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We narrowed to 651 results for: PPL

Showing: 121 - 140 of 651 results

  1. Finding Your Perfect Job After University

    Type
    Blog Post
    ...received many applications, employers were requesting that only scientists with an MSc or higher apply. It was...scientific research would be very competitive, and so I applied for a summer role as a Research Scientist at the... edge of London to gain relevant experience. I applied for this summer position as I felt I needed more...I had to reassess what I wanted to do and so I applied for a role in Industry. I wanted to get "a real...university to study a Masters of Science I decided to apply to study a Master of Science (MSc) in Human Molecular...virology classes in California and enjoyed them, so I applied for a role at a commercial virology testing company...enjoy helping people and decided to compromise and applied anyway. I later found out the job was with Addgene...

  2. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...re used in a number of research and clinical applications. Most of the time, when someone speaks about...deeper penetration into a tissue, useful for applications like IHC, or be able to access an epitope folded...that would size-exclude an antibody. In imaging applications, smaller affinity reagents means higher resolution...antibody’s ‘Y’. They are often used in clinical applications. Fab fragments can be created in lab in situations...Shahied, 2004). They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins...antibodies are still an excellent option for many applications, and frankly, if ain't broke, don't fix it!  ...flexible in your approach to affinity reagent applications. Whatever affinity reagents you use, one thing...

  3. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...single-stranded oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild...proceeds from the maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template...consider that already-tested CRISPR alternatives are applicable in many situations. In the case of hypertrophic... need to be much, much lower before clinical applications could be considered. It is important to consider...consider the question of broader applicability of CRISPR germline editing. Nature News mentions that the...only way to answer questions of efficiency and applicability. Given moral and ethical concerns surrounding...experiments are needed before we can consider clinical applications for germline editing. Although this paper represents...

  4. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...your media with supplements such as magnesium salts, buffering agents, and/or supplying additional carbon...are, you’ll even have DNA left over for other applications, like PCR, cloning, transfection, or long-term...wash step or use a different strain. For most applications, Addgene uses and recommends DH5𝝰! This strain...your E. coli to ensure it is suitable for your application. We have previously reviewed many common lab ...culture media Changing to a richer media or adding supplements can improve your cell density, leading to an ...normally recommended antibiotic concentration. Add supplements to the media You can achieve higher cell density...

  5. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...EthoVision. Consult the literature to find all the applications of optogenetics relevant to your field. Ultimately...behavior, certain technical considerations still apply. Testing stimulation First, In lieu of electrophysiology...steps and technical considerations are directly applicable to the rat [6]. Technical differences include...models. Beyond rodents, optogenetics has even been applied to other species such as non-human primates [7,...PMC3595120. 4.Mattis, Joanna, et al. "Principles for applying optogenetic tools derived from direct comparative...rat lines: tools, techniques, and optogenetic application to dopamine-mediated reinforcement." Neuron 72.5...sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex...

  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...is an application in which antibodies that have been joined to fluorescent molecules are applied to a ... Primary Antibody This is the first antibody applied to your sample, which will bind to your target ...Unsurprisingly, this is the second antibody you apply, which binds to the first antibody and is conjugated...distribute antibodies validate them in a variety of applications, including IF (or IHC or ICC), and will share...Resources on the Addgene blog Learn about research applications of antibodies in Introduction to Antibodies ... for Me? Read about another common antibody application in The Basics of Western Blotting Resources ...

  7. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...researchers could apply this technique to efficiently deliver large repair templates for applications that require...with a homologous repair template. It is this application - the creation of sequence-specific knockins ... allele of a single gene at once. Therefore, applications that require simultaneous modification of all...clones, making it unsuitable for most in vivo applications.  Third, even in the most optimized tissue-culture...components as ribonucleoproteins (RNPs) that can be supplied separately from the viral vector. This delivery...you’ve been able to check out some of the many applications of AAV in some of our other blog posts and have...

  8. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...reproducible if its findings can be predictably applied to future work. Importantly, no one should expect...studies, as reported by drug companies attempting to apply the findings from these studies, are quite low (...low rates of reproducibility. Failed attempts to apply irreproducible results to future work cost both ...solutions based on what I’ve learned. I hope you can apply some of these solutions to your own research and...ridiculous to expect that one should be able to apply someone else's new and incredibly useful technique...information about fluorescent proteins so you can best apply these technologies to your own research needs. Other...evidence shows otherwise. Others will go on to try to apply these findings in their own work ultimately wasting...

  9. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ... chain reaction (LCR)-overview and applications." PCR Methods Appl 3.4 (1994): S51-64. PubMed PMID: 8173509...or DNA base pair mismatches (8-11). For some applications, there cannot be any end-joining activity, and...regions. Why use a high fidelity ligase? Numerous applications have been developed to take advantage of the...Taq and other thermostable DNA ligases. These applications detect specific nucleotide sequences with high...Ligase are not replacements for T4 DNA Ligase in applications such as routine cloning or DNA library preparation...ligase chain reaction in a PCR world." PCR Methods Appl 1.1 (1991): 5-16. PubMed PMID: 1842922. 13. Wiedmann...

