We narrowed to 192 results for: lenti

1. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

   Type

   Protocol

   ...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

2. Isolating a Monoclonal Cell Population by Limiting Dilution

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   Protocol

   ... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...expressing Cas9. A549 cells were transduced with lentiCas9-Blast 1 and then selected with 1 μg/mL blasticidin...lines generated from A549 cells transduced with lentiCas9-Blast 1 . A549 cells were transduced (MOI = 37...control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid...

3. Viral Prep Service for Plasmids and Pooled Libraries

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   Collection

   ... libraries. Viral preparation options include lentivirus and AAV. ...immediately after MTA approval. Browse AAV Browse Lentivirus Packaged on Request Choose from 30,000+ options...

4. Adenovirus Plasmids

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   Collection

   ...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

5. Addgene Protocols Go Viral: AAV Purification and Titration

   Type

   Blog Post

   ...2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world. In the ...

6. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

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   Blog Post

   ...Our CRISPR Guide Pages Find Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership...

7. Fluorescent Protein Guide: Subcellular Localization

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   Collection

   ...Ciliary Base Chibby1 GFP Ken-Ichi Takemaru 89770 pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base ...Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru 122871...

8. Podcast: Interview with Addgene's Founders

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   Blog Post

   ...Addgene would be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - ...

9. Performing In Vivo CRISPR Screens Using the FITS Approach

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   Blog Post

   ...cell types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables...

10. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type

    Blog Post

    ...CRISPR-Cas9: Tips for Optimizing sgRNA Activity Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

11. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type

    Blog Post

    ... and requests are still pouring in! While our lentiviral service is going strong, the AAV service has ...

12. A new platform for fast functional characterization of potentially cancerous mutations

    Type

    Blog Post

    ...et al., 2018). From this data, they generated lentiviral constructs using the High-Throughput Mutagenesis...

13. Supporting Rare Disease Research with BeHeard 2017

    Type

    Blog Post

    ...mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown,...

14. What's New in CRISPR - September 2019

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    Blog Post

    ...CRISPR-Cpf1(Cas12a) Double Knockout Library is a lentiviral library targeting 26 genes chosen from an analysis...

15. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post

    ...comprehensive transcription factor library, in lentiviral vectors. At 3500 plasmids, this represents the...

16. London Calling - Addgene’s New Europe Office in London

    Type

    Blog Post

    ...unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used plasmids made...

17. Choosing Your Perfect Empty Backbone

    Type

    Blog Post

    ...basic mammalian expression system and go for a lentiviral or a retroviral backbone. This is considered ...

18. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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    Blog Post

    ...deaminases like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and ...

19. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    ...specific DNA sequence in only 1 min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9...

20. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type

    Blog Post

    ...after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that...