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We narrowed to 205 results for: PREP

Showing: 121 - 140 of 205 results
  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...! Now, let’s head west, intrepid reader! Protein Prep The first step in any western blot is to lyse your...minutes) at 37 °C, if you suspect aggregation in your prep. If your proteins are the wrong size on your gel...
  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...
  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...product (see figure). Template A high purity plasmid prep significantly increases the success rate of site...
  4. Colony Formation Titering Assay

    Type
    Protocol
    ...polyethersulfone filter, Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge...
  5. DNA Quantification

    Type
    Protocol
    ...NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click ...
  6. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...you can find a protocol for kit-free plasmid mini-prep at the bottom of this page. Last Update: Feb. 8,...culture of bacteria . Pro-Tip Refer to appropriate DNA prep protocol for volume of bacteria to grow (low copy...
  7. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...appropriate imaging system. Figure 1 Recombinant antibody preps should have 2 clear bands at ~50 kDa and ~25 kDa... Pro-Tip Addgene only uses recombinant antibody preps that have a purity of ≥90% but some labs use unpurified...
  8. AAV Packaged on Request

    Type
    Collection
    ...service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification...protection levels. We offer 1,091 in-stock viral vector preps, delivering high quality and ready availability ... many researchers, scientists sometimes require preps beyond our current in-stock capabilities. To meet...start experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce,...All-in-One Pricing The price for our Packaged on Request preps is all-inclusive of several important steps: MTA... varies by requesting organization Viral vector preps remain subject to the terms and conditions of the...priority. During this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes...
  9. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...highlight some of the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids...
  10. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...some of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles...
Showing: 121 - 140 of 205 results