  10. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...the technology in different labs for different applications.” Li’s team noted that CRISPR/Cas9 has been ...disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult stem cells...repair,” Doudna said. “And it can't be too hard to apply. These papers suggest it might work well enough ...with size limitations. In addition, non-viral applications are also possible through the use of nanoparticles....  “CRISPR Genome Editing Technology and its Application in Genetic Diseases: A Review.” Current pharmaceutical...4. Uddin et al. 2020.  “CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future...

  11. Science Career Options

    Type
    Blog Post
    ...critically and carry out in-depth research is applicable to a wide range of industries and is a highly...skills acquired from a science degree that can be applied to many roles. These types of skills show that ...sectors such as forensics currently have too many applicants compared to the number of available roles). Make... Make your resume stand out Before you begin to apply for a job, it is useful to search online job websites...your research, it’s time to start sending out applications. Check out our other career posts for more specifics...careers and to find advice on crafting the perfect application. Additional Resources on the Addgene Blog Learn...

  12. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent proteins everyday...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...Light-inducible Proteins (FLiPs) Fluorescent proteins can be applied in a long list of creative ways. In her graduate...utility of these FLiPs in a variety of research applications and particularly notes their ability to be precisely...efficient product of HMOs that could be used to supplement newborn diets and promote the formation of a ...

  13. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...cell type doesn’t always lend itself to direct application of virus. However, the cargo capacity of AAV ...gamma-retro viruses. This is large enough for many applications, but in some cases multiple AAVs (co-infection...and AAV. If you’re wondering more about what applications these viruses are good for, we have plenty of...Adenovirus-Mediated Gene Delivery: Potential Applications for Gene and Cell-Based Therapies in the New...Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713. DOI: 10.3390... Campos MD, Materatski P. An Overview of the Application of Viruses to Biotechnology. Viruses. 2021 Oct...

  14. Hot Plasmids - February 2022

    Type
    Blog Post
    ...editing applications.  Figure 4: Overview of CasMINI for genome engineering applications. Image ...stability in cells, and use it in a variety of applications. This set of new anti-FLAG frankebodies includes...to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling various targets... and anti-HA frankenbodies have a variety of applications, including live-cell imaging, single-molecule...challenges for in vivo delivery and cell engineering applications. CasMINI, a new miniature CRISPR-Cas system ...

  15. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...protein for your application. Selecting the best fluorescent protein for a specific application can seem like...under conditions that best mimic the intended application. The three key properties for live cell imaging...experimental set up. The practical brightness takes all application specific parameters into account, including the... picture of what can be expected in a 'real' application than the theoretical brightness. Consequently...brightness in a system closely mimicking the future application. In figure 2 we provide an example of the comparison...

  16. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the luciferase transfers...multicolored nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion... a luciferase substrate like coelenterazine is supplied in the culture medium to allow RLuc8 to produce...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite the...furimazine. In addition to the previously described applications, the enhanced signal of eNL allows their use...characterizing Nano-lanterns and eNL for many different applications. For long-term imaging, especially where photobleaching...

  17. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...emission wavelengths, which are pivotal in their application in biotechnology (Grimm & Lavis, 2022). These... substrates/peptides and antibodies. Example applications of this include coupling to an antibody, whereby...typically bind to. Stability of fluorescence: For applications that involve ranges of pH, and significant lengths...light, is another factor to consider. If your application requires longer periods of exposure, you’ll want...descriptions, or technical manuals. Specific applications and matching dye/ligand choices For effective...Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to have compatible excitation...more stable fluorescence for super-resolution applications. Other current advancements include work exploring...

  18. MXS Chaining

    Type
    Blog Post
    ...develop diverse fluorescent reporters is a useful application of high-throughput cloning. MXS Chaining is one...plasmids for fluorescence imaging or flow cytometry applications in mammalian cells. The modules used in this...transgene expression, or a variety of other clever applications. In one example, modular constructs generated...methods are suited for particular downstream applications. The tradeoffs between various cloning methods...technique to engineer various constructs with applications in flow cytometry approaches in mammalian cell...quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental needs. Let us ...

  19. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...many CRISPR applications, and they’ve also had success in clinical gene therapy applications. Read on to...to learn more about the current (and future) applications of lentiviral vectors! Lentiviral vector history...barcoding libraries have been used for complex applications like lineage tracing in heterogeneous tumors...developed integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a...Integrase-defective lentiviral vectors: progress and applications. Banasik MB, McCray PB Jr. Gene Ther. 2010 Feb...

  20. Hot Plasmids - November 2023

    Type
    Blog Post
    ...Figure 1: Choosing the best PE6 variant for your application. Image reused form Doman and Pandey et al., 2023... CreVLPs, and eVLPs for in vivo and in vitro applications.   Figure 2: Modular features of DIRECTED...approach for experimental therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D...interactions or map a local proteome, but some applications have been challenging or inaccessible. The Ting...externally-timed bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record...Lab’s use of Anti-PSD-95 [K28/43R] under the Application section of the Addgene antibody page, and learn...
Showing: 121 - 140 of 651 results