We narrowed to 416 results for: Numb

1. With Our New Viral Service, We're Taking CRISPR Further!

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   ... Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids...

2. Synthetic Photobiology: Optogenetics for E. coli

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   ...processes. Chemical effectors are suboptimal for a number of reasons, including their potential toxicity ...factors. These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded chromosomally...

3. Viral Vectors 101: Viral Applications

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   ...tools in your experiments? Let’s unpack the myriad number of emerging viral applications and give you some...high infection frequency and the viral titer (the number of viral particles capable of infecting a host ...

4. Sharing is Caring: But How to Distribute Open Hardware?

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   ...state-of-the-art devices for research. There are a number of new platforms (such as openUC2, which I am a...framework FIJI are just three of the constantly growing number of "community-driven" software that make everyday...

5. 7 Tips to Secure a STEAM Internship This Summer

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   ...make the ultimate case: why you? 7. Strength in numbers Encourage your close peers to come along for the...should use these guidelines consistently across a number of years as you apply for internships, scholarships...

6. Building and Validating Recombinant Antibodies: A Case Study

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   ...increase the number of functional constructs that were created. This greatly limited the number of clones...

7. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ...Many fluorescent proteins are available and the number of fluorescent proteins is steadily increasing....normalized to the value of EGFP). The higher the number, the higher the theoretical brightness. The theoretical...

8. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   ...developed assays to test large numbers of cancer variants across a large number of genes as systematically...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ...but poor template digestion can result in a high number of background colonies carrying the unmodified ...REPLACR-mutagenesis’ median efficiency is 75%. If these numbers hold up across labs, REPLACR-mutagenesis could ...

10. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    ...Musunuru: We have been using the kit to target a large number of genes involved in cardiovascular and metabolic... is that you could potentially sequence a large number of individuals and find several mutations in the...

11. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    ...much of this decrease is due to an increase in the number of universities’ whose plans are unknown (up to...postdocs, and indeed we have been contacted by a number of postdocs who are now planning unionization at...

12. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ...several decades: “Fix the Numbers of Women” focuses on increasing the numbers of women participating in...

13. Plasmids 101: Golden Gate Cloning

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    ...Although efficiency may decrease with an increased number of fragments, or the ligation of very small/very... problems can be overcome by screening a higher number of potential clones. Golden Gate assembly has a...

14. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...part of your routine. Set a goal to read a certain number of papers each week to keep up with the research...respect to my organization strategy. There are a number of software options for helping you manage your...

15. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...orthogonality. You can create an almost limitless number of DNA binding proteins with Cas9 guided by different...and programmable that you can make a very large number of regulators. That basically means larger genetic...

16. Working with Nuclear Receptors

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    ... feature a simplified user interface, expanded numbers of curated molecules, including 400 or so coregulators...been possible without the development of a vast number expression and reporter plasmids and the free exchange...

17. Engaging with science and society at pgEd

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    ...molecular biology and epigenetics. So, I sought out a number of different opportunities to broaden my training... technology studies (STS). I also worked with a number of organizations in public engagement efforts, ...

18. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ...hydrochloride, as a potential treatment for a large number of rare genetic diseases. Spontaneous mutations...cause rare diseases. They now plan to test a large number of other disease-linked Arg-mutant proteins both...

19. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...lifespan, or infertility and can arise due to copy number, expression level, and site of integration of the...hemizygous or heterozygous background to reduce copy number or expression level effects.  Another challenge...

20. Hot Plasmids - October 2020

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    ...is particularly challenging as they are great in number, generally very small, and can contain different... showed reduced proliferation and had a greater number of red cells as measured by flow cytometry. Cells...