We narrowed to 269 results for: gnal

...technique can be applied to an important neuronal signalling pathway - if you’ve got a useful tool, there’...neuronal networks, and selectively activating signaling pathways. Many scientists came to the Addgene ...

...stable fluorescence signal, showing no or little deterioration or change of the signal during the course...

...antibody is ideal as it will help to amplify the signal. Some applications use either direct (primary-conjugated..., remember that a secondary should be used when signal amplification is needed.  Species  Next, check ...

...fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule in the brain. “We hope that iGluSnFR3...these neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine administration...

...Gutless Ad maintain only the ITRs and the packaging signal (Ψ), both of which are essential for assembly of...sequences and retain only the ITRs and packaging signal in cis. Therefore, co-infection with a helper adenovirus...

...localizes primarily to the cell body with very little signal detected along the neurite (Figure 1.) Moreover...than other dark fluorescent proteins, it has good signal stability allowing for minimized basal FRET variability...

...conditions that give the strongest biotinylation signal, as measured via Western blot, and that result ... fusion protein and saturation of biotinylation signal. For Split-BioID, Schopp et al allowed 24 hours...

... an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because of its...pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators...

...protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/threonine-... an integration point for multiple biochemical signals. In collaboration with numerous research labs from...

...inhibit engineered receptors that affect cell signalling pathways. Luciferase Plasmids Use bioluminescent...bioluminescent reporters for studying gene expression, signaling pathways, and more. Learn More and Explore Addgene...

...or above 650 nm (far-red or near-infrared), as signals below 650 nm are highly absorbed by hemoglobin ...hemoglobin and cause undesirable background signals. Many near-infrared fluorescent proteins are derived from bacterial...

...decreasing the fluorescent signal from the first FP and increasing the signal from the second FP. Several...

...light to detect, measure, and control molecular signals, cells, and groups of cells in order to understand... into distinct populations based on fluorescent signal. Thus, FACS can be used to separate cells expressing...

... was reading. SHH is involved in the hedgehog signalling pathway, which is a key regulator of all bilateral...development. Specifically, SHH is a morphogen, a chemical signal that diffuses down a concentration gradient exerting...

...-expressing E. coli. The GV-specific ultrasound signal inside the tumors was overlaid with the anatomical... in situ expression. The GV-specific ultrasound signal is in hot and the anatomical B-mode images are ...

...Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat an ELISA plate...during incubation steps in order to enhance the OD signals and then complain about high background. In many...

...with high specificity for critical axon guidance signaling proteins and growth cone receptors. Developed ...conserved proteoglycans crucial for regulating cell signaling during development. The IPI collection includes...

...binding sites found on avidin are also ideal for signal amplification during these experiments. During ...interest leading to amplification of the reporter signal and easier detection. This strategy is referred...

...minutes without any issues in toxicity. TurboID’s signal is significantly greater than miniTurbo at earlier...exhibits 1,000 fold faster kinetics and increases the signal to noise ratio 30-fold compared to the standard...

...including the nervous system, development, cell signaling, aging, and mechanotransduction to name a few....Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid that...