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  1. Hot Plasmids - January 2023

    Type
    Blog Post
    ... Monoclonal antibodies can yield relatively low signal in immunoassays due to only having one binding ...you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal, with ...

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ... set, we would expect TagRFP to give a brighter signal than mCherry. In general, filter sets designed ...all organisms and that if you want the brightest signal, you may need to try several proteins in your system...

  3. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...technique can be applied to an important neuronal signalling pathway - if you’ve got a useful tool, there’...neuronal networks, and selectively activating signaling pathways. Many scientists came to the Addgene ...

  6. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...antibody is ideal as it will help to amplify the signal. Some applications use either direct (primary-conjugated..., remember that a secondary should be used when signal amplification is needed.  Species  Next, check ...

  8. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...Gutless Ad maintain only the ITRs and the packaging signal (Ψ), both of which are essential for assembly of...sequences and retain only the ITRs and packaging signal in cis. Therefore, co-infection with a helper adenovirus...

  11. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because of its...pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators...

  13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...light to detect, measure, and control molecular signals, cells, and groups of cells in order to understand... into distinct populations based on fluorescent signal. Thus, FACS can be used to separate cells expressing...

  15. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat an ELISA plate...during incubation steps in order to enhance the OD signals and then complain about high background. In many...

  17. Five Popular Model Organisms

    Type
    Blog Post
    ...including the nervous system, development, cell signaling, aging, and mechanotransduction to name a few....Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid that...

  18. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...binding sites found on avidin are also ideal for signal amplification during these experiments. During ...interest leading to amplification of the reporter signal and easier detection. This strategy is referred...

  19. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...SunTag scaffold, the intensity of the fluorescent signal is boosted and enables single molecule tracking... T cell receptor portion of the CAR facilitates signal transduction and release of cytotoxic granules ...
Showing: 121 - 140 of 260 results