We narrowed to 344 results for: ada
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TypeBlog Post...antibody-dependent cellular phagocytosis (ADCP). Figure adapted from van Erp, et al., 2019. Antibody-dependent...Song, WC. Complement and its role in innate and adaptive immune responses. Cell Res 20, 34–50 (2010). https...
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Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog Post..., the ability to embrace serendipity fosters adaptability, a crucial skill for a successful research career...research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...tool for enrichment of ABE-targeted cells By Ellie Adams Single base modifications through ABE or CBE's have... from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian cell lines. ... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor multiple ...- Overview of the neural and gut fiber system (adapted from Sahasrabudhe et al., 2023). To develop... -
What the HEK?
TypeBlog Post...adherent monolayer, though derivatives have been adapted for growth in suspension. Of the many derivatives... allows for large scalability, and can also be adapted for serum-free growth, which significantly reduces... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post... effect is not specific to sucrose, but can be adapted to other sugars such as sorbitol. As sorbitol is...starters, we believe that this approach can be adapted by vector cores and laboratories alike as an easy... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...recombinase-based state machines and how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-... understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert... -
Another Pathway into Cells: iTOP
TypeBlog Post...nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. In certain instances,...mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...Arabia -- as well as closer to home in the US and Canada. In fact, we received so many requests (175 and...imagine, it’s also been quite the administrative headache receiving constant email requests and tracking... -
25 Work From Home Tips for Scientists
TypeBlog Post...career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule... your work schedule as possible so that you can adapt to your kids' needs. Plan ahead. Think about and... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...Nature Communications 2019. Pubmed PMID: 31366908 Adapting sigma54-dependent transcription for CRISPR activation...must be σ54-dependent and the system may not be adaptable to distantly related species from E. coli. This... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...RNA-seq was performed to map TFs to cell fates. (Adapted from Joung et al.) This atlas linked TF ... color coded nominated reference cell types. (Adapted from Joung et al.) Putting MORF to work ... -
Hot Plasmids - January 2023
TypeBlog Post...nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022. Find CRISPR...temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods ... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post... that exist online, but this approach has been adapted for the identification of physical materials that...this Plasmid” section. Having IDs linked to these metadata and publications help to enable reproducibility... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...high school classrooms, but it could be widely adapted and used in a variety of settings. If you are a..., if you are a fellow researcher interested in adapting your research ideas into hands-on educational ... -
A Deep Dive into BioBuilder
TypeBlog Post...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current... BioBuilder book chapter and an illustrated, downloadable classroom poster — with both of these resources... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...intra-molecular annealing. Figure and legend from Okada, 2013. scAAV If your gene of interest is truly...PMID: 20649475. PubMed Central PMCID: PMC2957237. Okada, T. (2013). Efficient AAV Vector Production System... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...customized for protein visualization, relocation, degradation, modification, and scaffolding. In their study...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...for assembling the barcoded gRNA library pool. (Adapted from Wong et al., 2016). With a list of gRNA...assembling barcoded combinatorial gRNA libraries. (Adapted from Wong et al., 2016). With CombiGEM and ... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...in optogenetics, opening up the possibility of adapting GFP lines for optogenetics experiments in specific...transcription activation domains. Both systems are easily adaptable to neuroscience applications, including optogenetics...