Addgene

We narrowed to 196 results for: dap

Showing: 121 - 140 of 196 results

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Luminescent Imaging with Nano-lanterns

Type

Blog Post

...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-...
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Type

Blog Post

... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al. You can also search by ...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...
Fluorescence Titering Assay

Type

Protocol

...protocol was developed for 293T cells but can be adapted to a variety of target cell lines and selection...
Plasmids 101: The protein expression toolbox

Type

Blog Post

... Fig. 3 dTag mechanism of protein degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral...
Summer SciComm: Preprints

Type

Blog Post

...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence...
Plasmids 101: Cre-lox

Type

Blog Post

...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...
General Transfection

Type

Protocol

...HEK293T cell line should work. This approach can be adapted for different cell lines and different transfection...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Type

Blog Post

...protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to work in your lab. Find...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021. Let me leave...
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Type

Blog Post

...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...
Hot Plasmids February 2024

Type

Blog Post

...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission. Find...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Type

Blog Post

...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

Showing: 121 - 140 of 196 results

Results per page:

Luminescent Imaging with Nano-lanterns

Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

CasPEDIA: A Functional Classification of Cas Enzymes

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Fluorescence Titering Assay

Plasmids 101: The protein expression toolbox

Summer SciComm: Preprints

Truncated gRNAs for Regulating Gene Expression

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Plasmids 101: Cre-lox

General Transfection

Adenoviral Vector Production and Troubleshooting

Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Hot Plasmids February 2024

Cpf1 Update: Comparison to Cas9 and NgAgo

Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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