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We narrowed to 169 results for: TOX

Showing: 121 - 140 of 169 results

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CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 mRNA and...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Type

Blog Post

...the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto...
CRISPR Antimicrobials

Type

Blog Post

...pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for resistant bacteria. Bikard et al. used...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

...fluorescent dyes of the time was the fact that it was non-toxic and could be expressed in living cells, enabling...
Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

... protein, which can be useful if the product is toxic in high amounts. These plasmids are also useful ...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

...visualization technique. Ultimately, MG proved to be too toxic for many in vivo applications, but this work proved...
Plasmids 101: Gateway Cloning

Type

Blog Post

...containing the ccdB gene. Since the ccdB product is toxic to the cell, Gateway cloning efficiency can reach...
5 Reasons to Use Reddit for Science Communication

Type

Blog Post

...career topics. However, many of the posts were about toxic advisors, fruitless job searches, and even despair...
Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

...the Survival of MeCP2-Null Mice without Apparent Toxicity after Intracisternal Delivery. Molecular Therapy...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...did so without a substantial immune response or toxicity. More AAV-based CRISPR systems Smaller Cas proteins...
Plasmids 101: Shuttle Vectors

Type

Blog Post

...to spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing ...
Plasmids 101: The Promoter Region – Let's Go!

Type

Blog Post

...even kill your cells if your gene of interest is toxic. To combat this, scientists have created synthetic...
Fluorescent Protein Guide: Subcellular Localization

Type

Collection

...B4GALT1(1-61) mTurquoise2 Dorus Gadella 68073 GalToxBFP Golgi apparatus GalT signal anchor transmembrane...
AAV Purification by Iodixanol Gradient Ultracentrifugation

Type

Protocol

...macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other ...
CRISPR 101: Homology Directed Repair

Type

Blog Post

... can be used, however, these are generally more toxic to cells than their plasmid counterparts. Another...
Hot Plasmids: Summer 2024

Type

Blog Post

...methyltransferase for epigenetic silencing, which can be toxic, CHARM uses a cofactor fused to a histone tail to...
Antibodies 101: Introduction to Gating in Flow Cytometry

Type

Blog Post

...-mouse CD8b-PerCP-Cy5.5), which is present on cytotoxic T killer cells but not dendritic cells. Using ...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

...display assays, ubiquitination activity assays, toxin activity, small molecule binding, growth rate, and...

Showing: 121 - 140 of 169 results

Results per page:

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Light Sheet Fluorescence Microscopy

AAVs CREATed for Gene Delivery to the CNS and PNS

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

CRISPR Antimicrobials

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Plasmids 101: Stringent Regulation of Replication

Fluorescent Proteins 101: Aptamer Fluorophores

Plasmids 101: Gateway Cloning

5 Reasons to Use Reddit for Science Communication

Rett Syndrome: A History of Research and Therapeutic Outlooks

Overcoming the AAV Size Limitation for CRISPR Delivery

Plasmids 101: Shuttle Vectors

Plasmids 101: The Promoter Region – Let's Go!

Fluorescent Protein Guide: Subcellular Localization

AAV Purification by Iodixanol Gradient Ultracentrifugation

CRISPR 101: Homology Directed Repair

Hot Plasmids: Summer 2024

Antibodies 101: Introduction to Gating in Flow Cytometry

Deep Mutational Scanning with One Pot Saturation Mutagenesis

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