We narrowed to 1,104 results for: Ide;

...between fluorescent proteins connected via flexible peptide linkers mCerulean, mVenus, and Amber (mVenus Y67C...mTurquoise2-mVenus(L68V) Goedhart et al. (2012) Structure-guided evolution of cyan fluorescent proteins towards ... S., Zhang, S., Lin, M. Z., & Chu, J. (2016). A Guide to Fluorescent Protein FRET Pairs . Sensors , 16...

... to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides an ... also have the unique potential to continuously divide while maintaining an undifferentiated state. Stem...

...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic...

...additional pages describing these reagents are provided below: Zinc Finger Genome Engineering Reagents...

...Carcinoid Tumors Bradley Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers in Intestinal...

...also like... Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein...

...transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo....

...together based on type or application. Browse our guides to find the plasmids and science information you...

... community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio...

...more attractive candidate for that ideal job. How can you identify your transferable skills? PLoS One ...collaboration and fail to identify it as a personal strength until it is identified by another person. Similar...when a new venture presents itself even outside of work, consider challenging yourself, take the opportunity...All while still paying bills and having a life outside my job. The same can be said about teamwork, communication...transferable skills so important? It is important to identify which transferable skills you possess because ... and making connections, avoid falling into an identity trap where you assume your technical skills define...wants to hear a specific time that you thought outside the box to solve a problem. When they ask about...

...high fidelity ligase—and when is an alternative ligase a better choice? And what is ligase fidelity anyway...are "higher fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase...annealed complementary regions. Why use a high fidelity ligase? Numerous applications have been developed...developed to take advantage of the high fidelity of Taq and other thermostable DNA ligases. These applications...applications detect specific nucleotide sequences with high specificity and quantitative accuracy and even include... include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase Detection...PCR amplified fragment) and treated with a high fidelity thermostable DNA ligase. If the target sequence...

...Other Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416...myc HIS SMN1 His, Myc CMV Spinal muscular atrophy Gideon Dreyfuss 71875 pET-Sac-Abeta(M1-42) APP T7 Alzheimer's...pFBD-BirA_LRRK2:2141-2527 LRRK2 His, Biotin acceptor peptide tag polH Parkinson's Cheryl Arrowsmith 210986 pHTN-HaloTag_C9orf72... found to match all requested criteria. Please consider using fewer filtering options. Browse all Neurodegeneration...

... contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene...within the Addgene catalog. These IDs allow you to unmistakably identify a plasmid available through Addgene...VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier format for lenti-Cas9-VQR is...will be assigned an Addgene ID #, RRID, and resolvable compact identifier. Addgene works hard to ensure...including plasmids. All plasmids at Addgene have unique ID numbers associated with them, which serve as unique...about each plasmid item. Given a specific Addgene ID # listed in a publication, you can quickly pull up...the “Articles Citing this Plasmid” section. Having IDs linked to these metadata and publications help to...

...is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how to use...incredible new CRISPR technologies developed and widely adopted in the past few years, as well as refreshing...refreshing the existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime... PRIME editing Please check out the updated guide for a great introduction to what CRISPR technologies...

... viral attachment and fusion. Once inside a host cell, new evidence in PLoS Pathogens shows that the Ebola...week within two months. That said, Ebola is not considered a “terribly infectious disease” – infection occurs...via body fluids not through the air and that’s considered unlikely to change – but the illness has been...thought we’d do a little digging and assemble a rough guide to the science of Ebola and related resources. Ebola...

....    If this video is helpful, you can find our complete collection of protocol videos on the Addgene...antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies...

...then, in having a guide that curates this information. And so I created the Codon Guide to Synthetic Biology...The video channels are also underrated and I recommend checking them out, especially since videos on synthetic...knowledge but little biology experience.) The Codon Guide to Synthetic Biology One of the best ways to learn...begin their journey into synthetic biology. The guide is a curated list of research papers, blogs, free...send me suggestions. The most useful parts of the Guide are probably the papers and textbooks. I’ve selected...to find. I hope that many people will find this guide useful as they start their synthetic biology journey...

... and adenoviruses in this easy-to-use guide. Print out the guide and use it for quick reference when you're...information on viral vectors? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service...pages. Click on the Image Above to Download the Guide ...

...alone. So, if you consider yourself part of the scientific community, get used to the idea of working on ...our kit process substantially and have plenty of ideas for further improvements. Types of teams There ...the first few meetings and we’ve volunteered our ideas, skills, and time to the cause ever since. Unlike... necessarily need a diverse group of people to provide that base. Sometimes there’s just too much work...ever taken has declared me an introvert, and I identify as one.  But some time around 10th grade, I snapped... a happier person. What does a good team need? Besides that all-important sense of purpose, there are ...multiple people: someone to coordinate, someone to decide, someone to delegate, etc. If you find that there...

...three or four guides directed towards that gene as there can be a large difference in guide potency. Thus...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to a ...scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there has...required for Cas9-based activation. CRISPRa for genome-wide studies One of the best uses for Cas9 activators...  Which Cas9 activator should I use? There are a wide variety of activators you can use for your experiments...transcription. That being said, we generally pick guides by putting the promoter of the gene into a gRNA...(2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome Biol 16:...