... of the Max Planck Society in Tübingen, Germany. She studies vertebratedevelopment using optogenetic approaches...sequence assembly of the zebrafish genome was published in 2013, revealing that about 70% of human genes...for generating transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust...zygote transforms into an organism with a clearly established body plan including head, eyes, and tail. Compared...different methods have been developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality...zebrafish early embryonic development by scanned light sheet microscopy." science 322.5904 (2008): 1065-1069....

... whom I worked, for feedback on my presentation, she said “it was such a valuable extension to our curriculum...our CRISPR blog posts Download our CRISPR cheat sheet Read our science communication blog posts Resources...

...Lee is a graduate student at Stanford University. She studies cell fate maintenance in the stomatal lineage...

...response. Then, just when Andrew was about to suggest she deprioritize the project, Maria’s screens of AsLOV2...

...National Emerging Infectious Disease Laboratories.  She is interested in genome replication and transcription... the viral mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional...

...proteins for subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University ...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5...

...addition to helping with daily laboratory operations, she also works on improving laboratory efficiency through...samples can be stored in? Different shelves? Different racks on each shelf? Give each container a specific...samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received as DNA...large collections. Many companies, such as Thermo Fisher and Micronic, provide 2D barcoded tubes. 1D barcodes...done easily with a barcode reader and an Excel spreadsheet. Start by scanning the sample barcodes into the...and use this ID to name the corresponding Excel sheet.3. Be specific  - When assigning a physical location...

...Annette Sievers, Ph.D., for her tips and guidance. She is way better at protein purification than he is....

...that control who a female wants to mate with and if she decides to raise their offspring or leave them to... encouraged to not just wait for papers to be published, but to engage in ongoing, real-time dissemination...A paper on stickleback fish science was first published in 1867. It said they were hard to raise because...

...only a princess who suffered a terrible fate when she pricked her finger on the spindle of a spinning wheel...Groucho Marx, so the gene was aptly named groucho (Fisher and Caudy, 1998). Interested in plasmids that contain...

...external video recorder and a mic clipped to her shirt. She rolled several long takes, and we cut it up in post...hopefully they’ll be just as interesting as the finished product for you, the viewer. While you’re here...

... Excellence in Molecular Plant Sciences in 2019. She joined Sichuan Normal University and became a lecturer...Originally published Apr 13, 2021 and last updated Aug 12, 2021. This post was contributed by guest bloggers...University, Jieze Zhang, an intern in the Yang Lab, and Sheng Yang, a professor in synthetic biology from the ... optimizing strains with low efficiency.       Sheng Yang obtained his Ph.D. from Shanghai Institute ...biomedical applications.   Yiwen Zhang entered the Sheng Yang Lab of the CAS Center for Excellence in Molecular...

...with a PhD in reproductive biology and genetics. She loves to communicate the latest and greatest in scientific...companies supply gRNA sequences, such as GenScript, ThermoFisher Scientific and OriGene, or you can order them...

...systems in the Sashital Lab, Iowa State University. She is actively interested in science communication and...in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK. Two-step-SHERLOCK...sequences, Cas12a activity gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves...2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​s​ensitivity ​E​nzymatic ​R​eporter...sensitivity in the zeptomolar range while One-step-SHERLOCK has a sensitivity in the femto-aptomolar range...light illuminator. Approach: Highly similar to the SHERLOCK diagnostic test, this test varies in the sequence...JG, Gootenberg JS, Abudayyeh OO, Zhang F (2019) SHERLOCK: nucleic acid detection with CRISPR nucleases....

...Systems Biology department at Harvard University. She is a scientist with a passion for photosynthetic ...

... writer at the Institute for Protein Innovation. She writes about all things IPI, from the scientists ...all bind outside the ligand-binding pocket, their sheer size impeding ligand attachment. The five other ...recommended for use in flow cytometry. Based on the established applications of the original hybridoma monoclonals...

...Morgridge Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of...

... enhancer element Gordon Fishell 83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAVrg pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent...hDlx enhancer element Gordon Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...

... TAG Wenshe Liu 127415 pEVOL-AcKRS-CloDF PylRS M. mazei acyl-lysine derivatives Bacterial Wenshe Liu 127445...Bacterial Wenshe Liu 137908 PylRS-AS PylRS M. mazei fluorophenylalanine derivatives Bacterial TAG Wenshe Liu...consider. If you are working off of a previously established protocol, make sure to match the growth medium... pEVOL-AckRS PylRS M. mazei AzHeK Bacterial TAG Wenshe Liu 140009 pAS_4xMma PylT_FLAG-Mma PylRS PylRS ...pEVOL-pylT-AznLRS AznLRS M. mazei azidonorleucine Bacterial TAG Wenshe Liu 173897 SepRS(2)/pSertRNA(B4)/EF-Sep aminoacyl-tRNA...pEVOL-MmAcKRS1-PylTUUA PylRS M. mazei Bacterial TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(...PylRS M. barkeri azidolysine (AzK) Mammalian TAG Abhishek Chatterjee 217364 pAcBac1-BacMam-4x-PyOtR PyOtR...

...Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx mRuby2...Control Fishell 213829 pAAV_BiCHATe27_dTomato_nlsdTomato BiCHATe27 dTomato/nlsdTomato Control Fishell 213855...Control Fishell 213936 pAAV_BiPVe4_dTomato_nlsdTomato BiPVe4 dTomato/nlsdTomato Control Fishell 213940 ...Control Fishell 213944 pAAV_BiSSTe4_dTomato_nlsdTomato BiSSTe4 dTomato/nlsdTomato Control Fishell Chemogenetics...Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27 Activator ChR2 Fishell 213856 pAAV_BiVIPe4...Activator ChR2 Fishell 213915 pAAV_BiLAMP5e3_ChR2_mCherry BiLAMP5e3 Activator ChR2 Fishell 213937 pAAV_BiPVe4... Activator ChR2 Fishell 213941 pAAV_BiPVe3_ChR2_mCherry BiPVe3 Activator ChR2 Fishell 213945 pAAV_BiSSTe4...