...alindromic R epeat (CRISPR) systems, which form an adaptive immune system in bacteria, have been modified ...target. Due to its comparative simplicity and adaptability, CRISPR has rapidly become the most popular ...recruit activation proteins. CRISPRi has also been adapted for diverse bacterial species using a modular system...

... Most IF techniques use a nuclear stain, such as DAPI, to identify individual cells. These stains also...BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647 Antibody Selection...

...alindromic R epeat (CRISPR) systems, which form an adaptive immune system in bacteria, have been modified ...

...transcribes genes encoding transfer RNAs (tRNA), the adaptor molecules that are responsible for bringing amino...

... website. Ligation Independent Cloning (Figure adapted from LIC protocol ) Back to Top Yeast-mediated ...