We narrowed to 209 results for: dap

1. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   ... that exist online, but this approach has been adapted for the identification of physical materials that...

2. Visualizing Genomic Loci with CRISPR-Sirius

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   Blog Post

   ...without changing them. Thus, it has been widely adapted for a wide variety of applications: base editing...

3. Visualizing Translation at the Single Molecule Level

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   Blog Post

   ...disrupting ribosome-mRNA complexes. Recent work has adapted this technique to monitor local translation and...

4. "Build Your Plasmid": The Game - Play to Teach

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   Blog Post

   ...for non-commercial reuse. There should be ways to adapt the game to each a variety of subjects at many different...

5. 5 Great Apps for Lab Life

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   Blog Post

   ...student, I have relied more and more heavily on adapting my smartphone to help me with my research in terms...

6. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   ...and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein / Rhodamine / Cy5 (e.g. Semrock's)...

7. Western Blot

   Type

   Protocol

   ...protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches. Last ...

8. CasPEDIA: A Functional Classification of Cas Enzymes

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   Blog Post

   ...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al.   You can also search by ...

9. Hot Plasmids - February 2022

   Type

   Blog Post

   ...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...

10. Antibodies 101: Isotypes

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    Blog Post

    ... constant domains. This switching helps B cells adapt during the course of an immune response since it...

11. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post

    ... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes...

12. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...

13. A Deep Dive into BioBuilder

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    Blog Post

    ...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current...

14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    ...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...

15. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    Blog Post

    ...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4...

16. Luminescent Imaging with Nano-lanterns

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    Blog Post

    ...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-...

17. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...

18. Plasmids 101: The protein expression toolbox

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    Blog Post

    ... Fig. 3 dTag mechanism of protein degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral...

19. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence...

20. Summer SciComm: Preprints

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    Blog Post

    ...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...