... traction.  This can carry a heavy price tag, especially if you're working on something other than software....  Your venture should continuously evolve and respond to feedback throughout its lifetime.  In this way...company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building external...discuss miLab.  Although many emails resulted in no response, I was still able to get through to a number of...

...Group of the NCI RAS Initiative, led by Dominic Esposito, has recently released a collection of 360 Ras...by restriction digest and Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists...tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which are available...TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2...altering the number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions...livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, most apicomplexan... kinases has been linked to several diseases, especially cancer. A great deal of chemical biology and ...

...immunogenic, meaning that they elicit an immune response from the body. Several factors contribute to the...a foreign antigen, triggering a humoral immune response. ADAs can inactivate the drug and increase drug...adverse immune reaction to the treatment. These responses vary depending on the person. However, in silico...to be presented by T cells to elicit an immune response. T cell epitopes are found in either the therapeutic...

...from mouse, and showed that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids ...expression across the majority of cellular pathways. Despite several years of research, the mechanisms of miRNA...connection between specific genes and an immune response has been a longstanding challenge. Using shRNA...with no deleterious effects on the in vivo immune response other than what would be the anticipated effect...

... immunogenicity, meaning they induce an immune response in the host. So why use a gamma-retrovirus in...meaning it does not induce the same high immune response as retroviruses. This virus can also transduce...population. Some adenoviruses are known to cause respiratory disease across species, as well as cancer in ...been engineered to induce a lower inflammatory response within hosts. Relative to the other viruses discussed...

...novel optogenetic variant of AcrIIA4.” CASANOVA responds to blue light quickly. Within minutes of inactivating...has high signal:noise ratio, high sensitivity, responds quickly, is photostable, and is compatible with...proteins, which change in protein conformation in response to light, offers a way to spatially and temporally...photoswitchable cPYP, which changes in conformation in response to blue light (445nm). The discovery and applications...

...science or even to discuss potential solutions. Despite all the talk, not much was being done to address...practices could be an actionable way to tackle widespread irreproducibility. Luckily, there were other ...meeting by the collaborators who could participate. Despite the group not having a public website, all the ...practices could be an actionable way to address the widespread issue of irreproducibility. Use your network...

...from Elizabeth Hubbard.   Haoyi Qiu: Pain response in mechanical allodynia Haoyi Qiu is a M.Sc. ...vivo firing frequency of PV neurons affect the response to light touch in mechanical allodynia. ...experimental approach. (B) CR-Cre mice demonstrate pain response due to blue light stimulation of dual virus-expressing...virus-expressing CR neurons. (C) Duration of painful responses in the left and right paw due to blue light activation...

...there’s no guarantee you’ll get a response on the first try (or any response at all). And while this method...free, you can always reach out to the PI or corresponding author of the paper it’s published in. That’... amount of money in reagents and consumables, especially for things like plates and gels, which either...

... of p53 tetramerization in response to DNA damage controls the p53 response. Gaglia G, Lahav G. Mol Syst...interact in cells and produce background signal (especially for some older split-GFP constructs or certain...functionality or binding of your target proteins, especially if the termini are important for their interaction...

...editing these cells to avoid a negative immune response (e.g., graft vs. host) as well as the introduction...allogenic therapies to prevent a graft vs. host response. Off-target effects are also important safety ... considerations for all CRISPR therapies, but especially concerning when using a systemic approach. Regarding... will not work in a meaningful way. Delivery, especially for systemic approaches, has its own unique set...

...held on Friday 16th and Saturday 17th September respectively. Join us to discuss advocacy efforts toward ...for students interested in leading the process despite the recent advance. Similarly, grad students throughout...currently compiling a database of institutional responses to the FLSA ruling. Similarly, from purely a salary...ways. Workshops will be run by experts in their respective fields: David Cameron, PhD - Director of Media...

...fetal microcephaly and Guillain-Barré syndrome. In response, the World Health Organization has declared a ... performing sensors and constructs encoding corresponding portions of the Zika virus genome with Addgene... American-lineages). Strain discrimination is especially important in the current outbreak because of ...the diagnostics into the hands of global health responders as soon as possible. Our longer-term aim is to...

...III), each responsible for transcribing different types of RNA: Polymerase I is responsible for ribosomal...ribosomal RNA, Polymerase II is responsible for mRNA and miRNAs, and Polymerase III transcribes tRNA and other...polymerase-associated proteins, CPSF and CstF, which are responsible for recruiting the cleavage and polyadenylation...

...time moaning about their troubles, Silent or unresponsive clams, who seem to ignore you and your questions...fellows are hesitant about expressing their ideas, especially if their supervisors are difficult people. The...will help you to communicate with confidence earn respect from your supervisor. Whenever possible, discuss... you even when you meet in person (silent or unresponsive clam), you will probably need support from your...

... inactivate specific genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in...-in systems, these systems remain valuable, especially in cases where CRISPR cannot be used (e.g. there... example shown in Figure 1, large sequences corresponding to exons 1 and 3 of the targeted gene have been...physiopathological events to which the chosen promoter responds. You can also use this method to tag an endogenous...

...product is toxic to cells at high concentrations. Despite their advantages, mammalian expressions systems...provides the highest level of protein expression. Despite their ease of use, it is important to note that... access and purification of a desired protein. Despite being a rather nascent technology, plant cells ...and copper inducible PHO5 and CUP1 promoters respectively. Yeast cells are grown in well-defined media...

...signaler. The pro of this system is the kinetic response time from signal input to readable output, which... is particularly powerful for in vivo imaging especially since JFs can cross the blood brain barrier and...molecule is added exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators... to direct your voltage sensor selectively is especially essential. If you want to learn more about how...

...daily tasks, meet cross-team project commitments, respond to any issues raised by team members, and plan ... all the things for which you are officially responsible on paper, and take some time to write down all... have to exercise in order to manage these responsibilities. If you are in charge of completing many ...management and organizational skills. If you are responsible for working with lots of students, managing inventories...

...SARS-CoV-2 toward destabilizing one’s natural immune response to Adenovirus (and/or to its partner, AAV) or ...transient due to the fact that AdV surface proteins (especially fiber’s knob domain, and hexon), as well as other...all recruit strong innate and adaptive immune responses that inactivate viral genomes or otherwise clear...Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector infected cells does not require...