... cargo (DNA or RNA) to target cells has been challenging. A variety of vector delivery technologies have...treatment appears to be both safe and effective. Challenges for AAV gene therapy Although AAV-based gene ...promising, scientists are working to overcome key challenges to their efficacy:  Pre-existing immunity: Many...

...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...available planarians: Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis....differ. For example, Girardia sp. and Dugesia dorotocephalacan can be fed dsRNA-expressing E. coli that are...

...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc...small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed a polymerase...

...which creates a challenge when studying them since their complete knockdown is lethal. Incomplete or titratable... to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria...

...infected host cell is eventually lysed. Cell lysis halts protein production, but there are non-lytic insect... however, are expensive and very technically challenging to use. Alyssa D. Cecchetelli is a Scientist... expression in Escherichia coli: advances and challenges Production of recombinant proteins in plant root...

...be split into two halves, which are non-fluorescent on their own. If the two halves are in close proximity...determine the proximity of two proteins fused to the halves of the split FP. This technique is also is also...

... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...Adeno-Associated Viral Vector-Mediated Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https:/...

...hematopoietic disorders sickle cell anemia and beta-thalassemia. Lentiviral vectors are promising agents for ...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...HMGA2 activation after gene therapy of human β-thalassaemia. Cavazzana-Calvo M, Payen E, Negre O, Wang G...

...gRNA together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb...transgenes might seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In...WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang...

...RNA is not inherently fluorescent and can be challenging to track intracellularly, but aptamers permit... when bound to DFHBI despite being approximately half the length (Warner et al., 2014); its length reduces...next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split aptamer for visualizing...

...become bored because I was not being mentally challenged. For these and other reasons, I started  looking...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like...

... et al., Science. 2014. Jan 3;343(6166):80-4. Shalem et al., Science 2014. Jan 3;343(6166):84-7. Koike-Yusa...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They...implicated in disease progression. One of the major challenges in signaling research is the inability to replicate... (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, ...

...That’s what makes it so wonderful. The most challenging part of jumping into this career path from academia... your hair out, your successes, failures, and challenges you’ve overcome. This information may help you...completely new field without issue. This may be more challenging if you are trying to apply for an outright job...

... lymph nodes at different times after antigen challenge, scientists were able to track where GFP labelled...et al. 2016, Cavanagh et al. 2008)? Technical challenges of two photon microscopy Intravital imaging is... at steady state or under physio-pathological challenge. However technical specificities have to be taken...

...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras." Cell 168.5 (2017...

...the University of California Berkeley. It’s been half a year since the march for science on April 22, ...present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be a simple, unquestioning... fingers of skepticism to defend an agenda that halts current data collection, destroys existing data,...

... This is the first half of a two-part interview with Vini Mani and Amy Gilson from Science in the News...eager to communicate their science. In this first half of our two-part interview with Vini and Amy, we’...

...kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex. The Covert...which fluorophores can be multiplexed together. Challenging  to Design: FRET reporter activity depends on...

... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk...

...This is the second half of a two-part interview with Vini Mani and Amy Gilson from Science in the News...involved in science communication. In this second half of our conversation with Vini Mani and Amy Gilson...