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We narrowed to 658 results for: EPO;

Showing: 121 - 140 of 658 results

  1. Twenty Years of Sharing Science!

    Type
    Blog Post
    ... When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and in 2022...ready-to-use antibodies. Now, we think of ourselves as a repository for DNA-based materials, including our popular... kits and libraries. This DNA-based materials repository contains 139,868 reagents from 5,821 labs in ... system for scientists using materials in our repository, from our customer and science support to our...

  2. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    ...Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope ...pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information Miguel Sena-Esteves...pcDNA-SADB19L Contains rabies viral RNA polymerase; see depositing paper for more information Edward Callaway ...tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab....

  3. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...at specified loci. Charles Gersbach’s lab has deposited mammalian expression constructs including pcDNA-dCas9...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9...Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted removal of H3K4me1...-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Here's what... intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren ...license. While the GENIE team tested and deposited versions with both PDGFR and NGR fusions for membrane...optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven enhancers...interneurons in mice. A) AAV expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... targeting astroglia. It has additionally been reported to have tropism for liver, vascular endothelial... muscle inflammation and degeneration has been reported (Muraine et al., 2020). AAV8 AAV8 has high transduction... the CNS (Han et al., 2023), and has also been reported to have tropism for liver and kidney cells (Issa...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration...cells utilizing various AAV serotypes. Scientific Reports, 15(1), 20927. https://doi.org/10.1038/s41598-025...

  6. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry...pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with ...

  7. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ... SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or...transcription factors with GFP Kevin White’s lab recently deposited a set of CRISPR plasmids to tag human transcription...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

  8. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ...Our plasmid repository succeeds because of our amazing community. The trust of our depositors to allow us...more scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside...manage our website, and continue to improve the repository. We love being able to provide a useful (and ...

  9. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ... to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-elite...publications. (As an interesting aside, Nature recently reported that the most cited work in history is a 1951 ... the best way to measure impact? As that Nature report also noted, papers detailing Nobel Prize-winning... for the impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649 ...

  10. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...fantastic research tool has been requested from the repository nearly 1,000 times in 2015! If you haven’t already... labs all around the world. We thank our many depositors for making these reagents available to the research...

  11. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology Plasmids with Addgene ...

  12. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble...now have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene...with scientists, but 2012 was marked by increased deposits (and requests) of biosensors, optogenetics, and...

  13. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...using the BE4max architecture. The new editors reported in this study have ~10-100-fold lower average ... gRNA multiplex. Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

  14. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes....ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be available...available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request service...ensuring we can maintain high standards for both our depositors and requestors.  Requesting Plasmids available...

  15. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction...glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor...

  16. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...sequencing results to perform quality control on deposited plasmids. We’ve also talked about how our new .... This saves us time as we are often analyzing deposits with many plasmids that contain similar features...match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...

  17. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...behaving mice to gather the following types of data: Report time-course and concentration of released DA in...optogenetically activated and inhibited neurons in live mice Report modulation of DA signaling during learning and ...moving mice to gather the following types of data: Report endogenous DA release from a single dopaminergic...looming stimulus in vivo in transgenic zebrafish Report DA release in live mice over the course of Pavlovian...

  18. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible...CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...

  19. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...tasked with was creating and sustaining a biweekly report of our social media analytics, something that had... meant I was responsible for establishing a new report and routine across the marketing and content team...a fantastic spreadsheet template to build out a report on. I was able to understand what metrics would...typical entry level tasks such as stuffing new depositor welcome kits, note taking for protocol video production...

  20. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges of creating...addition to the poster session was a first for our repository, and I was proud to be responsible for sharing...numbers of plasmid genome engineering tools – both deposited and distributed – have changed so dramatically...
Showing: 121 - 140 of 658 results