Skip to main content

We narrowed to 351 results for: cki

Showing: 121 - 140 of 351 results
  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...and so was really starting from the beginning. Luckily, a fellow graduate student and a data analyst in...comment in C++ and Java Command line history: Tracking command line history by recording all commands...
  2. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...the features that would define a good tool for sticking together biological components. Nearly all of ...full advantage and have lots of fun building and clicking. References and Resources References Devaraj...
  3. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...HA staining is weaker than reporter staining. Picking the right reporter for your RANBody While all three... with these plasmids from Penn State Need help picking the right fluorescence microscopy technique to ...
  4. Antibodies 101: Conjugation

    Type
    Blog Post
    ...Hegazy, et al., 2020; Niemeyer, et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for ...–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous...
  5. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...manipulate full genes and observe the effects. Luckily for us, CRISPR is an incredibly versatile system...et al. 2014).  Simple knock-out experiments are trickier when it comes to long non-coding RNAs (lncRNA)...
  6. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction digest...design. Note that you can browse all components by clicking the blue text in the upper right-hand corner.  ...
  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...Epigenetic engineering with single-pot assembly Tracking cell-cell interactions New auxin inducible degron...human body and G-baToN provides versibility in tracking and modulating the interactions between cells....
  8. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...tweet in order to easily access it later. Pro tip Checking the number of retweets, likes, and comments on...altmetric score, is also displayed in your feed. By clicking on a paper in your feed you can see suggested ...
  9. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...
  10. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ... localization, cell structure, intracellular trafficking, and protein turnover rates. Additionally, by...fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery of many ...
  11. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...cloning rings. A picture of a 60 mm dish ready for picking is shown in figure 1.   Find Additional Useful ...firmer 2% agarose gel solution discussed above for picking. Many thanks to our guest blogger Iris Lindberg...
  12. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...While many barcoding libraries are available for tracking cell lineage, different applications require careful...recently developed an approach called Simultaneous Tracking of Recombinase Activation and Clonal Kinetics ...
  13. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...described in the preceding sections are simple ways of knocking out a non-essential gene of interest. However,...Although CRISPR is very good at making knockouts, knocking in large sections of DNA can be more difficult...
  14. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... side, successful indel generation and epitope knockin was achieved by a few respondents suggesting that...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...
  15. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...geared toward careers in academia or industry. Luckily, I discovered that there are many ways to get a...of others were key to creating my career path. Luckily, in grad school much of the up front work was done...
  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... this limitation, with limited success so far. Luckily for patients, this gene can be targeted to RPE ...Forever Fix: Gene Therapy and the Boy Who Saved It by Ricki Lewis. ISBN-13: 978-1250015778 7. Yu W and Wu Z....
Showing: 121 - 140 of 351 results