Immunocytochemistry
Type
Protocol
...Triton X-100 in 50 mL PBS. 300 nM DAPI working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1... of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution...500 µL of 300 nM DAPI working solution for 10 min at RT in the dark. Remove the DAPI and dispose of it...Paraformaldehyde 5 mg/mL 4′,6-diamidino-2-phenylindole (DAPI) Bovine serum albumin (BSA) Triton X-100 Primary...solution by diluting 5 µL of the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section...