We narrowed to 172 results for: TOX

1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   ...the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto...

2. CRISPR Antimicrobials

   Type

   Blog Post

   ...pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for resistant bacteria. Bikard et al. used...

3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   ...fluorescent dyes of the time was the fact that it was non-toxic and could be expressed in living cells, enabling...

4. Bacterial Expression Systems

   Type

   Collection

   ...Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and visualization inside living...

5. CRISPR Pooled gRNA Libraries

   Type

   Collection

   ...2 plasmid) Knockout Human Moffat 3rd 4 70,948 Toxoplasma Knockout 80636 Knockout T. gondii Lourido N/A...

6. Plasmids 101: Shuttle Vectors

   Type

   Blog Post

   ...to spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing ...

7. Plasmids 101: Stringent Regulation of Replication

   Type

   Blog Post

   ... protein, which can be useful if the product is toxic in high amounts. These plasmids are also useful ...

8. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   ...visualization technique. Ultimately, MG proved to be too toxic for many in vivo applications, but this work proved...

9. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   ...containing the ccdB gene. Since the ccdB product is toxic to the cell, Gateway cloning efficiency can reach...

10. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    ...the Survival of MeCP2-Null Mice without Apparent Toxicity after Intracisternal Delivery. Molecular Therapy...

11. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    ...did so without a substantial immune response or toxicity. More AAV-based CRISPR systems Smaller Cas proteins...

12. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    ...career topics. However, many of the posts were about toxic advisors, fruitless job searches, and even despair...

13. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    ...even kill your cells if your gene of interest is toxic. To combat this, scientists have created synthetic...

14. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type

    Protocol

    ...macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other ...

15. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    ...specificity, so in some cases they can lead to toxicity and off target effects. More recently, shRNA plasmids...

16. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    ...Cas9 is no longer necessary. However, Cas9 can be toxic if continuously expressed (Fu et al., 2013). Using...

17. Hot Plasmids: Summer 2024

    Type

    Blog Post

    ...methyltransferase for epigenetic silencing, which can be toxic, CHARM uses a cofactor fused to a histone tail to...

18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    ...-mouse CD8b-PerCP-Cy5.5), which is present on cytotoxic T killer cells but not dendritic cells. Using ...

19. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    ... can be used, however, these are generally more toxic to cells than their plasmid counterparts. Another...

20. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    ...display assays, ubiquitination activity assays, toxin activity, small molecule binding, growth rate, and...