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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 526 results for: MEA;

Showing: 121 - 140 of 526 results

  1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...Understanding Cas9 biophysics enhances HDR We first measured Cas9 dissociation rates and discovered that the... of approximately 6 hours. To our surprise, we measured identical dissociation rates for catalytically...replacement rates of approximately 1%. This is by no means a large number, but it was attained without any ...

  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...Sanjana et al., 2014). It is a dual-vector system, meaning it is typically used in conjunction with a second... be carried over to the gRNA lentiviruses. This means that if you received lentiCas9-blast lentivirus ...(Gi)-mCherry from this virus is cre-dependent, meaning that once injected, the DREADD will only be expressed...

  3. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...modified Tango beta-arrestin recruitment assay to measure receptor activation for more than 300 GPCRs. In...peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek et al., 2017...learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic studies ...

  4. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...biorender.com     The modularity of antibodies means that it is relatively easy to physically divide ... vivo.     Of course, the lack of an Fc portion means that most of the common anti-IgG secondary antibodies...monovalent Fab’ or Fab fragments. Higher avidity means that F(ab’)2 fragments may have overall stronger...

  5. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... which often forces scientists to use invasive measures to perform in vivo optogenetic experiments. Intracranial... electrophysiology, a technique that’s used to measure electric current of ion channels in living cells...electrophysiology. All thirty were functional and their measured photocurrent amplitude, wavelength sensitivity...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...imaging applications, smaller affinity reagents means higher resolution. Many of the below are also small...in an ~50kD protein. Minibodies are bispecific, meaning that each scFv can target a different antigen; ...That is the question… or is it? This post isn’t meant to convert anyone to downsize their affinity reagents...

  7. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...result in unwanted changes at non-target sites. Means to decrease these so-called “off-target effects”...specificity of Cas9 derived from S. aureus. This means you can likely apply similar rational mutations ..., Chen et al. 2017 used single molecule FRET measurements to determine which portions of Cas9 might be...

  8. CRISPR in the Clinic

    Type
    Blog Post
    ...researchers had cured 24 mice of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to...success rate in clearing misfolded protein levels as measured in the blood. More information is expected soon...difference, then the treatment will not work in a meaningful way. Delivery, especially for systemic approaches...

  9. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... highly influenced by this paper. Influence is measured by the number of citations and the context of ...You can also create intersects in your feed which means only papers than contain all of your search terms...by papers published each day, week, or month. A measure of the number of times a paper is shared online...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...initiation site and open circles mark pixels used to measure signal along the dendrite. D) Fluorescence signal...signal as a function of the distance between measured pixel and the soma. The initiation site identified...Figure 4). Figure 4: ONE-GO biosensors measure the formation of Gα-GTP by BRET. The biosensor ...

  11. Of Myc and Men

    Type
    Blog Post
    ...specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, substance...sequence context.    So, do Schüchner, et al.’s results mean the end of 9E10’s time in the saga of the Myc tag...the variety of formats you can find it in likely mean it will remain an important tool for the foreseeable...

  13. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... as real time PCR is a PCR technique used for measuring a starting DNA concentration using PCR. qPCR requires...fluorometer feature built into the thermocycler to measure that fluorescent output. With this fluorescent ...droplet is analyzed by a droplet reader, which measures the fluorescence amplitude of each droplet. The...

  14. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...the expression level in a specific system (by measuring the fluorescence intensity); and 2) to visualize...activated by a specific excitation wavelength. This means that the emission wavelength can change. In a few...studies (24-30°C). Brightness: Brightness is a measure of how bright is the emission. Brightness is calculated...

  15. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...intensity and transduction efficiency of neurons were measured. On average, AAV.PHP.eB and AAV.PHP.S had 1.5-...1x1011 vg per mouse and the level of transduction, measured via GFP expression, was determined 3 weeks later...1x1012 vg per mouse and the level of transduction, measured via GFP expression, was determined 3 weeks later...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...level of resolution that they can achieve. This means that regardless of how powerful of an objective ...you image samples before and after expansion and measure the degree of agreement between them to ensure ...fragmented appearance post-expansion, but that doesn’t mean we should start re-conceptualizing actin filaments...

  17. Tips for arabidopsis transformation

    Type
    Blog Post
    ...Healthy plants produce lots of seeds. More seeds mean more chances to recover a transformant. Use young... expose the flowers to Agrobacterium. More seeds mean more chances to recover a transformant. Use lots...-10 plants per construct I transform. More seeds mean more chances to recover a transformant. Remove mature...

  18. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...as "the best way" to communicate their science, meaning that they will heavily evangelize for the mode ...and contextualizing data. Often overlooked as a means of scicomm. Digital or print distribution.  Typically...for conceptualization not possible through other means. Easy to combine multiple types of media. High barrier...

  19. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...viral constructs is a useful tool as it provides a means to quickly check that a construct is functional ... 2nd- and 3rd-generation vectors? What does this mean for transfer vectors? A4: The lentiviral packaging...Addgene transfer plasmids are replication-deficient meaning they can be used to create virus that is capable...

  20. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...et al. originally developed the ELISA assay to measure antibody levels, scientists have since adapted ...typically use a competitive or inhibition ELISA when measuring small molecules that cannot bind efficiently to...the amount of antigen in the sample: more antigen means less antibody available to bind to the reference...
Showing: 121 - 140 of 526 results