...long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen or a dry ice/ethanol...

...cloning where the genetic changes occur in a test tube. The donor DNA substrate only requires ~50 nucleotides...

...flow-through hybridization assay using a capillary tube, probe DNA-immobilized magnetic beads and chemiluminescence...

...Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation. Plant Cell 24:1478–1493 . https:/...

...surgery (stereotaxic surgery videos can be found on youtube [above] and jove). Delivery of an AAV construct...

...together and then labeled with the antibody in the same tube.     Figure 2: Schematic showing the use...

...Plate readouts can be affected by plate type or tubes, sealing method, and other small variations in consumables...

...ubiquitously expressed protein such as GAPDH or tubulin (sometimes referred to as housekeeping genes). ...can also be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting...

...streamlines the assembly of TALE repeats into a single-tube, 3 day process by utilizing pre-assembled trimers...

...supplied as a mixed population of plasmids in a single tube. Pooled libraries can be small, if they are designed...

...The replication process can also be done in a test tube to copy DNA regions of interest. In vitro DNA replication...

... the water bath as the water evaporates from the tub. Disinfectant may be added to prevent growth of bacteria...bath with individual slots for tubes, remember that when you add the tube to the slots, it can cause the...secure tubes in place and water bath weights can hold bottles in place. After putting your tubes or bottles... water baths that exist as scientists work with tubes and bottles of different sizes in the lab. Some ...water baths contain individual slots for heating up tubes while other water baths hold many liters of water...markers, such as permanent markers, to label your tubes and/or containers that will go into the water bath...chemicals such as ethanol. Writing on the tops of the tubes and lids of items to be placed in the water bath...

...Reagents Opti-Prep (60% Iodixanol) QuickSeal tubes QuickSeal tube spacers 16 ga needle 18 ga needle 10 mL ... formulation buffer) to top off the tube. Seal the QuickSeal tubes. Centrifuge at 350,000 x g for 90 min... QuickSeal tube with a 16 ga needle and start collecting 0.5 mL to 1 mL fractions per tube. Avoid the ...mL fractions in microcentrifuge tubes. Repeat for each QuickSeal tube. The first 3 mL collected corresponds...phenol red Underlay each solution into a QuickSeal tube in the order below using a 10 mL syringe and a blunt...,000 x g at 18 °C. Carefully take the QuickSeal tubes out of the rotor and place them in a stable rack...a row of roughly 20 open 1.5 mL microcentrifuge tubes in a rack. These will be used to collect fractions...

...cannot fit into a single centrifuge tube, aliquot it into several tubes/bottles. Remove the supernatant and...stock culture to a 1.75 mL microfuge tube. Centrifuge in microfuge tube at 10,000 g for 30 sec. Pour off ...proteins and genomic DNA. Incubate tube on ice for 5 min. Centrifuge the tube for 5 min at 12,000 g. Notes:...not available. Vortex microfuge tube for 30-60 sec. Centrifuge the tube for 5 min at room temperature on... (pH 4.8). Invert the microfuge tube to mix. Optional: Place the tube either at -20 °C overnight OR -80...attached to the tube after the 70% ethanol wash. You can also pipet the supernatant out of the tube if you are...resuspended. Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents. The contents...

... mL conical tube on ice pooling the scrapings of two plates into each tube. Keep each tube on ice while...vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes should contain...weigh the empty tube beforehand! If you’ve already gone too far, weigh an identical empty tube. It will be...Reagents 200 µL electrocompetent cells (Default: 4 tubes of Endura Duos, Lucigen, 60242-1) Alternatives include...LB Agar per bioassay plate. 14 mL Vented Falcon Tubes (BD Biosciences, 352059) 3X LB Agar + Antibiotic...growth is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation Prepare,...plates. Prechill Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent cells on ice for 15-20 minutes...

...PCR tube strip into a chilled 96-well freezer block. Add 20 µL of the master mix to each PCR tube. Be ...dispense to the bottom of the tube without collecting drops along the side of the tube. Cap gently - do not push...Bring the PCR tubes to the BSC used for dilutions. Without disturbing the liquid in the tube, gently uncap...uncap the PCR tubes. Add 5 µL of dilutions 6–8 to the appropriate PCR tubes. Pipette back and forth 5 times...times. Lightly cap the PCR tubes. Generate the Droplets Bring the PCR tubes to the droplet generation ...Without disturbing the liquid in the tube, gently uncap the tubes. Add 5 µL of the previously aliquoted... cartridge holder, Bio-Rad, 1863051 8-strip PCR tubes, Axygen, PCR-02-FCP-C ddPCR 96-well PCR plates, ...

...DNA 500 mL vented flask 50 mL conical tubes Microcentrifuge tubes Cell counting chamber slide 30 mL luer-lock...microcentrifuge tube. Vortex the cell suspension. Transfer 10 µL of cell suspension into the microfuge tube containing...Transfer 6 mL of BCD TFX into each of two 50 mL tubes. Cap the tubes and incubate for 1 h in the 37 °C bead bath...transfer the tubes to the biosafety cabinet. Add 180 µg of recombinant antibody plasmid DNA to one tube of 6 ...TFX. Cap the tube and vortex for 5 s to mix. Add 450 µL of 1 mg/mL PEI-MAX to the second tube of 6 mL BCD...Benchtop centrifuge compatible with 50 mL conical tubes Automated cell counter 37 °C, 5% CO 2 incubator ...HEK293 cell suspension into a clean microcentrifuge tube. Pro-Tip Cells settle quickly and need to be resuspended...

... TE solution RNase free tubes: microcentrifuge tubes, 4 mL polypropylene tubes RNase decontamination solution... sample(s).Transfer tissue/cell lysate to a 4 mL tube. Add the following sequentially to 1 mL of lysate...transfer the top, aqueous phase to a new RNAse-free tube. The mixture separates into a bottom organic layer... may have to collect twice or more from the same tube, but unlike using a p1000 tip it will give you more...more control of where you’re aiming your tip in the tube. Precipitate your sample(s). You can use either ...gel-like white pellet of total RNA in the bottom of the tube. Wash the RNA by resuspending the pellet in 0.5–...add RNase-free water or TE as soon as the entire tube is dried but while the white pellet is still visible...

...microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/µL stock into 450 uL PBS in a microfuge tube and vortex...serum albumin (BSA), VWR 10273-264 50 mL conical tubes, VWR 89039-656 96-well polyester (clear) microplate...microplate seal, Thermo Scientific 5701 Microcentrifuge tubes, Neptune 3745.X Pipette tips, 1000 µL, VWR 76322...Dilute purified antigen to 20 ng/µL in a microfuge tube and vortex. Pro-Tip You may need to try a few different... of 1 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. 0.25 ng/µL : Add 450 µL of 0.5 ng/µL...µL stock into 450 µL PBS in a microfuge tube and vortex. 0.125 ng/µL : Add 450 µL of 0.25 ng/µL stock ...stock into 450 µL PBS in a microfuge tube and vortex. 0 ng/µL : 450 µL PBS only. Pipette 50 µL into the first...

...mL microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending on... for simply collecting liquid to the bottom of a tube. You will likely encounter several different types...: September 2022 Equipment Centrifuge Microfuge tubes or other suitable container Personal protective ...microcentrifuge with the lid open, the rotor with microfuge tubes in place, and the control panel where you can set...example of a balanced tabletop centrifuge, with tubes evenly distributed around the rotor. How to Centrifuge...needed. This decision should be based on the size of tubes you are using and the speed at which you need to...example, spinning down samples in 1.5 mL microfuge tubes at 10,000 rpm would require a tabletop microcentrifuge...