We narrowed to 298 results for: IRES
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TypeBlog Post...look like (the 3rd sequencing trace). While it requires a little extra wet lab work, this is still a great...edits and look for off-target changes. This method requires a set of control cells to compare the sequencing...
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Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post..., BioID2 exhibits enhanced proximal labelling, requires less biotin, and allows scientists to more selectively...APEX2, although it can rapidly label proteins, requires H2O2 which can be toxic to living cells. To circumvent... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post... DNA outside of the recognition sequence. This requires that the vectors and DNA fragments contain these...sites recognized by the integrase. This method requires specialized vectors containing these integration... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...for how sensitive you can make a reaction. NLuc requires 100-fold less protein to produce the same amount...detecting traditional luciferases’ dim light emissions requires long acquisition times that may be impractical... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...difficult to isolate single registers or if it requires reading so many registers that Sanger sequencing...minimal cellular perturbation. Also, this method requires live, intact cells; the sequencing and qPCR methods... -
9 tips for a successful postdoctoral experience
TypeBlog Post...writing can take weeks to a couple of months and requires that all of the experiments have been completed...thoughtful engaging talk takes significant effort. It requires evaluation of all of the data and any recent relevant... -
Transferable Skills Guide: Teamwork
TypeBlog Post...world, so a process or project or mission that requires a broad knowledge base is going to necessarily...the type of team. Knowing what roles your team requires to function is certainly necessary, but equally... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...'t require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic) Digenome...doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to time since ... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...one since the formation of mEosFP’s chromophore requires a temperature below 30°C and this is not ideal...fluorescence localization data. However, this technique requires harsh fixation conditions to preserve cellular... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...spectrophotometry. Achieving the highest-quality DNA requires taking care at each step, but some purification... Using non-silica-based purification protocols requires less plastic and avoids the use of more hazardous... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...the R-loop structure. In order to bind DNA, Cas9 requires (1) a guide RNA, (2) a complementary DNA sequence...maybe because repairing a GBE-derived abasic site requires more cellular factors binding to DNA. However,... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...national interest waiver criteria however the EB-1A requires a higher standard of proving that you are one ...people engaged in researcher activities. The EB-1B requires that you provide two out of six forms of documentary... -
Using AAV for Neuronal Tracing
TypeBlog Post...neuronal tracing compounds and this is an area that requires further study. Traditional neuronal tracing methods...for neuronal tracing and network identification requires both antero- and retrograde labelling, synaptic... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...on or off the expression of shRNA. This system requires less work than using established methods and minimizes...thorough understanding of complex biological systems requires both a catalog of molecular players and contextualized... -
Bringing Renewable Energy to Addgene
TypeBlog Post...biosafety cabinet ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene... -
PEI Calculator for Planning AAV Packaging Transfections
TypeBlog Post...using this PEI Calculator. AAV packaging typically requires transfecting three plasmids at specific molar ... -
Celebrating One Million Plasmids Shared!
TypeBlog Post...would be an average of 673 plasmids each day. This requires tremendous coordination amongst the lab team, ... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog Post...biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular biological... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog Post...copy of your PO. If your purchasing department requires a quote in order to generate a PO, learn more ... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post...for DNA sequencing If your sequencing facility requires you to perform your own Big Dye PCR amplification...