We narrowed to 257 results for: PORA

1. In Living Color: The Skinny on In Vivo Imaging Tools

   Type

   Blog Post

   ...proteins, organelles, and living cells in a spatiotemporal manner, which is not possible with conventional...

2. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

   Type

   Blog Post

   ...was developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (...

3. Fostering communication in remote collaborations

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   Blog Post

   ...sure there is a point person responsible for incorporating everyone’s suggestions into a final version ...

4. An Addgene Summer Internship

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   Blog Post

   ...to differentiate which clone had successfully incorporated Syntaxin3 into its backbone. Once we had gotten...

5. Hot Plasmids - October 2022

   Type

   Blog Post

   ...string of 10 amino acids, EQKLISEEDL, that can be incorporated into your protein of interest and recognized...

6. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   Blog Post

   ...changes to the genome, thus providing an avenue for temporary/reversible CRISPR modification. In addition to...

7. Addgene at the ASM Microbe 2016 Conference

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   Blog Post

   ...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...

8. Choosing Your Perfect Empty Backbone

   Type

   Blog Post

   ...have to make sure that your plasmid has been incorporated into the host organism, usually achieved with...

9. History of CRISPR Cas - A tale of survival and evolution

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   Blog Post

   ...phages to test if the exogenous DNA would be incorporated into the bacterial genome. Cas genes, which ...

10. CRISPR Challenges: Standardization and Homology Directed Repair

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    Blog Post

    ...strand annealing (SDSA) and single-strand DNA incorporation (ssDI). "Easi-CRISPR for creating knock-in and...

11. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post

    ...acids. Unfortunately, the location of biotin incorporation and to a lesser extent, the number of added ...

12. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post

    ...new tools and protocols that they can easily incorporate into their own labs. This week we are pleased...

13. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    Blog Post

    ...sequencing and analysis approach can be easily incorporated into standard QC regimens and will provide scientists...

14. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post

    ...cushy”—tenured faculty position for the grind of corporate life”.   In fact, there are a lot of good reasons...

15. Viral Vectors 101: Transductions

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    Blog Post

    ...nature. All three of these methods involve DNA incorporation into the cell, but the mechanisms vary. Why ...

16. Summer SciComm Series: Master of SciComm

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    Blog Post

    ...environment. Over time, this interest came to incorporate other facets which occupied my attention, until...

17. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    ...transcription.However, this system lacks spatio-temporal regulation, and is difficult to reverse, making...

18. 10 Great Guest Posts We're Thankful For

    Type

    Blog Post

    ...potentially be used to report upon and/or engineer “temporally ordered biochemical events” such as those that...

19. New and Upcoming Viral Vectors - December 2019

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    Blog Post

    ...Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise in vivo measurements...

20. Extracurricular Activities for a Strong Science Career Path

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    Blog Post

    ...highlight the importance of taking a break and incorporating EAs into your routine.  Getting started: Planning...