...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) to...fluorescence intensity of the cpGFP without altering that of the internal control (Fig 1). Because this method...for doing gene targeting, reporter generation, controllable gene knockouts, and inducible gene inactivation...design. B) Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview. Image...

... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging...FLAMP1 and FLAMP2 Chu Lab FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other...

...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...provide important details including the protein and plasmid sequence, the antigen sequence, and cross-species...develop and refine these application lists. The plasmids we use to produce antibodies are available as ...as part of Addgene’s larger Antibody Plasmid Collection , which also includes other types of affinity reagents...popular markers like tubulin or epitope tags like Myc, GFP, and more. Neuroscience : Antibodies targeting proteins...? Check out our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or email...

... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits...system for controlled gene expression in Caenorhabditis elegans. - Jane Hubbard Lab. Plasmids for the temporal...

...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1...Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent...proteins, including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors... 140555 pAAV-hsyn-GRAB_DA-mut Syn GRAB_DA-mut (control) none Constitutive 9 Li 140556 pAAV-hsyn-GRAB_rDA1m...140558 pAAV-hsyn-GRAB_rDA-mut Syn GRAB_rDA-mut (control) none Constitutive 9 Li 208698 pAAV-hSyn-GRAB-gDA3m...pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE GFAP iGABASnFR2 (control) none Constitutive 1, 5 GENIE 218874 pGP-AAV-syn-iGABASnFR2...pGP-AAV-syn-iGABASnFR2(no bind)-WPRE Syn iGABASnFR2 (control) none Constitutive 1, 5 GENIE 218877 pGP-AAV-syn-flex-iGABASnFR2...

...(4; brighter than control, 3; same as control (Cetn1/sgRNA1), 2; darker than control, 1; very dark). Brighter...pCAG-EGXXFP plasmid along with the individual gRNAs in their PX330 plasmids (or similar plasmids for gRNA...reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA plasmid (Mashiko et... (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences ...2013). The pCAG-EGxxFP target plasmid contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous... multi-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized Cas9 expression...inserted, disrupting the EGFP fluorescent signal, for details on this plasmid, see Mashiko et al., 2013...

...lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF 2.3 MB Zhang GeCKO pooled...lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Kits are also available (...vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (...collaboration with the labs who have deposited CRISPR plasmids , we've created a series of links and guides to...on Addgene's blog Protocols Lab(s) Description Plasmids in protocol Download protocol Church gRNA design...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF 104.1 KB Chen and Wente Zebrafish: gRNA cloning...(Cre recombinase) PDF 102.5 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators...

...parental cell line, but not in the iso-genic knockout control. This can involve cell surface labeling and/or ... target of interest, you can add an additional control containing treated cells. The expected change in... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...

...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... Plasmid Collections Fluorescent Proteins FRET Fluorescent...these types of studies Addgene has a collection of plasmids suitable for creating individual fluorescently...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion protein...of interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1...

...recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page for more information .../Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP 9, rg* Wilson 105558 pENN.AAV.CamKII...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....2, 5, 8, 9, rh10 Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre...EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB Wilson...

...recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls the activity...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...Hahn's lab at the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 ...studying cellular dynamics. The photoinducible control of protein function is more specific and much faster... the same number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches ...the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based quantification...

...was developed by Shannon Boye’s lab. Plasmid Information The plasmid below is available in each of the above...pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...production , these viral vectors undergo quality control, including AAV titration by ddPCR, in vitro and... preparations were produced with the pTR-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated...above serotypes and buffers. Please click on the plasmid name for ordering information. ID Name Promoter...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...smaller) plasmids. Finally, having four separate plasmids allows researchers to partially control expression...encoded on separate plasmids and are co-transfected into cells alongside a plasmid encoding the minigenome...four viral genes in the same plasmid (as opposed to four separate plasmids), there are a few reasons arguing...more technical standpoint, a plasmid with all four genes and necessary control elements for each would be... 2 μg minigenome plasmid per well (in a 6 well plate format). However, since plasmid quality and transfection...system uses plasmids where the genes (either viral genes or minigenome) are under control of a T7 RNA ...relatively large. Cloning the viral genes in this plasmid would be more difficult compared to if each gene...

... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd... empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table...packaging plasmids that can be used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation...

...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant... table highlights plasmid collections that can be used to create and express plasmid-based antibodies....Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...sort buttons to find antibody plasmids for: Common antigens such as GFP or mCherry Monoclonals, Nanobodies...) ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that ...collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid... Plasmid Collections...

...receive transfer plasmid DNA that you can use along with lentiviral packaging plasmids to create your own...targeting EGFP, BRAF, and MAP3K4. The gRNAs targeting EGFP can be used as positive controls for gene editing...rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene...helped distribute many of these technologies in plasmid form and are proud to have facilitated their fast...fast adoption. However, in many cases the plasmids themselves are only the starting point for the production... use; after receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, ...from your primary goal. Getting these quality-controlled reagents from Addgene can greatly expedite a ...

... check out our Biosensors Plasmid Collection . To find optogenetics plasmids available through Addgene...mutagenesis - use Addgene's Optical Switch Plasmid Table to search for plasmids for your next experiment. For a ...domain (iLID plasmid), while its natural binding partner SspB is included in a separate plasmid; Blue light...dimerization tags (TULIPs), plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb ...Blue-light photoreceptor for light-controlled gene expression. These plasmids rely on the engineered two-component...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin...research utilize light to detect, measure, and control molecular signals and cells in order to understand...

...Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells. You...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ...Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All...

...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...than 10 plasmids but we recommend using the spreadsheet option for deposits of 10 or more plasmids.   ...

...Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools...cytosine demethylation in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation...Tet1CD, is available from Rudolf Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure...Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1...experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   Leah Schwiesow...510–517 . https://doi.org/10.1038/nbt.3199 Find plasmids from this publication at Addgene. Klann TS, Black...