We narrowed to 178 results for: Lta;

1. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   ...Any IVA primers can be used in combination to simultaneously perform modifications, simply by adding all...

2. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   ...Kamuzinzi, R., Ledent, V., Blaudez, D., Sanders, D., Revuelta, J.L., Boles, E., André, D., and Frommer, W.B....

3. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

   Type

   Blog Post

   ...and dissemination of experimental protocols, simultaneously allowing scientists to actively comment, annotate...

4. Overcoming the AAV Size Limitation for CRISPR Delivery

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   Blog Post

   ...Constructs containing the ADAR2 truncation ADAR2DD(delta984-1090) are approximately 4.1 kb in length, allowing...

5. New Tools Enable CRISPRa for Neuroscience Applications

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   Blog Post

   ...PMCID: PMC6412672. Zhou, Haibo, et al. "In vivo simultaneous transcriptional activation of multiple genes...

6. Hot Plasmids: Fall 2024

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   Blog Post

   ...target multiple genes in M. abscessus either simultaneously or stepwise, achieving mutation efficiencies...

7. Twenty Years of Sharing: Addgene's Viral Vector Service

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   Blog Post

   ...Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral vector...

8. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   ...analysis, which compares hundreds of samples simultaneously.  The current version of ICE software can analyze...

9. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   ...from the cell. Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates...

10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    ...amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single...

11. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    ...and easily obtainable growing conditions. As a facultative aerobe, it can thrive with or without oxygen....

12. Five Popular Model Organisms

    Type

    Blog Post

    ...often as every three weeks so scientists can simultaneously observe several generations of mice at a time...

13. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    ...of artificial guide RNAs that can be used to simultaneously target cellular RNA to specific intracellular...

14. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    ...pathway. Accomplishing this usually involves simultaneously introducing a homologous DNA repair template...

15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ...Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect...

16. CRISPR 101: Validating Your Genome Edit

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    Blog Post

    ...main advantage of NGS is that it allows you to simultaneously validate edits and look for off-target changes...

17. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    ...Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid ...

18. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    ...Damien Wilpitz is a laboratory research management consultant and coach in Boston, MA. He specializes in assisting...

19. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    ...gene is deleted, it is normally noted with a Greek delta (Δ).  Additional resources We've provided an overview...

20. Genomic Deletions in Mammalian Cell Lines

    Type

    Collection

    ...plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not...complement oligo ( e.g., sgRNA-A in Table 4 ). The resultant oligos would be 25-mer oligos. However, if the...the reverse complement oligo. In this case, the resultant oligos would be 24-mer oligos ( Table 4 ). Design...