...250 kDa Similar in size 8% 20–250 kDa Similar in size 10% 10–100 kDa Similar in size ...conjugated to primary antibodies). Direct westerns are simpler and faster than indirect ones and can be used in...visualize proteins on a PVDF membrane without staining; simply wet your membrane with 20% methanol and place it...proprietary systems are fast (often <10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches...

...generation simpler, as described in an earlier post on generating CRISPR mouse models. For simple knockout.... It’s also made targeted insertions relatively simple in C. elegans and bacteria. But CRISPRing typical...microinjection, the efficiency of Easi-CRISPR for simple knock-ins and floxed models varies from 8.5-100%...Easi-CRISPR have also recently developed i-GONAD as a simple method for delivery of genome editing components...

...molecules in molecular biology labs (for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages...following is a description of a technique published by Simpson, Sacher, and Szymanski in January 2016 in Viruses...to remove unbound cells. To detect bound cells, simply lay each membrane on an agar plate selective for...27536293. PubMed Central PMCID: PMC4971087. 3. Simpson, David J., Jessica C. Sacher, and Christine M. ...Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David J., Jessica C. Sacher, and Christine M. ...

... being inserted into new target sites. This is similar to how retroviruses, like HIV, replicate. Class...can be inserted between the transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a...vertebrates and integrates in human cells at rates similar to retroviral vectors. The hyperactive version ...piggyBac has an integration efficiency in human cells similar to retroviral vectors. The hyperactive PB transposase...with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also prefers to integrate at ...

...SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke, James Blish, and John W. Campbell...strung together, it can read many base pairs simultaneously to increase specificity. This modular approach...follow a DNA-binding and DNA-cleaving strategy similar to the ZFNs, but instead of recognizing nucleotide...base editor (ABE) enzyme in 2017. ABEs rely on a similar strategy as CBE but use a ssDNA adenosine deaminase...research focuses on the use of molecular dynamics simulations and machine learning to develop a predictive ...

... With this technique, multiple genes can be simultaneously silenced if a consensus sequence is used (~...micro RNAs (amiRNAs) are frequently used. Although similar, amiRNAs are engineered from endogenous precursors...respectively. Short hairpin RNA (shRNA) is conceptually similar to the microRNA precursor mentioned above and is... tissue for downstream characterization. To simultaneously address both limitations of the traditional...Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen. Plant Physiol 178:989–1001...

...your antibody can distinguish between two very similar proteins. Additionally, they require variable expression...reduced or eliminated antibody signal. If you see a similar antibody signal between your control and your knock-out...protein expression experimentally increased. The simplest version would be to use cells that do not express...you more confidence when both antibodies exhibit similar results. Unfortunately, it can be difficult to ...antibodies to your protein of interest give you very similar results, while the third looks entirely different...

...and replaced it with a double-bonded oxygen. This simple enzymatic reaction enabled conversion between the...Researchers in the Xueli Zhang lab were making similar observations around the same time. (The papers ... journal on the same day.) This team created a similar construct that they called a Glycosylase Base Editor...(Huang et al., 2024). BE4max, a CBE, generated similar deletions at a lower frequency. Their data suggest...editing is still advancing rapidly. If it follows a similar path to base transition editing, we can expect ...

...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS)...viral genome sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...routinely producing AAV. We believe that this simplified sequencing and analysis approach can be easily..., Leenay R, May AP, Paulk NK (2019) Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of ...

...overstated, we can also learn a lot from Nature’s simpler solutions to transmitting forces over long distances...engineer it to suit our purposes? In search of simple biological machines R bodies can repeatedly ...These questions can be addressed using R bodies: simple, force-generating protein machines that can extend...

...the goal of encouraging user contributions (using simple web forms) while also maintaining a relatively ...register with a valid email address).  One may also simply attach important references that serve to further...more powerful ways, and the advanced search page simplifies the process of building advanced multi-parametric... few years with an emission peak around 600nm?  Simply input these parameters and you’ll find matching...

...follow multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires...if you know that your protein of interest has a similar spectrum. For example, here's a screenshot from...Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / ...of fluorescent proteins, imaging four colors simultaneously should be pretty straightforward. Thank You...

... next cloning experiment early on. Beyond these simple but powerful display improvements, when you click...for copy and paste functionality. Sequence Similar to the plasmid maps, the linear sequences hosted...hosted here are now interactive and have similar hover over features. As with the map tab, clicking on two...Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN...

...students focus on the application process, and simplify access to academic resources, we created STEAMid...worried about any eligibility provision, don’t guess. Simply reach out to the program coordinator with your ...through. The vast majority of programs tend to ask similar questions, thus devoting some time to writing a...Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources among economically...

... thus contain complex internal cell structures similar to those of plants and animals. Unlike bacteria...mechanism of homologous recombination to facilitate simple gene replacement/mutation. Yeast expression plasmids...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast... Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “high ...

...generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...factors driven by one promoter. While lentivirus has similar rates of reprogramming when compared to MMLV-derived...advantages of this method are that it’s relatively simple to implement and doesn’t require time-consuming...advantage of using RNA to reprogram cells is that it’s simple and efficient. It has a high efficiency of reprogramming...also take less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact...

...described above can be used for similar applications and have similar advantages, though with some caveats...the lack of an Fc region. The lack of Fc also simplifies analyses when used in structural studies and ...Alternatively, Fab fragments can be used as a block in similar scenarios. By applying unconjugated Fab fragments...

...RNA. To protect the RNA from degradation, several simple methods have been employed which modify the sugar...make your gRNA more efficient on a budget is to simply swap out several of the ribonucleotides for deoxynucleotides...resulting in less off-target events. While both have similar advantages, bridged nucleic acids with N-methyl...RNA to control the entire editing system with a simple LED light. Lighting up your gRNA with fluorescent...

...freezer.   RNA extraction methods evolved into a simple protocol still used today There are many alternative...for RNA extraction and purification. There is one simple method that works, and variations to that method...effectively separated from DNA and proteins by a simple extraction protocol with guanidinium thiocyanate-phenol-chloroform...View our protocol for RNA extraction! Making the simple protocol even more foolproof (still without a kit...

...organization scheme for multi-part DNA assembly. Simply put, it assembles individual DNA components into...begin with a promoter and end with a terminator. A simple TU could be comprised of just three parts: promoter...assembled into TUs is key to understanding MoClo. By a similar principle, TUs can be further complexed into multi-TU...Genetic circuits Most things in biology are not so simple, and engineering complex cellular behaviors requires...