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... all of biology) require large data sets. Whether these data sets include videos of behavior, many many...

...get you started, here are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins...catalytically dead version of the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier...

.... An important difference between the original setting and the new one, previously unaccounted for, may...may lead to different results. Different setting, different population, new genetic variation, or different...results of all four of those tests and not just the subset of those that return a particular result. Anything...

...now available in lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA...the Addgene Advisory Board) says: "When you're setting up to do a genetic screen, you need to focus on...

...instructions use NEB buffers. Nonetheless, if you are setting up a double digest with NEB enzymes, the app is...of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence... can’t guarantee every reagent in your chemical closet is in this app. However, if you get quizzed randomly...

...Hochedlinger MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...

...DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes. Samwer M, Schneider MWG, ...

...fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash...obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...the MIT Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on ...model systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used...now available at Addgene, so on your marks...get set...RUSH. Boncompain et al., Nat Methods 2012. Mar...; the Cell-free expression test kit represents a set of 88 clones in a pCellFree vector that enables protein...

...around the scientific world, so you might wonder what sets HiBiT apart. While the classic Green Fluorescent...and cellular autofluorescence (Tsien, 1998). What sets HiBiT apart from these tags is its use of bioluminescence...explore TP53 biology. They first identified that in a set of patients with gastric cancer (GC), TP53 was one...early-onset GC and one with a mutation at R273H to match a mutation frequently found in late-onset GC. ...Site-Linked Chemosensitivity in Early- and Late-Onset Gastric Cancer. Cancer Medicine, 14(8). https://...

... based on the identity and order of a particular set of inputs, may be used to better understand and engineer...on the state cannot be undone and also limit the set of possible downstream states that can be achieved... register to record all permuted substrings of a set of chemicals. Specific examples of arrangements that...possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate gene expression) that uniquely...

... many of the same elements as a STAR answer: a setting, a conflict, and a resolution. Stories usually ...interviewers will not have the same experiences as you, so set them up for success by giving them sufficient background...

...simply pause and take a deep breath before the panic sets in. Most of the time, that’s enough–but if it’s ... lab meetings, you’ve developed a valuable skill set that will be useful in nearly any other job you may...

...variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting that researchers...being able to recognize it as a part of your skill set later on. If you are new to research and the lab,... define this skillset. After sifting through dozens of career advice articles, I settled on defining problem...qualified to be an astronaut. But, knowing that this skillset is a part of your toolkit is important. Paired...

...or circuits with functional relevance following a set hierarchy. MoClo originally developed from Golden...unpredictable cases, MoClo provides two answers: first, sets of parts with characterized behaviors are available...

...Whenever an order is placed on the Addgene website, it sets off a chain reaction of events throughout all of...dealing with the samples to newer lab members. We can set ourselves up for success by giving explicit instructions...one of these steps can fail but, in our current setup, there are no explicit instructions on what to do...but critical logic. Some steps fail “hard”, they reset the whole procedure. Other steps fail “soft” they... and capital cost of getting up to speed with a setup is almost always worth it. Because labels are applied...

...Without moving past the maximum possible setting on the pipette, set the desired volume of the liquid on the...tip is properly set on the pipette to ensure pipetting accuracy. Once the pipette is set and you have the... pipette tip, the volume display which shows the set volume for the the pipette, and the tip ejector ring...

...CRISPR knockout screens have been used in several settings, and are fantastic tools for unbiased discovery...library before using them to understand how they are set up. Using the “User Upload” alternative, you furthermore...convenient for you to later identify how you were setting up the experiment.  Output_NotFoundList: A text...approach is to use smaller libraries targeting subsets of genes. With such an approach, you lose the unbiased...differentially expressed genes found in a gene expression dataset are causal for a phenotype of interest. Selected...

...paper from Ed Callaway’s lab (Fischer, KB. 2019) set-out to systematically test the potential causes of...expression in vivo.  Since DIO/FLEx vectors contain two sets of homologous recombination sites, they hypothesized...should now be able to make smaller adjustments to a set of injections into your animal model. For example...of AAV vector we produce containing a DIO/FLEx cassette using next-generation sequencing (NGS) (Guerin...

...may generate irreproducible data in a research setting, but these events could have disastrous health ...criteria are not exhaustive. Mismatch tolerance settings are often limited to off-target sites of <4 bp...clinical approval. It is key to maintain a consistent set of standards as the field seeks to generate reproducible...streptavidin and next-generation sequencing (BLESS) (Crosetto et al. 2013). GUIDE-seq and BLESS detect double-stranded...25503383. PubMed Central PMCID: PMC4320661. 14. Crosetto, Nicola, et al. "Nucleotide-resolution DNA double-strand...

...summer role was incredibly valuable because it was a set duration of only three months and made a good job... in a developing tropical country. This is why I set up a placement working in a rural health clinic in... can be a glut of individuals with similar skill sets. This is not to say that all commercial companies...