We narrowed to 191 results for: lenti

...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

...more. Convert iPSCs to neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:...transcription factors in a single polycistronic lentiviral vector . (Addis et al. PLoS One. 2011) See More...

... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...expressing Cas9. A549 cells were transduced with lentiCas9-Blast 1 and then selected with 1 μg/mL blasticidin...lines generated from A549 cells transduced with lentiCas9-Blast 1 . A549 cells were transduced (MOI = 37...control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid...

...2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world. In the ...

...Our CRISPR Guide Pages Find Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership...

...Addgene would be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - ...

...cell types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables...

...CRISPR-Cas9: Tips for Optimizing sgRNA Activity Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

... and requests are still pouring in! While our lentiviral service is going strong, the AAV service has ...

...et al., 2018). From this data, they generated lentiviral constructs using the High-Throughput Mutagenesis...

...CRISPR-Cpf1(Cas12a) Double Knockout Library is a lentiviral library targeting 26 genes chosen from an analysis...

...mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown,...

...comprehensive transcription factor library, in lentiviral vectors. At 3500 plasmids, this represents the...

...unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used plasmids made...

...basic mammalian expression system and go for a lentiviral or a retroviral backbone. This is considered ...

...deaminases like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and ...

...specific DNA sequence in only 1 min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9...

...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation...

...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...

... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...