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Showing: 121 - 140 of 148 results

  2. Lentivirus Plasmids

    Type
    Collection
    ...17532 pLTR-G 2nd Envelope Expression of VSV-G glycoprotein (Indiana strain). Use with packaging construct...

  3. COVID-19 Resources

    Type
    Collection
    ...proteases.( 3 ) BSG - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily, binds to ...

  4. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TCTAATTGGTCTGTACCTaaagtggccgctaaggAAGCCCCCTGGGCTGATA lycat TAL3482 & TAL3483 TCCTTCATCTTCATCCAGcggcgctgggaggacgACCGCACACACATGAGCA...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Hirokawa, K.E., Bohn, P., Joines, K.M., Peng, H., Hawrylycz, M.J., Phillips, J.W., Hohmann, J.G., Wohnoutka...

  7. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...smegmatis Rock NA Varies 176,286 Neelamegham Human GlycoGene CRISPR Library 140961 Knockout Human Neelamegham...

  8. Genetic Code Expansion

    Type
    Collection
    ...) 7-HCou Aminoacyl (7-hydroxy-4-coumarin-yl) ethylglycine Bacterial TAG Kenneth Johnson 198323 pRSF-G1TMSNKRS...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... in the CIDAR MoClo Parts Kit are available as glycerol stocks in a single plate with every plasmid or...

  10. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...how to generate a monoclonal cell line from a polyclonal pool of stable cells....how to generate a monoclonal cell line from a polyclonal pool of stable cells. Transducing cells with ...with lentivirus results in a heterogenous polyclonal population that varies in the number of integration ... as the lower expressing clones take over the polyclonal cell pool. Generating a monoclonal cell line ... Hemocytometer or other cell counter Reagents Polyclonal stable cell pool DMEM high glucose, Corning 10...FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Polyclonal stable cell pool: see our protocol for generating...cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is always undergoing selection, it’...

  11. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...cells, resulting in a more homogenous (but still polyclonal) cell population. Depending on the transducibility...regularly to confirm transgene expression. As polyclonal populations of resistant cells start proliferating...etc. Pro-Tip This selection method results in a polyclonal cell population, meaning that the transgene has...from different cells in the population. Once the polyclonal populations are growing well and have been sufficiently...expression. Over time, transgene expression in a polyclonal population may drop. This is because cells that...consider generating monoclonal lines from the early polyclonal populations. Sample Data Figure 1: Generation...

  13. Protocol - How to Streak a Plate

    Type
    Protocol
    ...from Bacterial Culture Introduction If you have a glycerol stock or stab culture of bacteria and need to ...burner. Obtain the approrpriate bacterial stab or glycerol stock . Using a sterile loop, pipette tip or toothpick...punctured area of the stab culture or the top of the glycerol stock. Pro-Tip If you use a wire loop you can ...

  14. Kit Free RNA Extraction

    Type
    Protocol
    ...Lithium Chloride (for precipitation step, Option B) Glycogen (Optional) Caution Make sure to read the SDS (...anticipate your RNA yield to be small, RNase-free Glycogen may be used as a carrier to facilitate RNA precipitation...

  15. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Bacteria on an LB Agar Plate Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids Recovering... the bacteria, you can proceed with Creating a Glycerol Stock . You can now isolate your plasmid DNA from...

  17. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Benzonase/DNAse I (Millipore 71205-3) 40% Polyethylene Glycol 8000 (PEG) + 0.5 M NaCl Cell lysis buffer (50 mM... and thaw a new working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution: Dissolve 400 g of PEG 8000...

  20. CRISPR Library Amplification

    Type
    Protocol
    ...freeze-thaw if not suspended in cryoprotectant like Glycerol or DMSO solutions. Quantify the individual Maxipreps...
Showing: 121 - 140 of 148 results