We narrowed to 318 results for: idua
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TypeCollection...construct and can be accessed using the links to view individual plasmid webpages. These plasmids are described...
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Zhang Lab CRISPR Page
TypeCollection...amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...multiple inserts, enter details for each insert individually. Figure 9: Add cloning information for the...for your first plasmid and can be used on each individual page (Gene and Insert, Cloning Information, Growth... -
Bacterial Expression Systems
TypeCollection...information you need. If you want to dissect an individual protein’s function under a wide range of highly... -
CRISPR Pooled gRNA Libraries
TypeCollection...information about each library can be found on the individual library page. Find Pooled Libraries of Interest... -
Delivery Methods for Generating iPSCs
TypeBlog Post...Insertional mutagenesis, transgene reactivation, residual expression Lentivirus Integrating High Low ...Stable Genome integration Insertional mutagenesis, residual expression piggyBac Excisible Medium Medium... -
Deep Dive: qPCR
TypeBlog Post...target in the reaction should be compared to an individually run sample when developing the protocol and ...and optimized until the multiplexed and individual values match across the board. If the samples or sample... -
15 Hot Plasmids from 2017
TypeBlog Post...fusion protein retained the PAM properties of the individual Cas9. Kim et al. also mutagenized the cytidine...proposed to be essential in B. subtilis 168. Each individual strain includes a xylose-inducible dCas9 paired... -
Technical Design of a Western Blot
TypeBlog Post...the manufacturer, each rAb in a mixture can be individually validated. rAbs are not as commonly available...fast (often <10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They work well... -
Molecular Biology Reference
TypeGuide...Each bacterium can contain multiple copies of an individual plasmid, and ideally would replicate these plasmids... of E. coli used today are descended from two individual isolates, the K-12 strain and the B strain. K... -
Addgene's 20th Anniversary Party
TypeBlog Post...and more. It's this diverse group of talented individuals that makes Addgene — well, Addgene. We're so ... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog Post...the students to separate out colonies from each individual strain using the streak plate technique. Time... -
Congratulations, Deck The Lab winners!
TypeBlog Post...place, the BTC Institute decorated with dozens of individual virus snowflakes, since snowflakes and viruses... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog Post...The immortalized cells, called hybridomas, are individually isolated and expanded. The clonal cells are ... -
Bringing Sustainable Practices to the Lab: Easy Action Items
TypeBlog Post... how can one little change make a difference? Individual changes to our behavior can lead to cultural ... -
500,000 Plasmids Shipped and Counting!
TypeBlog Post... the daily workings in Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post...FinchTV(Mac/PC) Chromas (PC) 2. You should see individual, sharp and evenly spaced peaks …like these…. ... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions... -
Editor's Choice, September 2016
TypeBlog Post...CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats Better Dyeing Through Chemistry... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and MCF10A...