...at specified loci. Charles Gersbach’s lab has deposited mammalian expression constructs including pcDNA-dCas9...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9...Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted removal of H3K4me1...-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Here's what... intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren ... license. While the GENIE team tested and deposited versions with both PDGFR and NGR fusions for membrane...optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven enhancers...interneurons in mice. A) AAV expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer...

... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry...pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with ...

... SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or...transcription factors with GFP Kevin White’s lab recently deposited a set of CRISPR plasmids to tag human transcription...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...Our plasmid repository succeeds because of our amazing community. The trust of our depositors to allow us...more scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside...manage our website, and continue to improve the repository. We love being able to provide a useful (and ...

... to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-elite...publications. (As an interesting aside, Nature recently reported that the most cited work in history is a 1951 ... the best way to measure impact? As that Nature report also noted, papers detailing Nobel Prize-winning... for the impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649 ...

...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...fantastic research tool has been requested from the repository nearly 1,000 times in 2015! If you haven’t already... labs all around the world. We thank our many depositors for making these reagents available to the research...

...visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology Plasmids with Addgene ...

...after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble...now have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene...with scientists, but 2012 was marked by increased deposits (and requests) of biosensors, optogenetics, and...

...AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes....ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be available...available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request service...ensuring we can maintain high standards for both our depositors and requestors.  Requesting Plasmids available...

...using the BE4max architecture. The new editors reported in this study have ~10-100-fold lower average ... gRNA multiplex. Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...sequencing results to perform quality control on deposited plasmids. We’ve also talked about how our new .... This saves us time as we are often analyzing deposits with many plasmids that contain similar features...match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...

...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction...glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor...

...behaving mice to gather the following types of data: Report time-course and concentration of released DA in...optogenetically activated and inhibited neurons in live mice Report modulation of DA signaling during learning and ...moving mice to gather the following types of data: Report endogenous DA release from a single dopaminergic...looming stimulus in vivo in transgenic zebrafish Report DA release in live mice over the course of Pavlovian...

...tasked with was creating and sustaining a biweekly report of our social media analytics, something that had... meant I was responsible for establishing a new report and routine across the marketing and content team...a fantastic spreadsheet template to build out a report on. I was able to understand what metrics would...typical entry level tasks such as stuffing new depositor welcome kits, note taking for protocol video production...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible...CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...

...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges of creating...addition to the poster session was a first for our repository, and I was proud to be responsible for sharing...numbers of plasmid genome engineering tools – both deposited and distributed – have changed so dramatically...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids.... Anna Pyle’s lab at Yale University recently deposited a plasmid for expressing a novel reverse transcriptase...

...infrastructure, from our educational materials to our repository of open samples. We are, and have always been...the reach and impact of your work. Please email deposit@addgene.org if you would like to contribute to ...University of California Davis School of Medicine. This deposit includes frequently cited antibodies for use in...reagents, share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If you...

...challenging and requires specialized equipment. Reporters for studying gene expression have similar drawbacks...added to the palette. RUBY is a bright red pigment reporter whose expresion is visible to the naked eye in... enzymes and a spontaneous step. (B) RUBY is a reporter consisting of these three enzymes expressed under...References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and...