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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 185 results for: INV-E

Showing: 121 - 140 of 185 results

  1. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...PubMed Central PMCID: PMC3712628. 5. Dahlman, James E., et al. "Orthogonal gene knockout and activation ...and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...

  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...strain.  Most Addgene plasmids are maintained in the E. coli lab strain DH5a, but others may require strains...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...approach is to express and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...

  5. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...2003): 563-573. PubMed PMID:12490562. Karess, Roger E., et al. "The regulatory light chain of nonmuscle ...factors to repress the expression of genes primarily involved in development. These genes are known to play ... a comic book his daughter was reading. SHH is involved in the hedgehog signalling pathway, which is a...resemble spaghetti. We have two plasmids in our inventory from the lab of Eric Wieschaus that express sqh...Azplazu and Frasch, 1993). Tinman is specifically involved in the formation of the heart amongst other things...Transcriptional activation in Drosophila spermatogenesis involves the mutually dependent function of aly and a novel...

  6. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...–871. https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H., Lim, Z. F. S., & Ng, S. K. (2021... methods add an additional viral passage. This involves taking your first round of virus, called P1 (or...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    ...Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend to get stuck... at small and large scale.  This new technique involves selection in the cytosol (instead of on the yeast...

  10. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...PubMed Central PMCID: PMC6214761. Savell, Katherine E., et al. "A neuron-optimized CRISPR/dCas9 activation...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin. "Development and characterization of sindbis...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

  12. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...219. PubMed PMID: 27280971. 10. Epstein, Benjamin E., and David V. Schaffer. "Combining engineered nucleases...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway...

  13. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto...sample type. Sample Preparation This step can involve multiple smaller steps that all help to ensure ... Similar to Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve...

  15. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from ... plasmids encode a negative regulation system involving antisense RNAs or iterons that inhibits replication...

  17. Bacterial Expression Systems

    Type
    Collection
    ...Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli Strains for Protein Expression Protein... to purify it. Specific bacterial strains such as E. coli BL21(DE3) are used as biofactories to express...cytoplasmic and periplasmic protein expression in E. coli , Bacillus subtilis , and Pichia pastoris . ... (reconstructed) BiFC Dan Mulvihill 39866 pWA PAS-E BAD K-GAFm iRFP (reconstructed) BiFC Vladislav Verkhusha...reliably drive expression of a gene of interest in E. coli . The Marionette Sensor Collection (Addgene ...twelve genes using small-molecule inducers in the same E. coli strain. Addgene Blog Inducible Promoters Repressible...Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids...

  19. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Younger ST, Zhivich V, Najgebauer H, Allen F, Gonçalves E, Shepherd R, Doench JG, Yusa K, Vazquez F, Parts L...to perform functional screens to identify genes involved in a phenotype of interest. This blog post will... is a very low-efficiency process, and usually involves the need for single cell cloning and subsequent...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...PubMed Central PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors ...targeting each of the 1000 genes you’d like to investigate in your next CRISPR screen. Luckily, the Green... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...consequence, which genes are good candidates for being involved in the studied phenotype. For robustness of a ... generated knockout mice for (all of the genes involved as I recall). The lab then used these knockout...sequenced to identify which signaling molecules were involved in the phenotype. Surface proteins  My group works... These types of interactions to a large extent involve receptors expressed on the cell surface. Thus, ...
Showing: 121 - 140 of 185 results