antigens in...around using sdAbs in diagnostic applications. For example, fluorescently and radioactively labeled sdAbs...Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org  Plasmids encoding...to sdAbs becoming valuable tools for diverse applications. For example, RANbodies can be used as an alternative...inspired this versatile family of tools, the applications they have already enabled, and the discoveries...

...method for identifying protein-protein associations. This method uses a bait protein fused to a biotin ligase...PrM N and C   Detecting protein-protein interactions with DULIP Proteins rarely act alone. Molecular...which “bait” proteins are tagged with Protein A-Renilla luciferase and the “prey” proteins are tagged with...with Rosella, a pH-sensitive fluorescent biosensor A pH-sensitive fluorescent biosensor-based assay system...ligase to biotinylate proximate proteins in the cell. These candidate proteins can be enriched via biotin ...of fusion proteins, requires less biotin, and shows enhanced labeling of proximate proteins. Overall BioID2...BioID2 improves the efficiency of screening for protein–protein interactions. The Roux lab has deposited HA...

...will not produce the fluorescent protein while areas that are exposed will fluoresce. With these images,...transcriptional regulator Their system is based on the AraC protein that controls transcription from the PBAD promoter...reached the level of expression needed for most applications.   Figure 1: With blue light, the VVD...

...categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually...would only then kill the desired plants! PCR or fluorescent markers are currently used in these situations...could be achieved much easier than by PCR or a fluorescent microscope. First, red CRISPR-positive transformants...culture), which is great for a wide variety of applications. But some techniques require you to select against...

...Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] and Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86], target distinct...the SARS-CoV-2 nucelocapsid protein, one of the virus’s most abundant proteins and a critical component ...SARS-CoV-2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M]...transcriptase enzymes, inhibition of host cell proteins, and modifications to the pegRNA. The Britt Adamson... factors impacting prime editing and found the protein La (a small RNA-binding exonuclease protection ...prime editing with an endogenous small RNA-binding protein. Nature. 2024 Apr 3. doi: https://doi.org/10.1038...immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies #211756 (left panel) or #211757 (middle...

... art have a look at our Microbiology and Fluorescent Protein Resources and get started!  I learned a lot...paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling...cellular imaging with ultrasound. In his research he applies naturally occurring bacterial “molecular buoyancy...biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows dye visualization...

...macromolecules used in scientific research such as DNA or protein. It comes as no surprise then that stability of...crRNA (tracrRNA) which interacts with the Cas9 protein. These RNA molecules are vulnerable to attack by...gRNA is surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some...How to design your CRISPR gRNA CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...

...constitutively express a red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral cocktail...are easy to trace and not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color...A cocktail of three AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off ...morphology studies when used as part of a two part fluorescent tag-then-express system that allows for controlled...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments...