...adherent monolayer, though derivatives have been adapted for growth in suspension. Of the many derivatives... allows for large scalability, and can also be adapted for serum-free growth, which significantly reduces...

...tool for enrichment of ABE-targeted cells By Ellie Adams Single base modifications through ABE or CBE's have... from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian cell lines. ...

... effect is not specific to sucrose, but can be adapted to other sugars such as sorbitol. As sorbitol is...starters, we believe that this approach can be adapted by vector cores and laboratories alike as an easy...

...Arabia -- as well as closer to home in the US and Canada. In fact, we received so many requests (175 and...imagine, it’s also been quite the administrative headache receiving constant email requests and tracking...

...career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule... your work schedule as possible so that you can adapt to your kids' needs. Plan ahead. Think about and...

...Nature Communications 2019. Pubmed PMID: 31366908 Adapting sigma54-dependent transcription for CRISPR activation...must be σ54-dependent and the system may not be adaptable to distantly related species from E. coli. This...

...our recent blog post on antibiotic resistance. Degradative enzymes There are less aggressive ways to survive...can’t. To conserve energy, these specialized degradative capabilities are often tightly regulated to be...

...RNA-seq was performed to map TFs to cell fates. (Adapted from Joung et al.)    This atlas linked TF ... color coded nominated reference cell types.  (Adapted from Joung et al.)    Putting MORF to work ...

...nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022.   Find CRISPR...temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods ...

... that exist online, but this approach has been adapted for the identification of physical materials that...this Plasmid” section. Having IDs linked to these metadata and publications help to enable reproducibility...

...high school classrooms, but it could be widely adapted and used in a variety of settings. If you are a..., if you are a fellow researcher interested in adapting your research ideas into hands-on educational ...

...Chem Neurosci 6:476–484. PMID: 25587888 Coward P, Wada HG, Falk MS, Chan SD, Meng F, et al (1998). Controlling...451–460. PMID: 15765260 Farrell MS, Pei Y, Wan Y, Yadav PN, Daigle TL, Urban DJ, Lee H-M, Sciaky N, Simmons...Shimojo M, Zhang M-R, Tomita Y, Nakahara J, Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T (2020...

...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current... BioBuilder book chapter and an illustrated, downloadable classroom poster — with both of these resources...

...in optogenetics, opening up the possibility of adapting GFP lines for optogenetics experiments in specific...transcription activation domains. Both systems are easily adaptable to neuroscience applications, including optogenetics...

...few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting the appropriate...single gRNA-expressing plasmid, but it can also be adapted to express multiple gRNAs. Addgene has two Golden...

...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...for using CRISPRi in many types of bacteria: lab-adapted, environmental isolated, and disease-associated...

...customized for protein visualization, relocation, degradation, modification, and scaffolding. In their study...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence...

...intra-molecular annealing. Figure and legend from Okada, 2013.   scAAV If your gene of interest is truly...PMID: 20649475. PubMed Central PMCID: PMC2957237. Okada, T. (2013). Efficient AAV Vector Production System...

...include a lot more. Font size: Making your poster readable While we are talking about font, remember this...this: bigger is better! Your poster should be readable from several feet away. It can also be useful to ...

...longevity in eukaryotes and promote transcript degradation in prokaryotes.  Prokaryotic termination   Prokaryotic...important for stability of the mRNA, protection from degradation, and is integral to the nuclear export and translation...