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Showing: 121 - 140 of 618 results

  1. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR FPs) ... emiRFPs in your applications.  There is a whole set of spectrally distinct (e)miRFPs available. The numbers...Methods 16, 171-174 (2019). Matlashov, M.E. et al. A set of monomeric near-infrared fluorescent proteins for...

  2. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...communication to another person.  In an interview setting, this means persuading an employer that your past... to ask and answer these questions for a diverse set of audiences.  Every scientist needs to be able to...skills in science communication will be a positive asset throughout your career? 1. Make scicomm practice...of this as a jumpstart or a refresher for your skillset rather than a permanent fix. Treat every interaction...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...get you started, here are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins...catalytically dead version of the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier...

  5. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    .... An important difference between the original setting and the new one, previously unaccounted for, may...may lead to different results. Different setting, different population, new genetic variation, or different...results of all four of those tests and not just the subset of those that return a particular result. Anything...

  6. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...now available in lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA...the Addgene Advisory Board) says: "When you're setting up to do a genetic screen, you need to focus on...

  7. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...instructions use NEB buffers. Nonetheless, if you are setting up a double digest with NEB enzymes, the app is...of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence... can’t guarantee every reagent in your chemical closet is in this app. However, if you get quizzed randomly...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash...obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...the MIT Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on ...model systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used...now available at Addgene, so on your marks...get set...RUSH. Boncompain et al., Nat Methods 2012. Mar...; the Cell-free expression test kit represents a set of 88 clones in a pCellFree vector that enables protein...

  10. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...around the scientific world, so you might wonder what sets HiBiT apart. While the classic Green Fluorescent...and cellular autofluorescence (Tsien, 1998). What sets HiBiT apart from these tags is its use of bioluminescence...explore TP53 biology. They first identified that in a set of patients with gastric cancer (GC), TP53 was one...early-onset GC and one with a mutation at R273H to match a mutation frequently found in late-onset GC. ...Site-Linked Chemosensitivity in Early- and Late-Onset Gastric Cancer. Cancer Medicine, 14(8). https://...

  11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ... based on the identity and order of a particular set of inputs, may be used to better understand and engineer...on the state cannot be undone and also limit the set of possible downstream states that can be achieved... register to record all permuted substrings of a set of chemicals. Specific examples of arrangements that...possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate gene expression) that uniquely...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ...Hochedlinger MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...

  13. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...simply pause and take a deep breath before the panic sets in. Most of the time, that’s enough–but if it’s ... lab meetings, you’ve developed a valuable skill set that will be useful in nearly any other job you may...

  14. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... many of the same elements as a STAR answer: a setting, a conflict, and a resolution. Stories usually ...interviewers will not have the same experiences as you, so set them up for success by giving them sufficient background...

  15. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting that researchers...being able to recognize it as a part of your skill set later on. If you are new to research and the lab,... define this skillset. After sifting through dozens of career advice articles, I settled on defining problem...qualified to be an astronaut. But, knowing that this skillset is a part of your toolkit is important. Paired...

  16. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...or circuits with functional relevance following a set hierarchy. MoClo originally developed from Golden...unpredictable cases, MoClo provides two answers: first, sets of parts with characterized behaviors are available...

  17. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...Whenever an order is placed on the Addgene website, it sets off a chain reaction of events throughout all of...dealing with the samples to newer lab members. We can set ourselves up for success by giving explicit instructions...one of these steps can fail but, in our current setup, there are no explicit instructions on what to do...but critical logic. Some steps fail “hard”, they reset the whole procedure. Other steps fail “soft” they... and capital cost of getting up to speed with a setup is almost always worth it. Because labels are applied...

  18. Pipetting Protocol

    Type
    Protocol
    ...Without moving past the maximum possible setting on the pipette, set the desired volume of the liquid on the...tip is properly set on the pipette to ensure pipetting accuracy. Once the pipette is set and you have the... pipette tip, the volume display which shows the set volume for the the pipette, and the tip ejector ring...

  19. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...paper from Ed Callaway’s lab (Fischer, KB. 2019) set-out to systematically test the potential causes of...expression in vivo.  Since DIO/FLEx vectors contain two sets of homologous recombination sites, they hypothesized...should now be able to make smaller adjustments to a set of injections into your animal model. For example...of AAV vector we produce containing a DIO/FLEx cassette using next-generation sequencing (NGS) (Guerin...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...CRISPR knockout screens have been used in several settings, and are fantastic tools for unbiased discovery...library before using them to understand how they are set up. Using the “User Upload” alternative, you furthermore...convenient for you to later identify how you were setting up the experiment.  Output_NotFoundList: A text...approach is to use smaller libraries targeting subsets of genes. With such an approach, you lose the unbiased...differentially expressed genes found in a gene expression dataset are causal for a phenotype of interest. Selected...
Showing: 121 - 140 of 618 results