Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 364 results for: Pol

Showing: 121 - 140 of 364 results

  1. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses... inactivated by the complement system in serum (DePolo et al., 2000). Other envelope proteins for pseudotyping...–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL...

  3. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...with a “sloppy” version of PCR that results in a polymerase error rate of up to 2% per nucleotide position...modifications: 1) increased concentration of Taq polymerase, 2) increased PCR extension time, 3) increased...degenerate primers and the high-fidelity Phusion polymerase are used to introduce point mutations into the...

  4. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...of useful, entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking...almost anyone with enough practice. I always take a poll of scientists in training to see how many in my ...other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular question...

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs from deleting...

  8. Synthetic Biology - Overview

    Type
    Collection
    ...Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...cation channel, activation of which results in depolarization and subsequent firing of an action potential...results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential generation...spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through hydrogen...

  10. Distribution to Industry

    Type
    Collection
    ...be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is available ...

  12. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...proteins whose fusion improved the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding ...rXRCC1, or the DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion...

  13. Control AAV Preps

    Type
    Collection
    ...Deisseroth 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive 9, PHP.eB Feng 27056...

  14. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...with BioRender.com. Top7 gets its name from “topology of protein” diagrams, which show the interactions...seen so far!). They started by designing a novel topology, then iteratively generated 3D models and amino...

  15. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...walking tour of Buddy the Elf’s journey from the North Pole to New York City. Check out their video below. ...interpretation of Buddy the Elf's journey from the North Pole to New York. #ELF #DeckTheLab #ProudPIhttps://t....

  16. TALEN Plasmids and Kits

    Type
    Collection
    ...specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. View...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs...this starting point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase... time Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein...

  18. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters...message delivered by many of the speakers.  As Dr. Polka said as she started things off, “We are all capable...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...Expression of your gene of interest (MCS with a β-globin poly A) & EGFP from a bidirectional tet-responsive promoter...
Showing: 121 - 140 of 364 results