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We narrowed to 154 results for: N-pac

Showing: 121 - 140 of 154 results

  1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...genomes. Cas9s from other species, like S. aureus and N. meningitidis require different PAM sites than SpCas9... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...

  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...Central PMCID: PMC4267879. 10. Samulski RJ, Muzyczka N., 2014. AAV-Mediated Gene Therapy for Research and...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...

  4. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman N. (2012) Introduction to Fluorescence Microscopy. iBiology...architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.” It is based...antibody performs differently and some antibodies impact the performance of other antibodies. But, once ...the right protocol, multiplex IF can give you impactful and beautiful results. Performing an immunofluorescence...fixation maintains sample morphology with minimal impact to the target epitope. There are multiple methods...exclusive to IF, but they can have a significant impact on the outcome of your IF experiment. Many of the...choose for your experiment will have a significant impact on the protocol you use and the ultimate outcome...

  5. p53 Pathway

    Type
    Collection
    ...SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper B, Brayton C, Park...weight (53 kDa) when run on an SDS-PAGE gel. Cancer Impact p53 is the most frequently mutated gene (>50%) ...disorder known as Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized as gain-of-function...

  6. TALEN Guide

    Type
    Collection
    ...149-53. PMID: 21248753 . TALEs for the masses. Rusk N. Nat Methods 2011 Mar;8(3):197. Pubmed PMID: 21473016...FokI nuclease operates as a dimer, cutting in the spacer region between two distinct ZF target sites. (B...

  7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...2017).Walton, R. T., Christie, K. A., Whittaker, M. N. & Kleinstiver, B. P. Unconstrained genome targeting... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our SpCas9 containing...Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific modifications... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can...

  8. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...(2009): 495-505. PubMed PMID: 19300443. 7. Holic, N., Seye, A.K., Majdoul, S., Martin, S., Merten, O.W...lentiviral infection begins with your cells, both your packaging cells and your target cells. Poor tissue culture...harvest after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy...benefitting your cells, medium exchanges may also impact the quality of the lentiviral particles you harvest...density, volume, incubation time, and MOI can all impact the success of your lentiviral infection. To ensure...

  9. Bacterial Expression Systems

    Type
    Collection
    ... (vitamin B12) Escherichia coli Remy Loris 47663 pAC-EsaR-I70V Plac 3OC6HSL (Quorum sensing molecule) ...BiFC Lynne Regan 168257 168472 pMRBad-C-wtBlc pET11a-N-DiB2 DiB2 (reconstructed) BiFC Jens Meiler Additional... direct recombinant proteins to the periplasmic space between the inner and outer membranes. Targeting... 113634 pSCrhaB2 PrhaB Rhamnose Burkholderia cenocepacia Miguel Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL...

  10. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Dennys-Rivers C, Huffenberger A, Braun L, Rinaldi F, Wein N, Meyer KC, Solano S, Nguyen K , Lang E, Kaspar AA,...trans-splicing, and protein replacement to reverse the impacts of this disease.  As a non-degenerative, monogenic...

  12. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...likelihood of an off-target artifact. Increasing the N in your experiment is the only way to effectively ...in mind, but likely will not have a significant impact on your data. You are trying to generate a knockout...depending on your platform, off-target effects should impact your experimental design differently. Yes, it is...optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few...

  13. Lentivirus Plasmids

    Type
    Collection
    ...David Rawlings and Andrew Scharenberg 22502 pCEP4-tat N/A Expression of tat . Increases viral titer. Sergey...shRNA of interest flanked by LTRs Packaging plasmid — contains packaging and regulatory genes; one plasmid...Addgene lentiviral packaging, envelope, and transfer plasmids Viral...Lentiviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset... third-generation Envelope plasmid — contains packaging gene env This collection highlights popular second...second- and third-generation lentiviral packaging, envelope, and transfer plasmids available at Addgene.... Feng Zhang Return to top Popular Lentiviral Packaging Plasmids ID Plasmid Generation Description PI ...

  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... https://doi.org/10.1242/dmm.002733 Idalia, V.-M. N., & Bernardo, F. (2017). Escherichia coli as a Model...more model organisms that have left a historical impact on the scientific community. (And, check out this...

  15. Immunology Research Plasmids and Resources

    Type
    Collection
    ...ATF2, HFB1-GDNF GH1 growth hormone 1 GH, GH-N, GHN, hGH-N GH2 growth hormone 2 GH-V, GHL, GHV, hGH-V GHR... TRUSS UBR1 ubiquitin protein ligase E3 component n-recognin 1 JBS, MGC142065, MGC142067 UBXN1 UBX domain..., C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1 IFITM1 interferon induced transmembrane...neuroblastoma RAS viral (v-ras) oncogene homolog ALPS4, N-ras, NRAS1 PIK3CA phosphoinositide-3-kinase, catalytic...CD331, CEK, FGFBR, FLG, FLJ99988, FLT2, HBGFR, KAL2, N-SAM, OGD FGFR2 fibroblast growth factor receptor 2...NT4, NT5, NTF5 NTS neurotensin NMN-125, NN, NT, NT/N, NTS1 NUDT6 nudix (nucleoside diphosphate linked moiety...SDC2 syndecan 2 HSPG, HSPG1, SYND2 SDC3 syndecan 3 N-syndecan, SDCN, SYND3 SDC4 syndecan 4 MGC22217, SYND4...

  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...avoid target sites that code for amino acids near the N’ terminus of the protein, in order to mitigate the...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9.../doi.org/10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J,...

  17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...10.1038/onc.2014.470 Taghbalout A, Du M, Jillette N, et al (2019) Enhanced CRISPR-based DNA demethylation...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression or silencing...particularly large protein, and this fusion allows for packaging into viral vectors and targeted delivery into ...

  18. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...biocomputing and biological circuits.     References 1. N. Roquet et al., Synthetic recombinase-based state ...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple... et al. "Permanent genetic memory with> 1-byte capacity." Nature methods 11.12 (2014): 1261-1266. PubMed...

  19. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... proteins: The recombinase is split into inactive N- and C-terminal fragments and placed under the control...ATAACTTCGTATAgcatacatTATACGAAGTTAT 13 bp inverted repeats + 8 bp spacer (34 bp) lox2272, lox5171, lox511, loxN, lox66/lox71...TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site ...GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC 13 bp inverted repeats + 8 bp spacer (48 bp or 34 bp) F3, F5, FRT14, FRT15 minimal FRT...feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene blog...feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene blog...

  20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...Williams P, Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant AAV Viral Vectors Pseudotyped with...recombination between homologous pairs before viral packaging even begins. This spontaneous recombination persists...2020). In general, we observe that 0.1 - 0.8% of packaged viral genomes contain a recombined/functional ...which can lead to off-target expression of the packaged transgene in neurons and glia.   Check the recombination...v14715, titer 2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively low recombination...
Showing: 121 - 140 of 154 results