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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 188 results for: INV-E

Showing: 121 - 140 of 188 results

  1. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... CBE Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko, J., Keene, A. et al. 2022. Course-based...ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new...

  2. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...PubMed Central PMCID: PMC3712628. 5. Dahlman, James E., et al. "Orthogonal gene knockout and activation ...and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...strain.  Most Addgene plasmids are maintained in the E. coli lab strain DH5a, but others may require strains...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...approach is to express and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...

  6. CRISPR Guide

    Type
    Collection
    ...36593413 Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang, H. H., & Sternberg, S. H... R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg, E. W., Goodale, ...edits. PASSIGE ( P rime- E diting- A ssisted S ite- S pecific I ntegrase G ene E diting), developed by the.... PMID: 26735016 Lee, J. K., Jeong, E., Lee, J., Jung, M., Shin, E., Kim, Y., Lee, K., Jung, I., Kim, ..., K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013). RNA-Guided Human...Siouve, E., Becavin, C., Depardieu, F., & Bikard, D. (2018). Genome-wide CRISPR-dCas9 screens in E. coli...e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S.,...

  7. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...2003): 563-573. PubMed PMID:12490562. Karess, Roger E., et al. "The regulatory light chain of nonmuscle ...factors to repress the expression of genes primarily involved in development. These genes are known to play ... a comic book his daughter was reading. SHH is involved in the hedgehog signalling pathway, which is a...resemble spaghetti. We have two plasmids in our inventory from the lab of Eric Wieschaus that express sqh...Azplazu and Frasch, 1993). Tinman is specifically involved in the formation of the heart amongst other things...Transcriptional activation in Drosophila spermatogenesis involves the mutually dependent function of aly and a novel...

  8. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...Fernandez-Perez, D., Sanchez, P. S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing stem cell heterogeneity dictates...titration. Can't wait? Browse our In-Stock AAV inventory or use our Packaged on Request service for thousands...

  9. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...–871. https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H., Lim, Z. F. S., & Ng, S. K. (2021... methods add an additional viral passage. This involves taking your first round of virus, called P1 (or...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    ...Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend to get stuck... at small and large scale.  This new technique involves selection in the cytosol (instead of on the yeast...

  13. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...PubMed Central PMCID: PMC6214761. Savell, Katherine E., et al. "A neuron-optimized CRISPR/dCas9 activation...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with...

  14. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin. "Development and characterization of sindbis...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

  15. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto...sample type. Sample Preparation This step can involve multiple smaller steps that all help to ensure ... Similar to Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve...

  16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...219. PubMed PMID: 27280971. 10. Epstein, Benjamin E., and David V. Schaffer. "Combining engineered nucleases...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway...

  18. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from ... plasmids encode a negative regulation system involving antisense RNAs or iterons that inhibits replication...
Showing: 121 - 140 of 188 results