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We narrowed to 174 results for: lentiviral plasmid

Showing: 121 - 140 of 174 results

  2. CRISPR Library Amplification

    Type
    Protocol
    ... here ). Lentiviral plasmids can recombine between their LTRs resulting in a smaller plasmid containing...differences, and plasmid recombination can all impact the representation of individual plasmids in the pooled...protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells. Protocols... protocol allows the amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries...libraries contain tens to millions of different plasmids in a single sample. They are often used for screening...throughput multiplexed experiments. These pooled plasmids differ by only a short region of DNA: guide RNAs...results for libraries up to 200,000 individual plasmids and can be scaled according to the actual number...

  3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How to Design ...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also ...theoretically possible, but likely more challenging, with lentiviral expression. For more detail on how to design ...Resources on Addgene.org Find fluorescent protein plasmids and resources Visit our Molecular Biology Reference...

  4. CRISPR Plasmids and Resources

    Type
    Collection
    ... of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety .... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...2016). Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular...

  7. Adenovirus Plasmids

    Type
    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

  8. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...percentage of surviving cells may be the result of plasmid integration via recombination during transfection...out Our Primer for Beginners! Learn All about Lentiviral Vectors Additional Resources on the Addgene....

  9. Retrovirus Plasmids

    Type
    Collection
    ...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

  10. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line. This...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...Guide or browse our collections of optogenetics plasmids – and keep an eye out for future updates to the...on Addgene.org Optogenetics Guide Optogenetics Plasmids References Aljiboury, A. A., Ingram, E., Krishnan...

  11. CRISPR Guide

    Type
    Collection
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...

  12. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...this gene can be targeted to RPE cells using a lentiviral vector and a clinical trial is ongoing. Moving...in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral...other viral vectors such as adenovirus (AdV) and lentivirus (LV) are known to transduce retinal cells, AAV...

  13. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same ...Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find...same family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...

  14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ..., et al., 2006). AAV2 capsids are available as plasmids in the repository and can be selected as a serotype..., et al., 2006). AAV8 capsids are available as plasmids in the repository, as part of our in-stock viral...retinal cells (Issa et al., 2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors are not...Kidney by Adenovirus, Adeno-Associated Virus, and Lentiviral Vectors After Intravenous and Direct Kidney Injections...Request Browse all in-stock AAV preps Browse all AAV plasmids ...

  15. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer and ...between CRISPR components expressed from different plasmid backbones. They noted that backbone differences... as the order, or distance in the placement of plasmid elements can impact expression levels and consequently...plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters or fluorophores are ...Collection More resources on the Addgene blog Plasmids 101: Golden Gate Cloning High Complexity Golden...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...

  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... guide (synthetic, in vitro transcription, or lentiviral delivery) can affect the accuracy of a predictive...CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries...

  18. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid electroporation (episomal... [7, 8] and mRNA transfection [9]. Many of the plasmids used for these methods can be found on Addgene...Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...that Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources...Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab ...

  19. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... Pages Find CRISPR Plasmids for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175...

  20. Neurodegeneration Research Collection

    Type
    Collection
    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...
Showing: 121 - 140 of 174 results