We narrowed to 407 results for: HRE
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TypeBlog Post...systems must be transcribed into RNA, which occurs in three phases: initiation, elongation, and termination....RNA polymerase for transcription, eukaryotes have three RNA polymerases (Polymerases I, II, and III), each...
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Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...ability to differentiate into cells of any of the three germ layers: endoderm, ectoderm, and mesoderm. In... differentiate in vitro into derivatives of all three germ layers, form embryoid bodies, and form teratomas... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post... a living organism. This is the first post in a three-part series. We’ll first discuss how to get started... into embryos and then become larvae. There are three larval stages where larvae molt in order to continue... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...encapsulating the genome is majorly composed of three proteins that can be easily modified in order to...description of their experimental protocol. The three plasmids which have been deposited to Addgene are... -
Of Myc and Men
TypeBlog Post...antibodies (Evan, et al. 1985). Three bound to the mid-protein peptide, while three bound to the C-terminal peptide... -
PhD Applications After COVID
TypeBlog Post...await a reply. It’s a good idea to email around three professors per program. Most applications will recommend...application that you intend to work with, so reach out to three in case one isn’t able to respond to your email.... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog Post...needs to be used with spectral flow—you can run a three color panel or a 40 color panel! When preparing ...Similarity index across 12 different colors on a three laser spectral flow cytometer. Level of co-expression... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...able to immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to ...expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that inhibit the... -
CRISPR Antimicrobials
TypeBlog Post...antibiotic resistance Most antibiotics target one of three essential processes: cell wall synthesis, DNA replication... Phages could be used to deliver any of the three systems discussed above, with the caveat that phages... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...and that is what I would call bad science.” The three chapters in this section describe the challenges...case studies, and Zimmer provides several. In Part Three of the book, called simply “Old Science,” he describes... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...analyzed by comparing the threshold fluorescence of the viral DNA to the threshold fluorescence of a standard... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...later, Addgene is a company of fifty instead of three, and we are sending hundreds of plasmid requests...enjoyable events for employees. We now have a team of three (myself included) that plan big company events and... -
QC Sequencing Technologies at Addgene
TypeBlog Post...Shovill, apc, and NOVOPlasty. The pipeline contains three de novo assemblers, so if one fails to generate ... plasmid sequence, it moves to the next. If all three attempts fail, the QC team manually checks and assembles... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...Executive Director, Gary McDowell. On Dec 1st, the threshold at which salaried workers receive overtime payment...either all affected scientists are paid above this threshold or for tracking the hours that these scientists... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...you a million dollar startup package. There are three steps to chalk talk preparation: Define your research... Typically, a research vision is broken up into three aims. Two or four aims are also acceptable. Aims... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...analysis will open up a new window with three tabs. Each of the three tabs - Contributions, Indel Distribution... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...greater the risk of disease) there is no known safe threshold or “safe” exposure level. Unfortunately, the symptoms...important biomarker can be used to distinguish between three groups of people: Those who have mesothelioma ... -
Hot Plasmids Spring 2024
TypeBlog Post...the Voltron2 voltage indicator, created by the Schreiter Lab and the GENIE Project at Janelia, are now ...-to-noise ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity... -
Viral Production at Addgene
TypeBlog Post...titers obtained using ddPCR are generally two to three fold higher than those achieved using the standard...vector preparations are added to cells in culture. Three to five days later, cell cultures are inspected ... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...individually mutated three codons in eGFP to eliminate fluorescence. This created three inactivated eGFP ...